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Invitrogen
SHARPIN Monoclonal Antibody (OTI1F4)
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 4
SHARPIN Antibody (MA5-27500) in WB
Western blot was performed using Anti-SHARPIN Monoclonal Antibody (OTI1F4) (Product # MA5-27500) and a 42 kDa band corresponding to SHARPIN was observed across the cell lines tested. An uncharacterized band of ~ 37 kDa was also observed in the cell lines. Whole cell extracts (30 µg lysate) of PC-3 (Lane1), DU 145 (Lane 2), MDA-MB-231 (Lane 3), MCF7 (Lane 4), HeLa (Lane 5), A549 (Lane 6) and Jurkat (Lane 7) were electrophoresed using Novex® NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto ... View More
Product Details
MA5-27500
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI1F4
Immunogen
Human recombinant protein fragment corresponding to amino acids 1-326 of SHARPIN produced in E.coli
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 1% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2725345
Target Information
Component of the LUBAC complex which conjugates linear polyubiquitin chains in a head-to-tail manner to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is proposed to be recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Together with FAM105B/otulin, the LUBAC complex regulates the canonical Wnt signaling during angiogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: hSIPL1; SHANK-associated RH domain interactor; Shank-associated RH domain-interacting protein; Shank-interacting protein-like 1; Sharpin
Gene Aliases: PSEC0216; SHARPIN; SIPL1
UniProt ID: (Human) Q9H0F6
Entrez Gene ID: (Human) 81858
mitochondrion organization
I-kappaB kinase/NF-kappaB signaling
brain development
regulation of tumor necrosis factor-mediated signaling pathway
apoptotic nuclear changes
keratinization
positive regulation of I-kappaB kinase/NF-kappaB signaling
proteasome-mediated ubiquitin-dependent protein catabolic process
negative regulation of inflammatory response
protein homooligomerization
protein linear polyubiquitination
regulation of CD40 signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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