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Invitrogen
SARS Coronavirus Spike Protein Polyclonal Antibody
FIGURE: 1 / 1
SARS Coronavirus Spike Protein Antibody (PA5-120761) in WB
Western blot analysis of SARS Coronavirus Spike Protein in extracts of various cell lines. Samples were incubated with SARS Coronavirus Spike Protein Polyclonal antibody (Product # PA5-120761) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time... View More
Product Details
PA5-120761
Product Specifications
Species Reactivity
Virus
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 600-700 of coronavirus Spike (NP_8288511)
Conjugate
Form
Liquid
Concentration
1.04 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2914333
Product Specific Information
Positive test controls include: 293T.
Immunogen sequence: DVNCTDVSTA IHADQLTPAW RIYSTGNNVF QTQAGCLIGA EHVDTSYECD IPIGAGICAS YHTVSLLRST SQKSIVAYTM SLGADSSIAY SNNTIAIPTN F
Target Information
It has recently been shown that SARS (severe acute respiratory syndrome) is caused by a human coronavirus. Human coronaviruses are the major cause of upper respiratory tract illness, such as the common cold, in humans. Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The first step in coronavirus infection is binding of the viral spike protein, a 139-kDa protein, to certain receptors on host cells. The spike protein is the main surface antigen of the coronavirus. The glycosylated spike protein (as well as the nucleocapsid protein) can be detected in infected cell culture supernatants with antisera from SARS patients.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Coronavirus; COVID; Covid 19; COVID-19; COVID19; SARS; SARS Coronavirus; SARS CoV; SARS CoV-2; SARS CoV2; SARS Spike Protein; SARS-CoV-2; SARS-CoV2
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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