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SCF Antibody (500-P48G-1MG) in WB
Western Blot: To detect hSCF by Western Blot analysis SCF Polyclonal Antibody (Product # 500-P48G-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hSCF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P48G-1MG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Goat
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human SCF
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2930576
Product Specific Information
AA Sequence of recombinant protein: MEGICRNRVT NNVKDVTKLV ANLPKDYMIT LKYVPGMDVL PSHCWISEMV VQLSDSLTDL LDKFSNISEG LSNYSIIDKL VNIVDDLVEC VKENSSKDLK KSFKSPEPRL FTPEEFFRIF NRSIDAFKDF VVASETSDCV VSSTLSPEKD SRVSVTKPFM LPPVA.
Preparation: Produced from sera of goats immunized with highly pure Recombinant Human SCF. Anti-Human SCF-specific antibody was purified by affinity chromatography employing an immobilized Human SCF matrix.
Sandwich ELISA: To detect Human SCF by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human SCF (500-P48GBt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human SCF.
Western Blot: To detect hSCF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hSCF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
Stem cell factor (SCF) is the ligand of the c-Kit oncogene and is expressed by various structural and inflammatory cells in the airways. Binding of SCF by the c-Kit receptor leads to homodimerization of the receptor and the activation of signalling pathways such as PI-3, PLC-gamma, Jak/STAT, and MAP kinase pathways. SCF expression leads to the induction of mast cell survival and the expression and release of histamine, pro-inflammatory cytokines and chemokines. The inhibition of the SCF/c-Kit pathway leads to a decrease in histamine levels, mast cell and eosinophil infiltration, IL-4 production and airway hyperresponsiveness, suggesting this pathway may be a useful therapeutic target in inflammatory diseases such as asthma. At least two isoforms of SCF are known to exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: c-Kit ligand; DKFZp686F2250; familial progressive hyperpigmentation 2; H-SCF; Kit ligand; kit-ligand; M-SCF; Mast cell growth factor; MGF; SCF; steel factor; Stem cell factor
Gene Aliases: DCUA; DFNA69; FPH2; FPHH; Kitl; KITLG; KL-1; MGF; SCF; SF; SHEP7
UniProt ID: (Human) P21583
Entrez Gene ID: (Human) 4254
MAPK cascade
ovarian follicle development
neural crest cell migration
positive regulation of leukocyte migration
positive regulation of myeloid leukocyte differentiation
cell adhesion
signal transduction
cell proliferation
male gonad development
regulation of phosphatidylinositol 3-kinase signaling
negative regulation of mast cell apoptotic process
embryonic hemopoiesis
ectopic germ cell programmed cell death
positive regulation of MAP kinase activity
positive regulation of GTPase activity
positive regulation of melanocyte differentiation
positive regulation of DNA replication
positive regulation of Ras protein signal transduction
phosphatidylinositol phosphorylation
phosphatidylinositol-mediated signaling
positive regulation of peptidyl-tyrosine phosphorylation
positive regulation of mast cell proliferation
extrinsic apoptotic signaling pathway in absence of ligand
positive regulation of hematopoietic stem cell proliferation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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