Invitrogen

SHH Recombinant Rabbit Monoclonal Antibody (23H15L1)

View all (27) SHH antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

701403

Applications
Tested Dilution
Publications

Western Blot (WB)

0.5-3 µg/mL
-

Immunocytochemistry (ICC/IF)

2 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

23H15L1

Immunogen

Peptide corresponding to amino acids 173-184 of human SHH.
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2532481

Product Specific Information

This antibody is predicted to react with canine, non-human primate, mouse, rabbit, rat and porcine based on sequence homology.

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

Sonic Hedgehog (SHH), which is expressed only during embryogenesis, is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the Sonic Hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved, the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE), a disorder in which the developing forebrain fails to correctly separate into right and left hemispheres. HPE is manifested by facial deformities. In addition, it is thought that mutations in this gene or in its signalling pathway may be responsible for VACTERL syndrome, which is characterized by vertebral defects, anal atresia, tracheoesophageal fistula with esophageal atresia, radial and renal dysplasia, cardiac anomalies, and limb abnormalities.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: hemimelic extra toes; HHG-1; SHH; Shh unprocessed N-terminal signaling and C-terminal autoprocessing domains; ShhNC; short digits; sonic hedgehog homolog; Sonic hedgehog protein

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Gene Aliases: 9530036O11Rik; Dsh; HHG1; HLP3; HPE3; Hx; Hxl3; M100081; MCOPCB5; SHH; SMMCI; TPT; TPTPS

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UniProt ID: (Human) Q15465, (Mouse) Q62226

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Entrez Gene ID: (Human) 6469, (Mouse) 20423

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Function(s)
glycoprotein binding patched binding calcium ion binding protein binding glycosaminoglycan binding peptidase activity zinc ion binding morphogen activity laminin-1 binding hydrolase activity metal ion binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter angiogenesis patterning of blood vessels vasculogenesis metanephros development branching involved in ureteric bud morphogenesis cell fate specification neural crest cell migration kidney development neural tube formation hair follicle development vasculature development heart looping positive regulation of neuroblast proliferation positive regulation of mesenchymal cell proliferation osteoblast development lymphoid progenitor cell differentiation determination of left/right asymmetry in lateral mesoderm regulation of transcription, DNA-templated proteolysis endocytosis apoptotic process cell communication signal transduction smoothened signaling pathway positive regulation of hh target transcription factor activity cell-cell signaling multicellular organism development determination of left/right symmetry pattern specification process ectoderm development neuroblast proliferation axon guidance central nervous system development hindgut morphogenesis digestive tract mesoderm development heart development blood coagulation androgen metabolic process cell proliferation positive regulation of cell proliferation embryo development polarity specification of anterior/posterior axis anterior/posterior pattern specification dorsal/ventral pattern formation mesenchymal cell proliferation regulation of gene expression positive regulation of gene expression negative regulation of gene expression oligodendrocyte development striated muscle tissue development positive regulation of skeletal muscle cell proliferation myotube differentiation intein-mediated protein splicing spinal cord dorsal/ventral patterning ventral spinal cord interneuron specification spinal cord motor neuron differentiation thalamus development dorsal/ventral neural tube patterning cerebellar granule cell precursor proliferation smoothened signaling pathway involved in regulation of cerebellar granule cell precursor cell proliferation positive regulation of cerebellar granule cell precursor proliferation telencephalon regionalization establishment of cell polarity regulation of proteolysis positive regulation of Wnt signaling pathway negative regulation of Wnt signaling pathway respiratory tube development lung development embryonic limb morphogenesis negative regulation of cell migration male genitalia development prostate gland development thyroid gland development forebrain development midbrain development hindbrain development pancreas development hair follicle morphogenesis negative regulation of proteasomal ubiquitin-dependent protein catabolic process T cell differentiation in thymus positive regulation of T cell differentiation in thymus positive regulation of immature T cell proliferation in thymus negative regulation of transcription elongation from RNA polymerase II promoter protein localization to nucleus embryonic forelimb morphogenesis embryonic hindlimb morphogenesis regulation of cell proliferation negative regulation of T cell proliferation negative regulation of protein catabolic process positive regulation of protein import into nucleus odontogenesis of dentin-containing tooth odontogenesis regulation of odontogenesis embryonic digit morphogenesis camera-type eye development negative regulation of apoptotic process CD4-positive or CD8-positive, alpha-beta T cell lineage commitment tongue development tongue morphogenesis skin development positive thymic T cell selection negative thymic T cell selection intermediate filament organization cell fate commitment myoblast differentiation response to ethanol negative regulation of cell differentiation positive regulation of cell differentiation positive regulation of neuron differentiation positive regulation of smoothened signaling pathway positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of photoreceptor cell differentiation positive regulation of alpha-beta T cell differentiation negative regulation of alpha-beta T cell differentiation cell development thymus development digestive tract morphogenesis embryonic digestive tract morphogenesis embryonic organ development developmental growth embryonic morphogenesis embryonic foregut morphogenesis positive regulation of skeletal muscle tissue development organ formation anatomical structure formation involved in morphogenesis neuron fate commitment response to axon injury embryonic skeletal system development oligodendrocyte differentiation positive regulation of oligodendrocyte differentiation branching morphogenesis of an epithelial tube male genitalia morphogenesis inner ear development anatomical structure development formation of anatomical boundary stem cell development striated muscle cell differentiation positive regulation of striated muscle cell differentiation positive regulation of cell division Bergmann glial cell differentiation palate development canonical Wnt signaling pathway limb development limb bud formation positive regulation of penile erection lung morphogenesis lung epithelium development trachea development trachea morphogenesis epithelial tube branching involved in lung morphogenesis branching involved in prostate gland morphogenesis branching involved in salivary gland morphogenesis bud outgrowth involved in lung branching right lung development left lung development lung lobe morphogenesis lung-associated mesenchyme development prostatic bud formation primary prostatic bud elongation prostate epithelial cord elongation salivary gland cavitation epithelial cell proliferation involved in salivary gland morphogenesis epithelial-mesenchymal cell signaling regulation of prostatic bud formation epithelial-mesenchymal signaling involved in prostate gland development regulation of epithelial cell proliferation involved in prostate gland development positive regulation of epithelial cell proliferation involved in prostate gland development regulation of mesenchymal cell proliferation involved in prostate gland development mesenchymal smoothened signaling pathway involved in prostate gland development artery development mesenchymal cell proliferation involved in lung development vasculogenesis involved in coronary vascular morphogenesis somite development positive regulation of sclerotome development fungiform papilla development fungiform papilla morphogenesis fungiform papilla formation positive regulation of oligodendrocyte progenitor proliferation cellular response to lithium ion metanephric mesenchymal cell proliferation involved in metanephros development multicellular structure septum development negative regulation of canonical Wnt signaling pathway negative regulation of cholesterol efflux apoptotic signaling pathway regulation of protein localization to nucleus negative regulation of ureter smooth muscle cell differentiation positive regulation of ureter smooth muscle cell differentiation negative regulation of kidney smooth muscle cell differentiation positive regulation of kidney smooth muscle cell differentiation positive regulation of mesenchymal cell proliferation involved in ureter development negative regulation of mesenchymal cell apoptotic process ventral midline development embryonic pattern specification dopaminergic neuron differentiation renal system development regulation of nodal signaling pathway involved in determination of lateral mesoderm left/right asymmetry negative regulation of dopaminergic neuron differentiation
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