Invitrogen

SFTPC Polyclonal Antibody

4 Published Figures

9 References

View all (14) SFTPC antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (4)
Published Figures (4)

SFTPC Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 8

SFTPC Antibody (PA5-71680) in ICC/IF

Immunocytochemistry analysis of SFTPC in A549 cells. Samples were incubated with SFTPC polyclonal antibody (Product # PA5-71680) using a dilution of 1:25 followed by Dylight® 488-conjugated goat anti-Rabbit IgG at a dilution of 1:200 (green). Cells were 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized. Immunofluorescence image showing cytoplasm staining on A549 cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1:500 dilution (red). The nuclear counter stain is DAPI (blue).

SFTPC Antibody in Immunohistochemistry (Paraffin) (IHC (P))

SFTPC Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

SFTPC Antibody in Immunohistochemistry (IHC)

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Product Details

PA5-71680

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200

Immunocytochemistry (ICC/IF)

1:25

View 3 publications 3 publications

Flow Cytometry (Flow)

1:25

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human SFTPC

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A, Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Dry ice (international)

RRID

AB_2717534

Target Information

This gene encodes the pulmonary-associated surfactant protein C (SPC), an extremely hydrophobic surfactant protein essential for lung function and homeostasis after birth. Pulmonary surfactant is a surface-active lipoprotein complex composed of 90% lipids and 10% proteins which include plasma proteins and apolipoproteins SPA, SPB, SPC and SPD. The surfactant is secreted by the alveolar cells of the lung and maintains the stability of pulmonary tissue by reducing the surface tension of fluids that coat the lung. Multiple mutations in this gene have been identified, which cause pulmonary surfactant metabolism dysfunction type 2, also called pulmonary alveolar proteinosis due to surfactant protein C deficiency, and are associated with interstitial lung disease in older infants, children, and adults. Alternatively spliced transcript variants encoding different protein isoforms have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Activating NO-sGC crosstalk in the mouse vascular niche promotes vascular integrity and mitigates acute lung injury.

The Journal of experimental medicine

He H,Yang W,Su N,Zhang C,Dai J,Han F,Singhal M,Bai W,Zhu X,Zhu J,Liu Z,Xia W,Liu X,Zhang C,Jiang K,Huang W,Chen D,Wang Z,He X,Kirchhoff F,Li Z,Liu C,Huan J,Wang X,Wei W,Wang J,Augustin HG,Hu J

PA5-71680 was used in Immunohistochemistry to demonstrate that impaired NO-sGC crosstalk in the vascular niche results in elevated vascular permeability, and pharmacological activation of this crosstalk represents a promising translational therapy for ALI.

Mon Feb 06 00:00:00 EST 2023

Interstitial lung disease in children with Rubinstein-Taybi syndrome.

Pediatric pulmonology

Bradford L,Ross MK,Minso J,Cernelc-Kohan M,Shayan K,Wong SS,Li X,Rivier L,Jegga AG,Deutsch GH,Vece TJ,Loughlin CE,Gower WA,Hurley C,Furman W,Stokes D,Hagood JS

PA5-71680 was used in Immunocytochemistry, Western Blot, Immunohistochemistry to present three patients with RSTS who developed ILD and discuss possible mechanisms by which a mutation in CREBBP may be involved in the development of ILD.Routine hematoxylin and eosin staining was performed on lung biopsy tissue for histological analysis.

Sat Jan 01 00:00:00 EST 2022

Interstitial lung disease in children with Rubinstein-Taybi syndrome.

Pediatric pulmonology

Bradford L,Ross MK,Minso J,Cernelc-Kohan M,Shayan K,Wong SS,Li X,Rivier L,Jegga AG,Deutsch GH,Vece TJ,Loughlin CE,Gower WA,Hurley C,Furman W,Stokes D,Hagood JS

Sat Jan 01 00:00:00 EST 2022

Prkg2 regulates alveolar type 2-mediated re-alveolarization.

Stem cell research & therapy

Zhang M,Ali G,Komatsu S,Zhao R,Ji HL

PA5-71680 was used in Immunocytochemistry to demonstrate that Prkg2 may regulate the lineage of AT2 cells, which is afected by endotoxins and the interactive PKA signaling pathway.

Mon Mar 21 00:00:00 EDT 2022

Prkg2 regulates alveolar type 2-mediated re-alveolarization.

