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SALL1 Antibody (PA5-115864) in ICC/IF
Immunocytochemistry analysis of SALL1 in A549 cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115864). Secondary staining was applied with mouse anti-beta tubulin (1 hr at 37°, AlexaFluor 594 conjugated goat anti-rabbit IgG (Red), AlexaFluor 488 conjugated goat anti-mouse IgG (Green) and DAPI.
Product Details
PA5-115864
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human SALL1(Accession Q9NSC2), corresponding to amino acid residues R4-E54.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2900498
Product Specific Information
Antibody detects endogenous levels of total SALL1.
Target Information
Sall1, which encodes a zinc finger protein, functions as a transcriptional repressor and interacts physically with histone deacetylase and other components of the chromatin remodeling NuRD complex. It is unknown whether the transcriptional repression is solely dependent on histone deacetylase activity. Gene expression profiling has identified Sall1 as a microglial signature gene. Microglia are the resident macrophages of the central nervous system (CNS). Sall1 is also expressed in abundance in the mesenchyme-derived structure from condensed mesenchyme, S-comma-shaped bodies, to renal tubules and podocytes. Sall1 has been identified as a key transcription factor in self-renewal renal progenitor cells. Sall1 is required to maintain the stemness of nephron progenitor cells by restraining their differentiation into renal vesicles. Defects in SALL1 are the cause of Townes-Brocks syndrome as well as bronchio-oto-renal syndrome. Heterozygous mutations of human SALL1 leading to Townes-Brocks syndrome features dysplastic ears, preaxial polydactyly, imperforate anus, and less commonly, kidney and heart anomalies (Kohlhase et al. 1998). Two transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: epididymis secretory protein Li 89; HSal1; sal-3; sal-like 1; Sal-like protein 1; Spalt-like transcription factor 1; Zinc finger protein 794; Zinc finger protein SALL1; Zinc finger protein Spalt-1; zinc finger protein Spalt-3
Gene Aliases: HEL-S-89; HSAL1; Msal-3; Sal-1; SAL1; SALL1; TBS; ZNF794
UniProt ID: (Human) Q9NSC2
Entrez Gene ID: (Human) 6299, (Rat) 307740, (Mouse) 58198
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
histone deacetylase activity
protein binding
beta-catenin binding
sequence-specific DNA binding
transcription regulatory region DNA binding
metal ion binding
RNA polymerase II transcription factor binding
RNA polymerase II transcription coactivator activity
enhancer sequence-specific DNA binding
molecular_function
chromatin binding
nucleic acid binding
C2H2 zinc finger transcription factor
negative regulation of transcription from RNA polymerase II promoter
ureteric bud development
branching involved in ureteric bud morphogenesis
kidney development
ventricular septum development
mesenchymal to epithelial transition involved in metanephros morphogenesis
transcription, DNA-templated
heart development
gonad development
histone deacetylation
olfactory nerve development
olfactory bulb interneuron differentiation
pituitary gland development
neurogenesis
positive regulation of Wnt signaling pathway
adrenal gland development
inductive cell-cell signaling
somatic stem cell population maintenance
outer ear morphogenesis
embryonic digit morphogenesis
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
embryonic digestive tract development
limb development
olfactory bulb mitral cell layer development
kidney epithelium development
ureteric bud invasion
neural tube closure
negative regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis
transcription from RNA polymerase II promoter
biological_process
olfactory bulb development
neural tube development
forelimb morphogenesis
hindlimb morphogenesis
positive regulation of neuron differentiation
negative regulation of smoothened signaling pathway
mesenchymal stem cell maintenance involved in metanephric nephron morphogenesis
positive regulation of branching involved in ureteric bud morphogenesis
regulation of neural precursor cell proliferation
negative regulation of mesoderm development
negative regulation of ectoderm development
regulation of transcription, DNA-templated
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