Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 1
SCF Antibody (PA5-47637) in Neu
Neutralization of SCF in TF‚1 human erythroleukemic cell line. Samples were incubated in SCF polyclonal antibody (Product # PA5-47637). Recombinant MouseSCF/c‚kit Ligand stimulates proliferation in the TF‚1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant MouseSCF/c‚kit Ligand (25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-MouseSCF/c-kit Ligand Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 0.3‚0.6 µg/mL.
Product Details
PA5-47637
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived recombinant mouse SCF/c-kit Ligand Lys26-Ala189
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2609222
Product Specific Information
In Western blots, less than 20% cross-reactivity with recombinant human SCF is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.
Target Information
Stem cell factor (SCF) is the ligand of the c-Kit oncogene and is expressed by various structural and inflammatory cells in the airways. Binding of SCF by the c-Kit receptor leads to homodimerization of the receptor and the activation of signalling pathways such as PI-3, PLC-gamma, Jak/STAT, and MAP kinase pathways. SCF expression leads to the induction of mast cell survival and the expression and release of histamine, pro-inflammatory cytokines and chemokines. The inhibition of the SCF/c-Kit pathway leads to a decrease in histamine levels, mast cell and eosinophil infiltration, IL-4 production and airway hyperresponsiveness, suggesting this pathway may be a useful therapeutic target in inflammatory diseases such as asthma. At least two isoforms of SCF are known to exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: C-kit ligand; cloud gray; DKFZp686F2250; grizzle-belly; H-SCF; Hematopoietic growth factor KL; Kit ligand; kit-ligand; M-SCF; Mast cell growth factor; MGF; SCF; Steel factor; Stem cell factor
Gene Aliases: blz; Clo; Con; contrasted; Gb; Kitl; Kitlg; Mgf; SCF; SF; Sl; SLF
UniProt ID: (Mouse) P20826
Entrez Gene ID: (Mouse) 17311
ovarian follicle development
neural crest cell migration
positive regulation of leukocyte migration
positive regulation of myeloid leukocyte differentiation
cell adhesion
germ cell development
positive regulation of cell proliferation
negative regulation of mast cell apoptotic process
embryonic hemopoiesis
ectopic germ cell programmed cell death
negative regulation of apoptotic process
positive regulation of MAP kinase activity
positive regulation of melanocyte differentiation
positive regulation of DNA replication
positive regulation of Ras protein signal transduction
positive regulation of peptidyl-tyrosine phosphorylation
positive regulation of mast cell proliferation
extrinsic apoptotic signaling pathway in absence of ligand
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support