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FIGURE: 1 / 1
SF3A1 Antibody (PA5-117000) in WB
Western blot analysis of SF3A1 in extracts of various cell lines. Samples were incubated with SF3A1 Polyclonal antibody (Product # PA5-117000) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Product Details
PA5-117000
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 715-785 of human SF3A1 (Q15459)
Conjugate
Form
Liquid
Concentration
2.21 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2901630
Product Specific Information
Positive Samples: Mouse lung, Rat brain, Rat lung
Immunogen sequence: QDKTEWKLNG QVLVFTLPLT DQVSVIKVKI HEATGMPAGK QKLQYEGIFI KDSNSLAYYN MANGAVIHLA L
Target Information
This gene encodes subunit 1 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. Subunit 1 belongs to the SURP protein family; named for the SURP motifs that are thought to mediate RNA binding. Subunit 1 has tandemly repeated SURP motifs in its amino-terminal half while its carboxy-terminal half contains a proline-rich region and a ubiquitin-like domain. Binding studies with truncated subunit 1 derivatives demonstrated that the two SURP motifs are necessary for binding to subunit 3 while contacts with subunit 2 may occur through sequences carboxy-terminal to the SURP motifs. Alternative splicing results in multiple transcript variants encoding different isoforms.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: SF3a120; splicing factor 3 subunit 1; Splicing factor 3A subunit 1
Gene Aliases: 1200014H24Rik; 5930416L09Rik; AI159724; Sf3a1
UniProt ID: (Mouse) Q8K4Z5
Entrez Gene ID: (Mouse) 67465, (Rat) 305479
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