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SHFM3 Antibody (PA5-96951) in WB
Western blot analysis of SHFM3 in mouse brain. Samples were incubated with SHFM3 Polyclonal antibody (Product # PA5-96951) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-96951
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 143-412 of human FBXW4 (NP_0713221)
Conjugate
Form
Liquid
Concentration
0.56 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2808753
Product Specific Information
Immunogen sequence: QFRPDGASLN RRPLGVFAGH DEDVCHFVLA NSHIVSAGGD GKIGIHKIHS TFTVKYSAHE QEVNCVDCKG GIIVSGSRDR TAKVWPLASG RLGQCLHTIQ TEDRVWSIAI SPLLSSFVTG TACCGHFSPL RIWDLNSGQL MTHLGSDFPP GAGVLDVMYE SPFTLLSCGY DTYVRYWDLR TSVRKCVMEW EEPHDSTLYC LQTDGNHLLA TGSSYYGVVR LWDRRQRACL HAFPLTSTPL SSPVYCLRLT TKHLYAALSY NLHVLDFQNP; Positive Samples: Mouse brain
Target Information
This gene is a member of the F-box/WD-40 gene family, which recruit specific target proteins through their WD-40 protein-protein binding domains for ubiquitin mediated degradation. In mouse, a highly similar protein is thought to be responsible for maintaining the apical ectodermal ridge of developing limb buds; disruption of the mouse gene results in the absence of central digits, underdeveloped or absent metacarpal/metatarsal bones and syndactyly. This phenotype is remarkably similar to split hand-split foot malformation in humans, a clinically heterogeneous condition with a variety of modes of transmission. An autosomal recessive form has been mapped to the chromosomal region where this gene is located, and complex rearrangements involving duplications of this gene and others have been associated with the condition. A pseudogene of this locus has been mapped to one of the introns of the BCR gene on chromosome 22.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: dactylaplasia; F-box and WD-40 domain-containing protein 4; F-box protein Fbw4; F-box/WD repeat-containing protein 4; Protein hagoromo
Gene Aliases: Dac; dactylin; dactylyn; Fbw4; Fbxw4; SHFM3; SHSF3
UniProt ID: (Mouse) Q9JMJ2
Entrez Gene ID: (Mouse) 30838
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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