Invitrogen

SARS Coronavirus Nucleocapsid Polyclonal Antibody

SARS Coronavirus Nucleocapsid Antibody in Western Blot (WB)

FIGURE: 1 / 7

SARS Coronavirus Nucleocapsid Antibody (PA1-41098) in WB

Western blot analysis of SARS Coronavirus Nucleocapsid in (A) untransfected mouse melanoma cell lysate and (B) transfected cell lysate. Samples were incubated in SARS Coronavirus Nucleocapsid polyclonal antibody (Product # PA1-41098).

SARS Coronavirus Nucleocapsid Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

PA1-41098

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:2,000

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-Dependent

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:10-1:500

View 4 publications 4 publications

Product Specifications

Species Reactivity

Virus

Published species

Virus

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues 399-411 of putative SARS nucleocapsid.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1087200

Product Specific Information

The immunogen is 77% ( 10/13) homologous to SARS-COV-2 (COVID-19) Nucleocapsid protein. The cross reactivity is not empirically tested.

Suggested positive control: transfected mouse melanoma cell lysate.

Target Information

Severe Acute Respiratory Syndrome (SARS), an emerging disease characterized by atypical pneumonia, has recently been attributed to a novel coronavirus (SARS-CoV). The nucleocapsid protein (N) was one of the structural proteins of SARS-CoV (N, S, sE, M). The severe acute respiratory syndrome coronavirus (SARS-CoV) nucleocapsid (N) protein is the most abundant protein in the virus-infected cells. The SARS-CoV-2 S gene has 76% amino acid similarity to the SARS-CoV S gene, and nonsynonymous mutations developed in the S protein as the SARS-CoV epidemic developed, reports have shown that the crystal structure of the SARS-CoV-2 nucleocapsid protein is similar to those of previously described coronavirus N proteins, but their surface electrostatic potential characteristics are distinct.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Genome-wide bidirectional CRISPR screens identify mucins as host factors modulating SARS-CoV-2 infection.

Nature genetics

Biering SB,Sarnik SA,Wang E,Zengel JR,Leist SR,Schäfer A,Sathyan V,Hawkins P,Okuda K,Tau C,Jangid AR,Duffy CV,Wei J,Gilmore RC,Alfajaro MM,Strine MS,Nguyenla X,Van Dis E,Catamura C,Yamashiro LH,Belk JA,Begeman A,Stark JC,Shon DJ,Fox DM,Ezzatpour S,Huang E,Olegario N,Rustagi A,Volmer AS,Livraghi-Butrico A,Wehri E,Behringer RR,Cheon DJ,Schaletzky J,Aguilar HC,Puschnik AS,Button B,Pinsky BA,Blish CA,Baric RS,O'Neal WK,Bertozzi CR,Wilen CB,Boucher RC,Carette JE,Stanley SA,Harris E,Konermann S,Hsu PD

PA1-41098 was used in Immunohistochemistry to conclude that this functional landscape of SARS-CoV-2 host factors provides a physiologically relevant starting point for new host-directed therapeutics and highlights airway mucins as a host defense mechanism.

Mon Aug 01 00:00:00 EDT 2022

KIM-1/TIM-1 is a Receptor for SARS-CoV-2 in Lung and Kidney.

medRxiv : the preprint server for health sciences

Mori Y,Fink C,Ichimura T,Sako K,Mori M,Lee NN,Aschauer P,Padmanabha Das KM,Hong S,Song M,Padera RF,Weins A,Lee LP,Nasr ML,Dekaban GA,Dikeakos JD,Bonventre JV

PA1-41098 was used in Immunocytochemistry to show that KIM-1 acts as a receptor for SARS-CoV-2 both in lung and kidney epithelia.

Tue Jan 11 00:00:00 EST 2022

SARS-CoV-2 infection of the oral cavity and saliva.

Nature medicine

Huang N,Pérez P,Kato T,Mikami Y,Okuda K,Gilmore RC,Conde CD,Gasmi B,Stein S,Beach M,Pelayo E,Maldonado JO,Lafont BA,Jang SI,Nasir N,Padilla RJ,Murrah VA,Maile R,Lovell W,Wallet SM,Bowman NM,Meinig SL,Wolfgang MC,Choudhury SN,Novotny M,Aevermann BD,Scheuermann RH,Cannon G,Anderson CW,Lee RE,Marchesan JT,Bush M,Freire M,Kimple AJ,Herr DL,Rabin J,Grazioli A,Das S,French BN,Pranzatelli T,Chiorini JA,Kleiner DE,Pittaluga S,Hewitt SM,Burbelo PD,Chertow D,Frank K,Lee J,Boucher RC,Teichmann SA,Warner BM,Byrd KM

PA1-41098 was used in Immunohistochemistry to show that the oral cavity is an important site for SARS-CoV-2 infection and implicate saliva as a potential route of SARS-CoV-2 transmission.

