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Invitrogen
SEMA4A Monoclonal Antibody (KL-1 (aka SK31)), eBioscience™
Product Details
14-1002-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Rat
Host/Isotype
Rat
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
KL-1 (aka SK31)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10547286
Product Specific Information
Description: The KL-1 (aka SK31) antibody reacts with mouse Sema4A, a class IV semaphorin originally identified as SemB. Sema4A is structurally similar to Sema4D/CD100. Sema4A is reported to be expressed by dendritic cells, B cells and activated T cells. Although published data suggested that Sema4A binds to Tim-2, a T cell surface receptor, it remains to be definitively shown that these two molecules have a receptor/ligand relation. It is suggested that Sema4a can provide costimulatory signals for T cells.
This KL-1 antibody works in immunoblotting but requires non-reducing conditions.
Applications Reported: This KL-1 (aka SK31) antibody has been reported for use in immunoblotting (WB).
Applications Tested: The KL-1 (aka SK31) antibody has been tested by immunoblot analysis of mouse Sema4A transfected cells under nonreducing conditions. This can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
SEMA4A is a member of the semaphorin family of soluble and transmembrane proteins. Semaphorins are involved in guidance of axonal migration during neuronal development and in immune responses.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Sema B; semaphorin 4A; Semaphorin-4A; Semaphorin-B
Gene Aliases: AI132332; Sema4a; Semab; SemB
UniProt ID: (Mouse) Q62178
Entrez Gene ID: (Mouse) 20351
angiogenesis
T cell differentiation involved in immune response
immune system process
multicellular organism development
nervous system development
axonogenesis
regulation of cell shape
regulation of endothelial cell migration
negative regulation of angiogenesis
cell differentiation
T-helper 1 cell differentiation
semaphorin-plexin signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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