Stem cell research & therapy

Zhang M,Ali G,Komatsu S,Zhao R,Ji HL

PA5-71680 was used in Immunocytochemistry to demonstrate that Prkg2 may regulate the lineage of AT2 cells, which is afected by endotoxins and the interactive PKA signaling pathway.

Mon Mar 21 00:00:00 EDT 2022

YAP and Wnt3a independently promote AECIIs proliferation and differentiation by increasing nuclear β‑catenin expression in experimental bronchopulmonary dysplasia.

International journal of molecular medicine

Jia X,Wu B,Huang J,Fan L,Yang M,Xu W

PA5-71680 was used in Immunocytochemistry to demonstrate that YAP and Wnt3a independently promote AECII proliferation and differentiation in experimental BPD by increasing the nuclear β‚catenin levels.

Fri Jan 01 00:00:00 EST 2021

YAP and Wnt3a independently promote AECIIs proliferation and differentiation by increasing nuclear β‑catenin expression in experimental bronchopulmonary dysplasia.

International journal of molecular medicine

Jia X,Wu B,Huang J,Fan L,Yang M,Xu W

Fri Jan 01 00:00:00 EST 2021

YAP and Wnt3a independently promote AECIIs proliferation and differentiation by increasing nuclear β‑catenin expression in experimental bronchopulmonary dysplasia.

International journal of molecular medicine

Jia X,Wu B,Huang J,Fan L,Yang M,Xu W

Fri Jan 01 00:00:00 EST 2021

YAP and Wnt3a independently promote AECIIs proliferation and differentiation by increasing nuclear β‑catenin expression in experimental bronchopulmonary dysplasia.

International journal of molecular medicine

Jia X,Wu B,Huang J,Fan L,Yang M,Xu W

Fri Jan 01 00:00:00 EST 2021

Co-culture of type I and type II pneumocytes as a model of alveolar epithelium.

PloS one

Brookes O,Boland S,Lai Kuen R,Miremont D,Movassat J,Baeza-Squiban A

PA5-71680 was used in Immunocytochemistry to support the coculture of these two cell lines to produce a model which better represents the breadth of functions seen in native alveolar epithelium.

Fri Nov 12 00:00:00 EST 2021

Engineered amphiphilic peptides enable delivery of proteins and CRISPR-associated nucleases to airway epithelia.

Nature communications

Krishnamurthy S,Wohlford-Lenane C,Kandimalla S,Sartre G,Meyerholz DK,Théberge V,Hallée S,Duperré AM,Del'Guidice T,Lepetit-Stoffaes JP,Barbeau X,Guay D,McCray PB

PA5-71680 was used in Immunohistochemistry to achieve protein and SpCas9 or AsCas12a ribonucleoprotein (RNP) delivery to cultured human well-differentiated airway epithelial cells and mouse lungs with engineered amphiphilic peptides.

Mon Oct 28 00:00:00 EDT 2019

Bioinformatics

Protein Aliases: BRICHOS domain containing 6; PSP C; pulmonary surfactant apoprotein-2 SP-C; Pulmonary surfactant-associated protein C; Pulmonary surfactant-associated proteolipid SPL(Val); SFTP 2; SP C; SP-C; SP5; surfactant associated protein C; Surfactant protein C; Surfactant pulmonary-associated protein C; Surfactant, pulmonary-associated protein C

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Gene Aliases: BRICD6; pro-SpC; PSP-C; Sftp-2; SFTP2; SFTPC; SMDP2; SP-C; SP5; SPC

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UniProt ID: (Human) P11686, (Rat) P11685

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Entrez Gene ID: (Human) 6440, (Mouse) 20389, (Rat) 50683

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Function(s)

protein binding protein homodimerization activity surfactant structural protein

Process(es)

respiratory gaseous exchange cellular protein metabolic process protein homooligomerization circadian rhythm response to hormone response to glucose response to organic substance response to lipopolysaccharide response to retinoic acid response to vitamin A regulation of liquid surface tension response to glucocorticoid response to cAMP response to hyperoxia response to interleukin-6 response to growth factor cellular response to mechanical stimulus cellular response to nitric oxide

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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SFTPC Polyclonal Antibody

SFTPC Antibody (PA5-71680) in ICC/IF

Product Details

PA5-71680

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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