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Zeta
SALL4 Recombinant Rabbit Monoclonal Antibody (ZR276), RAbMono™
Product Details
Z2726RT
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Not Applicable
Host/Isotype
Rabbit
/ IgG
Expression System
CHO cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
ZR276
Immunogen
Recombinant fragment (around aa1-185) of human MIC2 protein
Conjugate
Form
Liquid
Concentration
100 µg/mL
Purification
Protein A
Storage buffer
tris with BSA, NP-40
Contains
<0.1% sodium azide
Storage conditions
4° C
Shipping conditions
Wet ice
Product Specific Information
This product is diluted and in a ready-to-use formulation.
A recommended positive control tissue for this product is Seminoma, however positive controls are not limited to this tissue type.
The primary antibody is intended for laboratory professional use in the detection of the corresponding protein in formalin-fixed, paraffin-embedded tissue stained in manual qualitative immunohistochemistry (IHC) testing. This antibody is intended to be used after the primary diagnosis of tumor has been made by conventional histopathology using non-immunological histochemical stains.
Antibody is used with formalin-fixed and paraffin-embedded sections. Pretreatment of deparaffinized tissue with heat-induced epitope retrieval or enzymatic retrieval is recommended. In general, immunohistochemical (IHC) staining techniques allow for the visualization of antigens via the sequential application of a specific antibody to the antigen (primary antibody), a secondary antibody to the primary antibody (link antibody), an enzyme complex and a chromogenic substrate with interposed washing steps. The enzymatic activation of the chromogen results in a visible reaction product at the antigen site. Results are interpreted using a light microscope and aid in the differential diagnosis of pathophysiological processes, which may or may not be associated with a particular antigen.
A positive tissue control must be run with every staining procedure performed. This tissue may contain both positive and negative staining cells or tissue components and serve as both the positive and negative control tissue. External Positive control materials should be fresh autopsy/biopsy/surgical specimens fixed, processed and embedded as soon as possible in the same manner as the patient sample (s). Positive tissue controls are indicative of correctly prepared tissues and proper staining methods. The tissues used for the external positive control materials should be selected from the patient specimens with well-characterized low levels of the positive target activity that gives weak positive staining. The low level of positivity for external positive controls is designed to ensure detection of subtle changes in the primary antibody sensitivity from instability or problems with the staining methodology. A tissue with weak positive staining is more suitable for optimal quality control and for detecting minor levels of reagent degradation.
Internal or external negative control tissue may be used depending on the guidelines and policies that govern the organization to which the end user belongs to. The variety of cell types present in many tissue sections offers internal negative control sites, but this should be verified by the user. The components that do not stain should demonstrate the absence of specific staining, and provide an indication of non-specific background staining. If specific staining occurs in the negative tissue control sites, results with the patient specimens must be considered invalid.
Target Information
Sall4 protein is encoded by the Sall4 gene located on chromosome 20 in humans and belongs to the Spalt-like protein family. It plays an important role in somatic stem cell maintenance and is studied as a stem cell biomarker. It is a zinc finger transcription factor that is upregulated during development and shown to interact with Oct4 and Sox2. It also forms a complex with Nanog to maintain the pluripotency of Embryonic Stem cells (ESCs). Several current research studies focus on its function as an oncogene and tumor biomarker. Some of the reported target genes of Sall4 include p53, Bmi-1, Bcl2 and XIAP.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: dJ1112F19.1; Sal-like protein 4; spalt-like transcription factor 4; Zinc finger protein 797; Zinc finger protein SALL4
Gene Aliases: DRRS; HSAL4; SALL4; ZNF797
UniProt ID: (Human) Q9UJQ4
Entrez Gene ID: (Human) 57167
negative regulation of transcription from RNA polymerase II promoter
inner cell mass cell proliferation
neural tube closure
ventricular septum development
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
signal transduction
multicellular organism development
embryonic limb morphogenesis
somatic stem cell population maintenance
positive regulation of transcription from RNA polymerase II promoter
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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