Sat May 01 00:00:00 EDT 2021

Broad-Spectrum Antiviral Activity of 3D8, a Nucleic Acid-Hydrolyzing Single-Chain Variable Fragment (scFv), Targeting SARS-CoV-2 and Multiple Coronaviruses In Vitro.

Viruses

Lee G,Budhathoki S,Lee GY,Oh KJ,Ham YK,Kim YJ,Lim YR,Hoang PT,Lee Y,Lim SW,Kim JM,Cho S,Kim TH,Song JW,Lee S,Kim WK

Published figure using SARS Coronavirus Nucleocapsid polyclonal antibody (Product # PA1-41098) in Western Blot

Fri Apr 09 00:00:00 EDT 2021

Experimental Susceptibility of North American Raccoons (Procyon lotor) and Striped Skunks (Mephitis mephitis) to SARS-CoV-2.

Frontiers in veterinary science

Francisco R,Hernandez SM,Mead DG,Adcock KG,Burke SC,Nemeth NM,Yabsley MJ

PA1-41098 was used in Immunohistochemistry to show that continued outbreaks in non-domestic species, wild and captive, highlight that additional research on the susceptibility of SARS-CoV-2 in wildlife, especially musteloidea, and of conservation concern, is needed.

Sun Nov 05 00:00:00 EDT 2023

A Mouse-Adapted SARS-CoV-2 Induces Acute Lung Injury and Mortality in Standard Laboratory Mice.

Cell

Leist SR,Dinnon KH,Schäfer A,Tse LV,Okuda K,Hou YJ,West A,Edwards CE,Sanders W,Fritch EJ,Gully KL,Scobey T,Brown AJ,Sheahan TP,Moorman NJ,Boucher RC,Gralinski LE,Montgomery SA,Baric RS

PA1-41098 was used in Immunohistochemistry to generate a new mouse-adapted SARS-CoV-2 virus that induces aspects of severe COVID-19 disease, such as acute lung injury and mortality, in standard laboratory mice.

Thu Nov 12 00:00:00 EST 2020

SARS-CoV-2 Reverse Genetics Reveals a Variable Infection Gradient in the Respiratory Tract.

Cell

Hou YJ,Okuda K,Edwards CE,Martinez DR,Asakura T,Dinnon KH,Kato T,Lee RE,Yount BL,Mascenik TM,Chen G,Olivier KN,Ghio A,Tse LV,Leist SR,Gralinski LE,Schäfer A,Dang H,Gilmore R,Nakano S,Sun L,Fulcher ML,Livraghi-Butrico A,Nicely NI,Cameron M,Cameron C,Kelvin DJ,de Silva A,Margolis DM,Markmann A,Bartelt L,Zumwalt R,Martinez FJ,Salvatore SP,Borczuk A,Tata PR,Sontake V,Kimple A,Jaspers I,O'Neal WK,Randell SH,Boucher RC,Baric RS

PA1-41098 was used in Immunocytochemistry-immunoflourescence to highlight the nasal susceptibility to SARS-CoV-2 with likely subsequent aspiration-mediated virus seeding to the lung in SARS-CoV-2 pathogenesis.

Thu Jul 23 00:00:00 EDT 2020

Human Lung Stem Cell-Based Alveolospheres Provide Insights into SARS-CoV-2-Mediated Interferon Responses and Pneumocyte Dysfunction.

Cell stem cell

Katsura H,Sontake V,Tata A,Kobayashi Y,Edwards CE,Heaton BE,Konkimalla A,Asakura T,Mikami Y,Fritch EJ,Lee PJ,Heaton NS,Boucher RC,Randell SH,Baric RS,Tata PR

Published figure using SARS Coronavirus Nucleocapsid polyclonal antibody (Product # PA1-41098) in Immunohistochemistry

Thu Dec 03 00:00:00 EST 2020

Human Lung Stem Cell-Based Alveolospheres Provide Insights into SARS-CoV-2-Mediated Interferon Responses and Pneumocyte Dysfunction.

