Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
SFPQ Polyclonal Antibody
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 12
SFPQ Antibody (PA5-29948) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of SFPQ was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: SFPQ Polyclonal Antibody (Product # PA5-29948) diluted at 1:500. Red: phalloidin, a cytoskeleton marker. Scale bar = 10 µm.
Product Details
PA5-29948
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 230 and 449 of Human SFPQ
Conjugate
Form
Liquid
Concentration
0.78 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2547422
Product Specific Information
Recommended positive controls: 293T, A431, HeLa, HepG2, Neuro 2A, C8D30, NIH-3T3, Raw264.7, C2C12.
Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (85%), Chimpanzee (100%), Bovine (100%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
SFPQ, also named PSF, encodes a nuclear factor implicated in the splicing and regulation of gene expression. SFPQ probably forms a heteromer with NONO and participates in DNA pairing and DNA break repair program. Very recently SFPQ was identified as a downstream target of tau, complete nuclear depletion and cytoplasmic accumulation of SFPQ were shown in the neurons and astrocytes of brains with Alzheimer's disease (AD), more strikingly, reduced SFPQ levels may progress together with tau pathology, these observation strongly suggests the important role of SFPQ pathology in neurodegenerative diseases including AD. SFPQ protein may appears as 47 kDa, 52 kDa or 100 kDa molecules in various cell types.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: 100 kDa DNA-pairing protein; DNA-binding p52/p100 complex, 100 kDa subunit; hPOMp100; polypyrimidine tract binding protein associated; polypyrimidine tract-binding protein-associated splicing factor; Polypyrimidine tract-binding protein-associated-splicing factor; protein phosphatase 1, regulatory subunit 140; PSF; PTB-associated splicing factor; PTB-associated-splicing factor; splicing factor proline/glutamine rich (polypyrimidine tract binding protein associated); splicing factor proline/glutamine rich (polypyrimidine tract-binding protein-associated); splicing factor proline/glutamine-rich; Splicing factor, proline- and glutamine-rich
Gene Aliases: 1110004P21Rik; 2810416M14Rik; 5730453G22Rik; 9030402K04Rik; AU021830; D4Ertd314e; Gm12940; OTTMUSG00000009329; POMP100; PPP1R140; PSF; REP1; SFPQ
UniProt ID: (Human) P23246, (Mouse) Q8VIJ6
Entrez Gene ID: (Human) 6421, (Mouse) 71514
nucleotide binding
transcription regulatory region sequence-specific DNA binding
RNA polymerase II distal enhancer sequence-specific DNA binding
core promoter binding
chromatin binding
protein binding
histone deacetylase binding
transcription regulatory region DNA binding
poly(A) RNA binding
nucleic acid binding
DNA binding
RNA binding
RNA metabolism protein
negative regulation of transcription from RNA polymerase II promoter
alternative mRNA splicing, via spliceosome
double-strand break repair via homologous recombination
transcription, DNA-templated
mRNA processing
RNA splicing
regulation of circadian rhythm
negative regulation of circadian rhythm
positive regulation of sister chromatid cohesion
negative regulation of transcription, DNA-templated
rhythmic process
histone H3 deacetylation
positive regulation of oxidative stress-induced intrinsic apoptotic signaling pathway
DNA repair
regulation of transcription, DNA-templated
cellular response to DNA damage stimulus
chromosome organization
regulation of cell cycle
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support