Proteintech

SEPT2 Polyclonal Antibody

SEPT2 Antibody in Immunocytochemistry (ICC/IF)

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SEPT2 Antibody (11397-1-AP) in ICC/IF

IF result from Dr. Kevin Corbit. SEPT2 (11397-1-AP) marks the transition zone and cilium of Human hTERT-RPE1 cells and Mouse embryonic fibroblasts.

SEPT2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

SEPT2 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

11397-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:4,000

View 15 publications 15 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:20-1:200

View 11 publications 11 publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Not applicable (N/A)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

SEPT2 Fusion Protein Ag1946 (1-361 aa encoded by BC014455)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.18 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MSKQQPTQF INPETPGYVG FANLPNQVHR KSVKKGFEFT LMVVGESGLG KSTLINSLFL TDLYPERVIS GAAEKIERTV QIEASTVEIE ERGVKLRLTV VDTPGYGDAI NCRDCFKTII SYIDEQFERY LHDESGLNRR HIIDNRVHCC FYFISPFGHG LKPLDVAFMK AIHNKVNIVP VIAKADTLTL KERERLKKRI LDEIEEHNIK IYHLPDAESD EDEDFKEQTR LLKASIPFSV VGSNQLIEAK GKKVRGRLYP WGVVEVENPE HNDFLKLRTM LITHMQDLQE VTQDLHYENF RSERLKRGGR KVENEDMNKD QILLEKEAEL RRMQEMIARM QAQMQMQMQG GDGDGGALGH HV (1-361 aa encoded by BC014455)

Target Information

Septin 2 is a filament-forming cytoskeletal GTPase. Septin 2 is required for normal organization of the actin cytoskeleton and plays a role in the biogenesis of polarized columnar-shaped epithelium by maintaining polyglutamylated microtubules, thus facilitating efficient vesicle transport, and by impeding MAP4 binding to tubulin. It is required for the progression through mitosis, and it forms a scaffold at the midplane of the mitotic splindle required to maintain CENPE localization at kinetochores and consequently chromosome congression. During anaphase, Septin 2 may be required for chromosome segregation and spindle elongation. Septin 2 also plays a role in ciliogenesis and collective cell movements.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Targeted inactivation of the Septin2 and Septin9 genes in myelinating Schwann cells of mice.

Cytoskeleton (Hoboken, N.J.)

Martens AK,Erwig M,Patzig J,Fledrich R,Füchtbauer EM,Werner HB

Tue Sep 19 00:00:00 EDT 2023

Targeted inactivation of the Septin2 and Septin9 genes in myelinating Schwann cells of mice.

Cytoskeleton (Hoboken, N.J.)

Martens AK,Erwig M,Patzig J,Fledrich R,Füchtbauer EM,Werner HB

Tue Sep 19 00:00:00 EDT 2023

An anillin-CIN85-SEPT9 complex promotes intercellular bridge maturation required for successful cytokinesis.

Cell reports

Panagiotou TC,Chen A,Wilde A

Tue Aug 30 00:00:00 EDT 2022

JMJD2C-mediated long non-coding RNA MALAT1/microRNA-503-5p/SEPT2 axis worsens non-small cell lung cancer.

Cell death & disease

Zhang J,Wang M,Wang J,Wang W

Wed Jan 19 00:00:00 EST 2022

Pinch2 regulates myelination in the mouse central nervous system.

Development (Cambridge, England)

Paes de Faria J,Vale-Silva RS,Fässler R,Werner HB,Relvas JB

Fri Jul 01 00:00:00 EDT 2022

Revised subunit order of mammalian septin complexes explains their in vitro polymerization properties.

Molecular biology of the cell

Soroor F,Kim MS,Palander O,Balachandran Y,Collins RF,Benlekbir S,Rubinstein JL,Trimble WS

Mon Feb 01 00:00:00 EST 2021

MicroRNA‑744‑5p is downregulated in colorectal cancer and targets SEPT2 to suppress the malignant phenotype.

