Invitrogen

SARS/SARS-CoV-2 Spike Protein S2 Monoclonal Antibody (1A9)

4 Published Figures

10 References

View all (18) SARS-CoV-2 Spike Protein S2 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (44)
Published Figures (4)

SARS/SARS-CoV-2 Spike Protein S2 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 48

SARS/SARS-CoV-2 Spike Protein S2 Antibody (MA5-35946) in ICC/IF

SARS-CoV/SARS-CoV-2 (COVID-19) spike antibody [1A9] detects SARS-CoV-2 by immunofluorescent analysis. Sample: Vero E6 cells were infected with SARS-CoV-2 (MOI of 1) and fixed with chilled methanol/acetone. Green: SARS-CoV-2 infected Vero E6 cells stained by SARS-CoV/SARS-CoV-2 (COVID-19) spike antibody [1A9] (Product # MA5-35946). Blue: Nuclei were counterstained with DAPI. *From Zheng Z, et al. bioRxiv (20... View More

SARS/SARS-CoV-2 Spike Protein S2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

SARS/SARS-CoV-2 Spike Protein S2 Antibody in Western Blot (WB)

SARS/SARS-CoV-2 Spike Protein S2 Antibody in Flow Cytometry (Flow)

SARS/SARS-CoV-2 Spike Protein S2 Antibody in Immunoprecipitation (IP)

SARS/SARS-CoV-2 Spike Protein S2 Antibody in ELISA (ELISA)

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Product Details

MA5-35946

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 5 publications 5 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:500

Immunocytochemistry (ICC/IF)

1:100-1:2,000

View 2 publications 2 publications

Flow Cytometry (Flow)

Assay-dependent

ELISA (ELISA)

Assay-dependent

Immunoprecipitation (IP)

Assay-dependent

Neutralization (Neu)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Virus

Published species

Human, Virus

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

1A9

Immunogen

SARS-CoV SDelta10 (within S2 domain) protein (1029-1192 a.a.)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2866558

Target Information

SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as 2019-nCoV (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold symptoms to severe effects including death and respiratory distress. SARS-CoV-2 Spike Protein is composed of S1 domain and S2 domain (Spike Protein). The S2 domain plays a role in fusion. Further, S proteins plays an important role in the induction of neutralizing-antibodies and T-cell responses, as well as protective immunity. CoV diversity is reflected in the variable Spike proteins (S proteins), which have evolved into forms differing in their receptor interactions and their response to various environmental triggers of virus-cell membrane fusion.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

SARS-CoV-2 protein ORF8 limits expression levels of Spike antigen and facilitates immune evasion of infected host cells.

The Journal of biological chemistry

Kim IJ,Lee YH,Khalid MM,Chen IP,Zhang Y,Ott M,Verdin E

Published figure using SARS/SARS-CoV-2 Spike Protein S2 monoclonal antibody (Product # MA5-35946) in Immunocytochemistry

Tue Aug 01 00:00:00 EDT 2023

Design and preclinical evaluation of a universal SARS-CoV-2 mRNA vaccine.

Frontiers in immunology

Qin J,Jeon JH,Xu J,Langston LK,Marasini R,Mou S,Montoya B,Melo-Silva CR,Jeon HJ,Zhu T,Sigal LJ,Xu R,Zhu H

MA5-35946 was used in Western Blotting to demonstrate that induction of T cell responses, in the absence of preexisting antibodies, is sufficient to confer protection against severe disease, and that a vaccine containing mRNAs encoding both the Spike and MTE could be further developed as a universal SARS-CoV-2 vaccine.

Tue Apr 11 00:00:00 EDT 2023

Mutations in SARS-CoV-2 variants of concern link to increased spike cleavage and virus transmission.

Cell host & microbe

Escalera A,Gonzalez-Reiche AS,Aslam S,Mena I,Laporte M,Pearl RL,Fossati A,Rathnasinghe R,Alshammary H,van de Guchte A,Farrugia K,Qin Y,Bouhaddou M,Kehrer T,Zuliani-Alvarez L,Meekins DA,Balaraman V,McDowell C,Richt JA,Bajic G,Sordillo EM,Dejosez M,Zwaka TP,Krogan NJ,Simon V,Albrecht RA,van Bakel H,García-Sastre A,Aydillo T

MA5-35946 was used in Western Blot to characterize emerging SARS-CoV-2 spike polymorphisms in vitro and in vivo to understand their impact on transmissibility and virus pathogenicity and fitness.

Wed Mar 09 00:00:00 EST 2022

Zonulin Antagonist, Larazotide (AT1001), As an Adjuvant Treatment for Multisystem Inflammatory Syndrome in Children: A Case Series.

Critical care explorations

Yonker LM,Swank Z,Gilboa T,Senussi Y,Kenyon V,Papadakis L,Boribong BP,Carroll RW,Walt DR,Fasano A

MA5-35946 was used in Neutralization to suggest that expansion of clinical trials is urgently needed to ascertain the clinical impact of larazotide on MIS-C.

