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SF1 Antibody (A303-214A) in IP
Detection of human SF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SF1 antibody A303-214A used for IP at 6 µg/mg lysate. SF1 was also immunoprecipitated by rabbit anti-SF1 antibody A303-213A, which recognizes an upstream epitope. For blotting immunoprecipitated SF1, A303-213A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.
Product Details
A303-214A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 225 and 275 of Human Splicing Factor 1.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
tris citrate/phosphate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Wet ice
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Mouse
Target Information
Splicing factor 1 is a nuclear pre-mRNA splicing factor. The protein specifically recognizes the intron branch point sequence at the 3' splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The protein contains an N-terminal U2AF ligand motif, a central hnRNP K homology motif and quaking 2 region which bind a key branch-site adenosine within the branch point sequence, a zinc knuckles domain, and a C-terminal proline-rich domain. Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BBP; M ZFM1; Mammalian branch point-binding protein; Splicing factor 1; Transcription factor ZFM1; Zinc finger gene in MEN1 locus; Zinc finger protein 162
Gene Aliases: BBP; D11S636; MBBP; SF1; ZCCHC25; ZFM1; ZNF162
UniProt ID: (Human) Q15637
Entrez Gene ID: (Human) 7536
spliceosomal complex assembly
mRNA 3'-splice site recognition
mRNA splicing, via spliceosome
transcription, DNA-templated
regulation of transcription, DNA-templated
male sex determination
Leydig cell differentiation
negative regulation of smooth muscle cell proliferation
regulation of steroid biosynthetic process
negative regulation of nucleic acid-templated transcription
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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