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SHMT1 Antibody (PA5-100183) in IHC (P)
Immunohistochemistry analysis of SHMT1 in human colorectal cancer. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with SHMT1 polyclonal antibody (Product # PA5-100183) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
Product Details
PA5-100183
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human SHMT1(Accession P34896), corresponding to amino acid residues V376-H426.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2815713
Product Specific Information
Antibody detects endogenous levels of total SHMT1.
Target Information
This gene encodes the cellular form of serine hydroxymethyltransferase, a pyridoxal phosphate-containing enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. This reaction provides one carbon units for synthesis of methionine, thymidylate, and purines in the cytoplasm. This gene is located within the Smith-Magenis syndrome region on chromosome 17. Alternative splicing of this gene results in 2 transcript variants encoding 2 different isoforms. Additional transcript variants have been described, but their biological validity has not been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cytoplasmic serine hydroxymethyltransferase; Glycine hydroxymethyltransferase; MGC15229; MGC24556; serine hydroxymethyltransferase 1 (soluble); Serine hydroxymethyltransferase, cytosolic; Serine methylase; SHMT
Gene Aliases: AI324848; AI385541; C81125; CSHMT; LRRGT00032; mShmt; mshmt1; mshmt2; SHMT; SHMT1
UniProt ID: (Human) P34896, (Mouse) P50431
Entrez Gene ID: (Human) 6470, (Mouse) 20425, (Rat) 287379
glycine hydroxymethyltransferase activity
protein binding
L-allo-threonine aldolase activity
amino acid binding
pyridoxal phosphate binding
protein homodimerization activity
serine binding
catalytic activity
transferase activity
identical protein binding
methyltransferase
transferase
metabolite interconversion enzyme
dTMP biosynthetic process
glycine metabolic process
L-serine metabolic process
L-serine catabolic process
purine nucleobase biosynthetic process
glycine biosynthetic process from serine
tetrahydrofolate interconversion
carnitine biosynthetic process
tetrahydrofolate metabolic process
folic acid metabolic process
protein tetramerization
protein homotetramerization
cellular response to tetrahydrofolate
glycine biosynthetic process
one-carbon metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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