Molecular medicine reports

Zhang W,Liao K,Liu D

Fri Jan 01 00:00:00 EST 2021

An endometrial organoid model of interactions between Chlamydia and epithelial and immune cells.

Journal of cell science

Dolat L,Valdivia RH

Mon Mar 08 00:00:00 EST 2021

Rpgrip1l controls ciliary gating by ensuring the proper amount of Cep290 at the vertebrate transition zone.

Molecular biology of the cell

Wiegering A,Dildrop R,Vesque C,Khanna H,Schneider-Maunoury S,Gerhardt C

Thu Apr 15 00:00:00 EDT 2021

The septin cytoskeleton regulates natural killer cell lytic granule release.

The Journal of cell biology

Phatarpekar PV,Overlee BL,Leehan A,Wilton KM,Ham H,Billadeau DD

Mon Nov 02 00:00:00 EST 2020

The septin cytoskeleton regulates natural killer cell lytic granule release.

The Journal of cell biology

Phatarpekar PV,Overlee BL,Leehan A,Wilton KM,Ham H,Billadeau DD

Mon Nov 02 00:00:00 EST 2020

The SEPT12 complex is required for the establishment of a functional sperm head-tail junction.

Molecular human reproduction

Shen YR,Wang HY,Tsai YC,Kuo YC,Wu SR,Wang CY,Kuo PL

Mon Jun 01 00:00:00 EDT 2020

The SEPT12 complex is required for the establishment of a functional sperm head-tail junction.

Molecular human reproduction

Shen YR,Wang HY,Tsai YC,Kuo YC,Wu SR,Wang CY,Kuo PL

Mon Jun 01 00:00:00 EDT 2020

A Septin Cytoskeleton-Targeting Small Molecule, Forchlorfenuron, Inhibits Epithelial Migration via Septin-Independent Perturbation of Cellular Signaling.

Cells

Sun L,Cao X,Lechuga S,Feygin A,Naydenov NG,Ivanov AI

Sun Dec 29 00:00:00 EST 2019

Anillin facilitates septin assembly to prevent pathological outfoldings of central nervous system myelin.

eLife

Erwig MS,Patzig J,Steyer AM,Dibaj P,Heilmann M,Heilmann I,Jung RB,Kusch K,Möbius W,Jahn O,Nave KA,Werner HB

Wed Jan 23 00:00:00 EST 2019

Septin-regulated actin dynamics promote Salmonella invasion of host cells.

Cellular microbiology

Boddy KC,Gao AD,Truong D,Kim MS,Froese CD,Trimble WS,Brumell JH

Mon Oct 01 00:00:00 EDT 2018

Septin-regulated actin dynamics promote Salmonella invasion of host cells.

Cellular microbiology

Boddy KC,Gao AD,Truong D,Kim MS,Froese CD,Trimble WS,Brumell JH

Mon Oct 01 00:00:00 EDT 2018

SEPT12 phosphorylation results in loss of the septin ring/sperm annulus, defective sperm motility and poor male fertility.

PLoS genetics

Shen YR,Wang HY,Kuo YC,Shih SC,Hsu CH,Chen YR,Wu SR,Wang CY,Kuo PL

Wed Mar 01 00:00:00 EST 2017

SEPT12 phosphorylation results in loss of the septin ring/sperm annulus, defective sperm motility and poor male fertility.

PLoS genetics

Shen YR,Wang HY,Kuo YC,Shih SC,Hsu CH,Chen YR,Wu SR,Wang CY,Kuo PL

Wed Mar 01 00:00:00 EST 2017

Wiskott-Aldrich syndrome protein regulates autophagy and inflammasome activity in innate immune cells.

Nature communications

Lee PP,Lobato-Márquez D,Pramanik N,Sirianni A,Daza-Cajigal V,Rivers E,Cavazza A,Bouma G,Moulding D,Hultenby K,Westerberg LS,Hollinshead M,Lau YL,Burns SO,Mostowy S,Bajaj-Elliott M,Thrasher AJ

Fri Nov 17 00:00:00 EST 2017

Wiskott-Aldrich syndrome protein regulates autophagy and inflammasome activity in innate immune cells.

