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Invitrogen
SARS-CoV-2 Spike Protein (RBD) Recombinant Human Monoclonal Antibody (P05DHu), Alexa Fluor™ 647, eBioscience™
FIGURE: 1 / 1
SARS-CoV-2 Spike Protein (RBD) Antibody (51-6490-82) in Flow
HEK 293 cells were transfected with a vector encoding full length spike protein from SARS CoV2 (pink histogram) or left untransfected (blue histogram) and then stained with 0.125 µg of Anti-SARS CoV2 Spike Protein RBD Recombinant Human Monoclonal Antibody, Alexa Fluor 647 (Product # 51-6490-82). Viable cells were used for analysis as determined by Fixable Viability Dye eFluor 520 (Product # 65-0867-18).
Product Details
51-6490-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Virus
Published species
Human,
Non-human primate
Host/Isotype
Human
/ IgG1, kappa
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
P05DHu
Immunogen
This antibody was developed using computationally derived variants of SARS-CoV antibody structures and is shown to bind Spike Protein S1 subunit (RBD) of SARS CoV2
Conjugate
Excitation/Emission Max
650/671 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Protein A
Storage buffer
proprietary buffer/PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Wet ice
RRID
AB_2884044
Product Specific Information
Description: This antibody recognizes the RBD domain of SARS CoV2 Spike protein.
Applications Tested: This P05DHu Alexa Fluor™ 647 conjugate has been tested by flow cytometric analysis of HEK 293 cells transfected with a vector encoding full length spike protein from SARS CoV2. This may be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as 2019-nCoV (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold symptoms to severe effects including death and respiratory distress. The CoV Spike (S) protein plays the most important roles in viral attachment, fusion and entry, and serves as a target for development of antibodies, entry inhibitors and vaccines. Of note is the Spike protein receptor-binding domain (RBD, S-RBD) in SARS-CoV-2 Spike protein which binds strongly to human and bat angiotensin-converting enzyme 2 (ACE2) receptors. SARS-CoV-2 RBD exhibits significantly higher binding affinity to ACE2 receptor than SARS-CoV RBD in comparison. S1 contains a receptor-binding domain (RBD) that can specifically bind to angiotensin-converting enzyme 2 (ACE2), the receptor on target cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Coronavirus; COVID; Covid 19; COVID-19; COVID19; SARS; SARS Coronavirus; SARS CoV; SARS CoV-2; SARS CoV2; SARS Spike Protein; SARS-CoV-2; SARS-CoV2
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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