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SFTPA2 Antibody (PA5-115987) in ICC/IF
Immunocytochemistry analysis of SFTPA2 in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115987) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
Product Details
PA5-115987
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human SFTPA2(Accession Q8IWL1), corresponding to amino acid residues G43-E93.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2900621
Product Specific Information
Antibody detects endogenous levels of total Surfactant Protein A.
Target Information
This gene is one of several genes encoding pulmonary-surfactant associated proteins (SFTPA) located on chromosome 10. Mutations in this gene and a highly similar gene located nearby, which affect the highly conserved carbohydrate recognition domain, are associated with idiopathic pulmonary fibrosis. The current version of the assembly displays only a single centromeric SFTPA gene pair rather than the two gene pairs shown in the previous assembly which were thought to have resulted from a duplication.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 35 kDa pulmonary surfactant-associated protein; Alveolar proteinosis protein; collectin 5; Collectin-5; PSP-A; Pulmonary surfactant-associated protein A2; SP-2A beta; SP-2A gamma; SP-A2; SP-A2 alpha; SP-A2 delta; SPA2; Surfactant Protein A2; surfactant, pulmonary-associated protein A2A
Gene Aliases: COLEC5; PSAP; PSP-A; PSPA; SFTP1; SFTPA; SFTPA2; SFTPA2B; SP-2A; SP-A; SPA2; SPAII
UniProt ID: (Human) Q8IWL1
Entrez Gene ID: (Human) 729238
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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