Cell stem cell

Katsura H,Sontake V,Tata A,Kobayashi Y,Edwards CE,Heaton BE,Konkimalla A,Asakura T,Mikami Y,Fritch EJ,Lee PJ,Heaton NS,Boucher RC,Randell SH,Baric RS,Tata PR

Published figure using SARS Coronavirus Nucleocapsid polyclonal antibody (Product # PA1-41098) in Immunohistochemistry

Thu Dec 03 00:00:00 EST 2020

Unspecific post-mortem findings despite multiorgan viral spread in COVID-19 patients.

Critical care (London, England)

Remmelink M,De Mendonça R,D'Haene N,De Clercq S,Verocq C,Lebrun L,Lavis P,Racu ML,Trépant AL,Maris C,Rorive S,Goffard JC,De Witte O,Peluso L,Vincent JL,Decaestecker C,Taccone FS,Salmon I

PA1-41098 was used in Immunohistochemistry to evaluate whether some specific post-mortem features are observed in these patients and if these changes are related to the presence of the virus in different organs.

Wed Aug 12 00:00:00 EDT 2020

Unspecific post-mortem findings despite multiorgan viral spread in COVID-19 patients.

Critical care (London, England)

Remmelink M,De Mendonça R,D'Haene N,De Clercq S,Verocq C,Lebrun L,Lavis P,Racu ML,Trépant AL,Maris C,Rorive S,Goffard JC,De Witte O,Peluso L,Vincent JL,Decaestecker C,Taccone FS,Salmon I

Wed Aug 12 00:00:00 EDT 2020

SARS-CoV-2 D614G Variant Exhibits Enhanced Replication ex vivo and Earlier Transmission in vivo.

bioRxiv : the preprint server for biology

Hou YJ,Chiba S,Halfmann P,Ehre C,Kuroda M,Dinnon KH,Leist SR,Schäfer A,Nakajima N,Takahashi K,Lee RE,Mascenik TM,Edwards CE,Tse LV,Boucher RC,Randell SH,Suzuki T,Gralinski LE,Kawaoka Y,Baric RS

PA1-41098 was used in Immunocytochemistry to demonstrate the SARS-CoV2 D614G substitution enhances infectivity, replication fitness, and early transmission.

Tue Sep 29 00:00:00 EDT 2020

SARS-CoV-2 D614G Variant Exhibits Enhanced Replication ex vivo and Earlier Transmission in vivo.

bioRxiv : the preprint server for biology

Hou YJ,Chiba S,Halfmann P,Ehre C,Kuroda M,Dinnon KH,Leist SR,Schäfer A,Nakajima N,Takahashi K,Lee RE,Mascenik TM,Edwards CE,Tse LV,Boucher RC,Randell SH,Suzuki T,Gralinski LE,Kawaoka Y,Baric RS

PA1-41098 was used in Immunocytochemistry to demonstrate the SARS-CoV2 D614G substitution enhances infectivity, replication fitness, and early transmission.

Tue Sep 29 00:00:00 EDT 2020

Integrated Single-Cell Atlases Reveal an Oral SARS-CoV-2 Infection and Transmission Axis.

medRxiv : the preprint server for health sciences

Huang N,Perez P,Kato T,Mikami Y,Okuda K,Gilmore RC,Domínguez Conde C,Gasmi B,Stein S,Beach M,Pelayo E,Maldonado J,LaFont B,Padilla R,Murrah V,Maile R,Lovell W,Wallet S,Bowman NM,Meinig SL,Wolfgang MC,Choudhury SN,Novotny M,Aevermann BD,Scheuermann R,Cannon G,Anderson C,Marchesan J,Bush M,Freire M,Kimple A,Herr DL,Rabin J,Grazioli A,French BN,Pranzatelli T,Chiorini JA,Kleiner DE,Pittaluga S,Hewitt S,Burbelo PD,Chertow D,Frank K,Lee J,Boucher RC,Teichmann SA,Warner BM,Byrd KM

PA1-41098 was used in Immunocytochemistry to investigate the involvement of the oral cavity in COVID-19.

Tue Oct 27 00:00:00 EDT 2020

Bioinformatics

Protein Aliases: Coronavirus; COVID; COVID 19; COVID-19; COVID19; SARS; SARS Coronavirus; SARS CoV; SARS CoV-2; SARS CoV2; SARS Nucleocapsid; SARS-CoV-2; SARS-CoV2

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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SARS Coronavirus Nucleocapsid Polyclonal Antibody

SARS Coronavirus Nucleocapsid Antibody (PA1-41098) in WB

Product Details

PA1-41098

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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