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SHANK1 Antibody (MA5-45447) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% PFA for 15 min. Samples were incubated with SHANK1 monoclonal antibody (Product # MA5-45447) at 1:50 for overnight at 4°C with slow rocking, followed by AlexaFluor 488 at 1:1,000 for 1 hour at RT. Counterstain used was Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) SHANK1 antibody (D) Composite.
Product Details
MA5-45447
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
N22/21
Immunogen
Fusion protein amino acids 469-691 (SH3/PDZ domains) of rat Shank1
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
9.1mM sodium bicarbonate/PBS, pH 7.4, with 581mM DMSO, 123mM ethanolamine
Contains
no preservative
Storage conditions
4° C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2931901
Product Specific Information
1 µg/mL of MA5-45447 was sufficient for detection of Shank1 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 190-220 kDa (alternative splice variants). No cross-reactivity against Shank2 or Shank3.
Target Information
Seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors via complexes with GKAP/PSD-95 and Homer, respectively, and the actin-based cytoskeleton. May play a role in the structural and functional organization of the dendritic spine and synaptic junction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: GKAP/SAPAP interacting protein; GKAP/SAPAP-interacting protein; OTTHUMP00000174437; SAPAP-interacting protein synamon; SH3 and multiple ankyrin repeat domains 1; SH3 and multiple ankyrin repeat domains protein 1; SH3/ankyrin domain gene 1; SHANK 1; Shank1; Somatostatin receptor-interacting protein; SPANK 1; SPANK-1; SSTR-interacting protein; Synamon
Gene Aliases: SHANK1; Shank1a; SPANK-1; Spank1; SSTRIP; synamon
UniProt ID: (Human) Q9Y566, (Rat) Q9WV48, (Mouse) D3YZU1
Entrez Gene ID: (Human) 50944, (Rat) 78957, (Mouse) 243961
protein binding
protein C-terminus binding
SH3 domain binding
GKAP/Homer scaffold activity
somatostatin receptor binding
protein complex binding
ionotropic glutamate receptor binding
identical protein binding
ankyrin repeat binding
scaffold protein binding
receptor signaling complex scaffold activity
protein complex assembly
cytoskeletal anchoring at plasma membrane
long-term memory
associative learning
adult behavior
negative regulation of actin filament bundle assembly
social behavior
protein localization to synapse
olfactory behavior
habituation
neuromuscular process controlling balance
determination of affect
righting reflex
synapse maturation
dendritic spine morphogenesis
positive regulation of dendritic spine development
vocalization behavior
regulation of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity
positive regulation of excitatory postsynaptic potential
nervous system development
cell differentiation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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