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SALL4 Antibody (PA5-29072) in ICC/IF
Immunofluorescence analysis of SALL4 was performed using 70% confluent log phase BeWo cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with SALL4 Polyclonal Antibody (Product # PA5-29072) at 1:500 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A327... View More
Product Details
PA5-29072
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Pig
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 584 and 860 of Human SALL4
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2546548
Product Specific Information
Recommended positive controls: A431.
Predicted reactivity: Pig (82%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Sall4 protein is encoded by the Sall4 gene located on chromosome 20 in humans and belongs to the Spalt-like protein family. It plays an important role in somatic stem cell maintenance and is studied as a stem cell biomarker. It is a zinc finger transcription factor that is upregulated during development and shown to interact with Oct4 and Sox2. It also forms a complex with Nanog to maintain the pluripotency of Embryonic Stem cells (ESCs). Several current research studies focus on its function as an oncogene and tumor biomarker. Some of the reported target genes of Sall4 include p53, Bmi-1, Bcl2 and XIAP.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: dJ1112F19.1; sal-like 4; Sal-like protein 4; spalt-like transcription factor 4; Zinc finger protein 797; Zinc finger protein SALL4
Gene Aliases: 5730441M18Rik; AA407717; AL022809; AW536104; C330011P20Rik; C78083; C78563; DRRS; HSAL4; SALL4; Tex20; ZNF797
UniProt ID: (Human) Q9UJQ4, (Mouse) Q6S7E9
Entrez Gene ID: (Human) 57167, (Pig) 100136902, (Mouse) 99377
negative regulation of transcription from RNA polymerase II promoter
inner cell mass cell proliferation
neural tube closure
ventricular septum development
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
signal transduction
multicellular organism development
embryonic limb morphogenesis
somatic stem cell population maintenance
positive regulation of transcription from RNA polymerase II promoter
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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