Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 4
SHMT1 Antibody (MA5-49246) in ICC/IF
Immunocytochemistry analysis of SHMT1 in A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in SHMT1 Monoclonal antibody (Product # MA5-49246) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for... View More
Product Details
MA5-49246
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
9C7
Immunogen
E.coli-derived human SHMT1 recombinant protein (Position: M1-S470).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_3074904
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Target Information
This gene encodes the cellular form of serine hydroxymethyltransferase, a pyridoxal phosphate-containing enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. This reaction provides one carbon units for synthesis of methionine, thymidylate, and purines in the cytoplasm. This gene is located within the Smith-Magenis syndrome region on chromosome 17. Alternative splicing of this gene results in 2 transcript variants encoding 2 different isoforms. Additional transcript variants have been described, but their biological validity has not been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: cytoplasmic serine hydroxymethyltransferase; Glycine hydroxymethyltransferase; MGC15229; MGC24556; serine hydroxymethyltransferase 1 (soluble); Serine hydroxymethyltransferase, cytosolic; Serine methylase; SHMT
Gene Aliases: AI324848; AI385541; C81125; CSHMT; LRRGT00032; mShmt; mshmt1; mshmt2; SHMT; SHMT1
UniProt ID: (Human) P34896, (Mouse) P50431
Entrez Gene ID: (Human) 6470, (Mouse) 20425, (Rat) 287379
glycine hydroxymethyltransferase activity
protein binding
L-allo-threonine aldolase activity
amino acid binding
pyridoxal phosphate binding
protein homodimerization activity
serine binding
catalytic activity
transferase activity
identical protein binding
methyltransferase
transferase
metabolite interconversion enzyme
dTMP biosynthetic process
glycine metabolic process
L-serine metabolic process
L-serine catabolic process
purine nucleobase biosynthetic process
glycine biosynthetic process from serine
tetrahydrofolate interconversion
carnitine biosynthetic process
tetrahydrofolate metabolic process
folic acid metabolic process
protein tetramerization
protein homotetramerization
cellular response to tetrahydrofolate
glycine biosynthetic process
one-carbon metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support