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SALL4 Antibody (MA5-50592) in ICC/IF
Immunocytochemistry analysis of SALL4 in NCCIT cells. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were incubated with SALL4 monoclonal antibody (Product # MA5-50592) with a dilution of 1:100 in 2% BSA overnight at 4 . Followed by secondary antibody Goat Anti-Mouse IgG H&L (iFluor™ 488) at a dilution of 1:1,000. PBS instead of the primary antibody was used as the secondary antibody only control. Nucl... View More
Product Details
MA5-50592
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
A7A7
Immunogen
Recombinant protein within human Sall4 aa 903-1053/1053.
Conjugate
Form
Liquid
Concentration
2 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_3091101
Product Specific Information
Positive control: NCCIT cell lysates, NCCIT, human seminoma tissue.
Predicted band size: 112 kDa
Subcellular Location: Cytoplasm, Nucleus.
Target Information
Sall4 protein is encoded by the Sall4 gene located on chromosome 20 in humans and belongs to the Spalt-like protein family. It plays an important role in somatic stem cell maintenance and is studied as a stem cell biomarker. It is a zinc finger transcription factor that is upregulated during development and shown to interact with Oct4 and Sox2. It also forms a complex with Nanog to maintain the pluripotency of Embryonic Stem cells (ESCs). Several current research studies focus on its function as an oncogene and tumor biomarker. Some of the reported target genes of Sall4 include p53, Bmi-1, Bcl2 and XIAP.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: dJ1112F19.1; Sal-like protein 4; spalt-like transcription factor 4; Zinc finger protein 797; Zinc finger protein SALL4
Gene Aliases: DRRS; HSAL4; SALL4; ZNF797
UniProt ID: (Human) Q9UJQ4
Entrez Gene ID: (Human) 57167
negative regulation of transcription from RNA polymerase II promoter
inner cell mass cell proliferation
neural tube closure
ventricular septum development
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
signal transduction
multicellular organism development
embryonic limb morphogenesis
somatic stem cell population maintenance
positive regulation of transcription from RNA polymerase II promoter
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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