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S100B Antibody (MA5-50641) in IHC (P)
Immunohistochemistry of S100B in paraffin-embedded human malignant melanoma tissue. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with S100B recombinant monoclonal antibody (Product # MA5-50641) with a dilution of 1:1,000 for 1 h... View More
Product Details
MA5-50641
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
PD00-11
Immunogen
Full length corresponding to Human S100 beta aa 1-92/92.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_3092952
Product Specific Information
Positive control: Human malignant melanoma tissue, human meningioma tissue.
Predicted band size: 11 kDa
Subcellular Location: Cytoplasm, Nucleus.
Target Information
S100B is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells and are involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100B may function in neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Chromosomal rearrangements and altered expression of this gene have been implicated in several diseases, including Alzheimer's disease, Down's syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Protein S100-B; S-100 calcium-binding protein, beta chain; S-100 protein beta chain; S-100 protein subunit beta; S100 calcium binding protein beta (neural); S100 calcium-binding protein B; S100 calcium-binding protein, beta (neural); S100 protein beta chain
Gene Aliases: NEF; S100; S100-B; S100B; S100beta
UniProt ID: (Human) P04271
Entrez Gene ID: (Human) 6285
axonogenesis
central nervous system development
learning or memory
memory
cell proliferation
positive regulation of cell proliferation
regulation of cell shape
positive regulation of apoptotic process
positive regulation of I-kappaB kinase/NF-kappaB signaling
innate immune response
regulation of neuronal synaptic plasticity
astrocyte differentiation
response to glucocorticoid
response to methylmercury
long-term synaptic potentiation
cellular response to hypoxia
negative regulation of skeletal muscle cell differentiation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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