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SF3A1 Antibody (MA5-26468) in IHC (P)
Immunohistochemistry was performed on paraffin-embedded adenocarcinoma of human endometrium tissue. To expose target proteins, 10mM citric buffer, pH6.0, 120°C for 3min was used. Following antigen retrieval, tissues were probed with a SF3A1 monoclonal antibody (Product # MA5-26468).
Product Details
MA5-26468
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI4D8
Immunogen
Human recombinant protein fragment corresponding to amino acids 249-568 of SF3A1 produced in E.coli
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 1% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2723644
Target Information
This gene encodes subunit 1 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. Subunit 1 belongs to the SURP protein family; named for the SURP motifs that are thought to mediate RNA binding. Subunit 1 has tandemly repeated SURP motifs in its amino-terminal half while its carboxy-terminal half contains a proline-rich region and a ubiquitin-like domain. Binding studies with truncated subunit 1 derivatives demonstrated that the two SURP motifs are necessary for binding to subunit 3 while contacts with subunit 2 may occur through sequences carboxy-terminal to the SURP motifs. Alternative splicing results in multiple transcript variants encoding different isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: pre-mRNA processing 21; pre-mRNA splicing factor SF3a (120 kDa subunit); SAP 114; SF3a120; Spliceosome-associated protein 114; splicing factor 3 subunit 1; Splicing factor 3A subunit 1; splicing factor 3a, subunit 1, 120kD; splicing factor 3a, subunit 1, 120kDa
Gene Aliases: PRP21; PRPF21; SAP114; SF3A1; SF3A120
UniProt ID: (Human) Q15459
Entrez Gene ID: (Human) 10291
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