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SH3BGRL Antibody (PA5-104140) in ICC/IF
Immunocytochemistry analysis of SH3BGRL in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with SH3BGRL polyclonal antibody (Product # PA5-104140) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-104140
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human SH3BGRL(Accession O75368), corresponding to amino acid residues N56-A106.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2853468
Product Specific Information
Antibody detects endogenous levels of total SH3BGRL1.
Target Information
SH3BGRL belongs to the SH3BGR family. Mutations in predicted EVH1-binding domain of SH3BGRL had a modest effect on suppression of v-Rel transformation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Adapter SH3BGRL; epididymis secretory protein Li 115; SH3 domain binding glutamate-rich protein like; SH3 domain binding glutamic acid-rich protein like; SH3 domain-binding glutamic acid-rich-like protein; SH3 domain-binding glutamic acid-rich-like protein 1; SH3-binding domain glutamic acid-rich protein like
Gene Aliases: 1190008F14Rik; HEL-S-115; RGD1560520; SH3BGR; SH3BGRL
UniProt ID: (Human) O75368, (Mouse) Q9JJU8
Entrez Gene ID: (Human) 6451, (Mouse) 56726, (Rat) 302363
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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