Tue Feb 01 00:00:00 EST 2022

Development of a hybrid alphavirus-SARS-CoV-2 pseudovirion for rapid quantification of neutralization antibodies and antiviral drugs.

Cell reports methods

Hetrick B,Chilin LD,He S,Dabbagh D,Alem F,Narayanan A,Luchini A,Li T,Liu X,Copeland J,Pak A,Cunningham T,Liotta L,Petricoin EF,Andalibi A,Wu Y

MA5-35946 was used in Miscellaneous, ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to demonstrate that Ha-CoV-2 can be used as a robust platform for the rapid quantification of neutralizing antibodies against SARS-CoV-2 and its emerging variants.

Mon Mar 28 00:00:00 EDT 2022

Protocol to isolate and assess spike protein cleavage in SARS-CoV-2 variants obtained from clinical COVID-19 samples.

STAR protocols

Escalera A,García-Sastre A,Aydillo T

MA5-35946 was used in Western Blotting to present a comprehensive protocol to assess SARS-CoV-2 spike protein cleavage in viral supernatants from SARS-CoV-2-infected cells.

Fri Sep 16 00:00:00 EDT 2022

Multiple sites on SARS-CoV-2 spike protein are susceptible to proteolysis by cathepsins B, K, L, S, and V.

Protein science : a publication of the Protein Society

Bollavaram K,Leeman TH,Lee MW,Kulkarni A,Upshaw SG,Yang J,Song H,Platt MO

MA5-35946 was used in Western Blot to highlight cathepsins B, K, L, S, and V for consideration in SARS-CoV-2 infection and presents methodologies by which other proteases can be screened to determine a role in viral entry.

Tue Jun 01 00:00:00 EDT 2021

Immunogenicity and efficacy of mRNA COVID-19 vaccine MRT5500 in preclinical animal models.

NPJ vaccines

Kalnin KV,Plitnik T,Kishko M,Zhang J,Zhang D,Beauvais A,Anosova NG,Tibbitts T,DiNapoli J,Ulinski G,Piepenhagen P,Cummings SM,Bangari DS,Ryan S,Huang PD,Huleatt J,Vincent D,Fries K,Karve S,Goldman R,Gopani H,Dias A,Tran K,Zacharia M,Gu X,Boeglin L,Abysalh J,Vargas J,Beaulieu A,Shah M,Jeannotte T,Gillis K,Chivukula S,Swearingen R,Landolfi V,Fu TM,DeRosa F,Casimiro D

Published figure using SARS/SARS-CoV-2 Spike Protein S2 monoclonal antibody (Product # MA5-35946) in Immunocytochemistry

Mon Apr 19 00:00:00 EDT 2021

SARS-CoV-2 Spike Targets USP33-IRF9 Axis via Exosomal miR-148a to Activate Human Microglia.

Frontiers in immunology

Mishra R,Banerjea AC

MA5-35946 was used in Western Blot to provide novel and relevant insights regarding the impact of Spike gene on shuttling of host microRNAs via exosomes to trigger the neuroinflammation.

Fri May 14 00:00:00 EDT 2021

SARS-CoV-2 Spike Targets USP33-IRF9 Axis via Exosomal miR-148a to Activate Human Microglia.

Frontiers in immunology

Mishra R,Banerjea AC

MA5-35946 was used in Western Blot to provide novel and relevant insights regarding the impact of Spike gene on shuttling of host microRNAs via exosomes to trigger the neuroinflammation.

Fri May 14 00:00:00 EDT 2021

SARS-CoV-2 colonization of maternal and fetal cells of the human placenta promotes alteration of local renin-angiotensin system.

Med (New York, N.Y.)

Verma S,Joshi CS,Silverstein RB,He M,Carter EB,Mysorekar IU

MA5-35946 was used in Immunohistochemistry to investigate the pathophysiology of SARS-CoV-2 at maternal-fetal interface in pregnant women who tested positive for the virus using RNA in situ hybridization (viral RNA), immunohistochemistry, and hematoxylin and eosin staining.

Fri May 14 00:00:00 EDT 2021

Bioinformatics

Protein Aliases: Coronavirus; COVID; Covid 19; COVID-19; COVID-19 S2; COVID19; COVID19 Spike S2; SARS; SARS Coronavirus; SARS CoV; SARS CoV-2; SARS CoV2; SARS Spike Protein; SARS-CoV-2; SARS-CoV2

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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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SARS/SARS-CoV-2 Spike Protein S2 Monoclonal Antibody (1A9)

SARS/SARS-CoV-2 Spike Protein S2 Antibody (MA5-35946) in ICC/IF

Product Details

MA5-35946

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Immunoprecipitation (IP)

Neutralization (Neu)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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