Proteintech

SFI1 Polyclonal Antibody

SFI1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 5

SFI1 Antibody (13550-1-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells using 13550-1-AP (SFI1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

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Product Details

13550-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 4 publications 4 publications

Immunocytochemistry (ICC/IF)

1:10-1:100

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

SFI1 Fusion Protein Ag4268 (917-1211 aa encoded by BC033613)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.23 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MAQAHPSLL ADLGLAFPDF QSPYKIGDPT EISDPPAAPG QPRGTDPMFL SSNTAHSARK QPRRPHFLLE PAQSQRPQKP QEHGLGMAQP AAPSLTRPFL AEAPTALVPH SPLPGALSSA PGPKQPPTAS TGPELLLLPL SSFMPCGAAA PARVSAQRAT PRDKPPVPSS LASVPDPHLL LPGDFSATRA GPGLSTAGSL DLEAELEEIQ QQLLHYQTTK QNLWSCRRQA SSLRRWLELN REEPGPEDQE VEQQVQKELE QVEMQIQLLA EELQAQRQPI GACVARIQAL RQALC (917-1211 aa encoded by BC033613)

Target Information

SFI1 gene ontology annotations related to this gene include negative regulation of phosphatase activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Human SFI1 and Centrin form a complex critical for centriole architecture and ciliogenesis.

The EMBO journal

Laporte MH,Bouhlel IB,Bertiaux E,Morrison CG,Giroud A,Borgers S,Azimzadeh J,Bornens M,Guichard P,Paoletti A,Hamel V

Wed Nov 02 00:00:00 EDT 2022

SFI1 promotes centriole duplication by recruiting USP9X to stabilize the microcephaly protein STIL.

The Journal of cell biology

Kodani A,Moyer T,Chen A,Holland A,Walsh CA,Reiter JF

Mon Jul 01 00:00:00 EDT 2019

Differential requirements for the EF-hand domains of human centrin 2 in primary ciliogenesis and nucleotide excision repair.

Journal of cell science

Khouj EM,Prosser SL,Tada H,Chong WM,Liao JC,Sugasawa K,Morrison CG

Thu Oct 03 00:00:00 EDT 2019

Aurora B opposes PP1 function in mitosis by phosphorylating the conserved PP1-binding RVxF motif in PP1 regulatory proteins.

Science signaling

Nasa I,Rusin SF,Kettenbach AN,Moorhead GB

Tue May 15 00:00:00 EDT 2018

Aurora B opposes PP1 function in mitosis by phosphorylating the conserved PP1-binding RVxF motif in PP1 regulatory proteins.

Science signaling

Nasa I,Rusin SF,Kettenbach AN,Moorhead GB

Tue May 15 00:00:00 EDT 2018

Dietary glucoraphanin prevents the onset of psychosis in the adult offspring after maternal immune activation.

Scientific reports

Matsuura A,Ishima T,Fujita Y,Iwayama Y,Hasegawa S,Kawahara-Miki R,Maekawa M,Toyoshima M,Ushida Y,Suganuma H,Kida S,Yoshikawa T,Iyo M,Hashimoto K

Thu Feb 01 00:00:00 EST 2018

Dietary glucoraphanin prevents the onset of psychosis in the adult offspring after maternal immune activation.

Scientific reports

Matsuura A,Ishima T,Fujita Y,Iwayama Y,Hasegawa S,Kawahara-Miki R,Maekawa M,Toyoshima M,Ushida Y,Suganuma H,Kida S,Yoshikawa T,Iyo M,Hashimoto K

Thu Feb 01 00:00:00 EST 2018

Bioinformatics

Protein Aliases: homolog of yeast Sfi1; hSFI1; protein phosphatase 1, regulatory subunit 139; Protein SFI1 homolog; Sfi1 homolog, spindle assembly associated

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Gene Aliases: hSfi1p; KIAA0542; PISD; PPP1R139; SFI1

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UniProt ID: (Human) A8K8P3, (Mouse) Q3UZY0

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Entrez Gene ID: (Human) 9814, (Mouse) 78887

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Function(s)

protein binding phosphatase binding

Process(es)

G2/M transition of mitotic cell cycle negative regulation of phosphatase activity

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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SFI1 Polyclonal Antibody

SFI1 Antibody (13550-1-AP) in ICC/IF

Product Details

13550-1-AP

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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