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FIGURE: 1 / 1
SGEF Antibody (PA5-109410) in WB
Western blot analysis of SGEF in extracts of various cell lines. Samples were incubated with SGEF Polyclonal antibody (Product # PA5-109410) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
PA5-109410
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 592-871 of human ARHGEF26 (NP_056410.3).
Conjugate
Form
Liquid
Concentration
1.75 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2854821
Product Specific Information
Immunogen sequence: ICQKTPKDSP KYEVCKRALK EVSKLVRLCN EGARKMERTE MMYTINSQLE FKIKPFPLVS SSRWLVKRGE LTAYVEDTVL FSRRTSKQQV YFFLFNDVLI ITKKKSEESY NVNDYSLRDQ LLVESCDNEE LNSSPGKNSS TMLYSRQSSA SHLFTLTVLS NHANEKVEML LGAETQSERA RWITALGHSS GKPPADRTSL TQVEIVRSFT AKQPDELSLQ VADVVLIYQR VSDGWYEGER LRDGERGWFP MECAKEITCQ ATIDKNVERM GRLLGLETNV
Target Information
Activates RhoG GTPase by promoting the exchange of GDP by GTP. Required for the formation of membrane ruffles during macropinocytosis. Required for the formation of cup-like structures during trans-endothelial migration of leukocytes. In case of Salmonella enterica infection, activated by SopB, which induces cytoskeleton rearrangements and promotes bacterial entry.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Rho guanine nucleotide exchange factor (GEF) 26; Rho guanine nucleotide exchange factor 26; SH3 domain-containing guanine exchange factor; Src homology 3 domain-containing guanine nucleotide exchange factor; testicular tissue protein Li 171
Gene Aliases: 4631416L12Rik; 8430436L14Rik; AI303572; ARHGEF26; AW491953; CSGEF; HMFN1864; RGD1562559; SGEF
UniProt ID: (Human) Q96DR7
Entrez Gene ID: (Human) 26084, (Mouse) 622434, (Rat) 310460
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