Nature communications

Lee PP,Lobato-Márquez D,Pramanik N,Sirianni A,Daza-Cajigal V,Rivers E,Cavazza A,Bouma G,Moulding D,Hultenby K,Westerberg LS,Hollinshead M,Lau YL,Burns SO,Mostowy S,Bajaj-Elliott M,Thrasher AJ

Fri Nov 17 00:00:00 EST 2017

The requirement of SEPT2 and SEPT7 for migration and invasion in human breast cancer via MEK/ERK activation.

Oncotarget

Zhang N,Liu L,Fan N,Zhang Q,Wang W,Zheng M,Ma L,Li Y,Shi L

Tue Sep 20 00:00:00 EDT 2016

Septin/anillin filaments scaffold central nervous system myelin to accelerate nerve conduction.

eLife

Patzig J,Erwig MS,Tenzer S,Kusch K,Dibaj P,Möbius W,Goebbels S,Schaeren-Wiemers N,Nave KA,Werner HB

Tue Aug 09 00:00:00 EDT 2016

Septin/anillin filaments scaffold central nervous system myelin to accelerate nerve conduction.

eLife

Patzig J,Erwig MS,Tenzer S,Kusch K,Dibaj P,Möbius W,Goebbels S,Schaeren-Wiemers N,Nave KA,Werner HB

Tue Aug 09 00:00:00 EDT 2016

SEPT12 orchestrates the formation of mammalian sperm annulus by organizing core octameric complexes with other SEPT proteins.

Journal of cell science

Kuo YC,Shen YR,Chen HI,Lin YH,Wang YY,Chen YR,Wang CY,Kuo PL

Sun Mar 01 00:00:00 EST 2015

SEPT12 orchestrates the formation of mammalian sperm annulus by organizing core octameric complexes with other SEPT proteins.

Journal of cell science

Kuo YC,Shen YR,Chen HI,Lin YH,Wang YY,Chen YR,Wang CY,Kuo PL

Sun Mar 01 00:00:00 EST 2015

SEPT12 orchestrates the formation of mammalian sperm annulus by organizing core octameric complexes with other SEPT proteins.

Journal of cell science

Kuo YC,Shen YR,Chen HI,Lin YH,Wang YY,Chen YR,Wang CY,Kuo PL

Sun Mar 01 00:00:00 EST 2015

SEPT12 orchestrates the formation of mammalian sperm annulus by organizing core octameric complexes with other SEPT proteins.

Journal of cell science

Kuo YC,Shen YR,Chen HI,Lin YH,Wang YY,Chen YR,Wang CY,Kuo PL

Sun Mar 01 00:00:00 EST 2015

FHL2-driven molecular network mediated Septin2 knockdown inducing apoptosis in mesangial cell.

Proteomics

Lu Y,Cai G,Cui S,Geng W,Chen D,Wen J,Zhang Y,Zhang F,Xie Y,Fu B,Chen X

Sat Nov 01 00:00:00 EDT 2014

FHL2-driven molecular network mediated Septin2 knockdown inducing apoptosis in mesangial cell.

Proteomics

Lu Y,Cai G,Cui S,Geng W,Chen D,Wen J,Zhang Y,Zhang F,Xie Y,Fu B,Chen X

Sat Nov 01 00:00:00 EDT 2014

FHL2-driven molecular network mediated Septin2 knockdown inducing apoptosis in mesangial cell.

Proteomics

Lu Y,Cai G,Cui S,Geng W,Chen D,Wen J,Zhang Y,Zhang F,Xie Y,Fu B,Chen X

Sat Nov 01 00:00:00 EDT 2014

FHL2-driven molecular network mediated Septin2 knockdown inducing apoptosis in mesangial cell.

Proteomics

Lu Y,Cai G,Cui S,Geng W,Chen D,Wen J,Zhang Y,Zhang F,Xie Y,Fu B,Chen X

Sat Nov 01 00:00:00 EDT 2014

Septins arrange F-actin-containing fibers on the Chlamydia trachomatis inclusion and are required for normal release of the inclusion by extrusion.

mBio