Proteintech

SHMT2 Polyclonal Antibody

SHMT2 Antibody in Immunocytochemistry (ICC/IF)

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SHMT2 Antibody (11099-1-AP) in ICC/IF

Immunofluorescent analysis of MCF-7 cells using 11099-1-AP (SHMT2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

SHMT2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

11099-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 16 publications 16 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:20-1:200

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

SHMT2 Fusion Protein Ag1584 (205-504 aa encoded by BC011911)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: DYNQLALTA RLFRPRLIIA GTSAYARLID YARMREVCDE VKAHLLADMA HISGLVAAKV IPSPFKHADI VTTTTHKTLR GARSGLIFYR KGVKAVDPKT GREIPYTFED RINFAVFPSL QGGPHNHAIA AVAVALKQAC TPMFREYSLQ VLKNARAMAD ALLERGYSLV SGGTDNHLVL VDLRPKGLDG ARAERVLELV SITANKNTCP GDRSAITPGG LRLGAPALTS RQFREDDFRR VVDFIDEGVN IGLEVKSKTA KLQDFKSFLL KDSETSQRLA NLRQRVEQFA RAFPMPGFDE H (205-504 aa encoded by BC011911)

Target Information

Mammalian serine hydroxymethyltransferase (SHMT) is a tetrameric, pyridoxal phosphate (PLP)-dependent enzyme that catalyzes the reversible interconversion of serine and tetrahydrofolate to glycine and methylenetetrahydrofolate in the cytoplasm (cSHMT, SHMT1) and mitochondria (mSHMT, SHMT2). cSHMT preferentially supplies one-carbon units for thymidylate biosynthesis, depletes methylenetetrahydrofolate pools for S-adenosylmethionine (SAM) synthesis by synthesizing serine, sequesters 5-methyltetrahydrofolate, and inhibits SAM synthesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Serine hydroxymethyltransferase 2 knockdown induces apoptosis in ccRCC by causing lysosomal membrane permeabilization via metabolic reprogramming.

Cell death & disease

Liu Z,Fan M,Hou J,Pan S,Xu Y,Zhang H,Liu C,Hao X,Li X,Wang H

Mon Feb 20 00:00:00 EST 2023

SHMT2 promotes the tumorigenesis of renal cell carcinoma by regulating the m6A modification of PPAT.

Genomics

Huo FC,Xie M,Zhu ZM,Zheng JN,Pei DS

Fri Jul 01 00:00:00 EDT 2022

SHMT2 promotes the tumorigenesis of renal cell carcinoma by regulating the m6A modification of PPAT.

Genomics

Huo FC,Xie M,Zhu ZM,Zheng JN,Pei DS

Fri Jul 01 00:00:00 EDT 2022

Transcriptome and Proteome Analysis in LUHMES Cells Overexpressing Alpha-Synuclein.

Frontiers in neurology

Höllerhage M,Stepath M,Kohl M,Pfeiffer K,Chua OWH,Duan L,Hopfner F,Eisenacher M,Marcus K,Höglinger GU

Sat Apr 30 00:00:00 EDT 2022

Overexpression of SHMT2 Predicts a Poor Prognosis and Promotes Tumor Cell Growth in Bladder Cancer.

Frontiers in genetics

Zhang P,Yang Q

Tue Jun 22 00:00:00 EDT 2021

Derangement of hepatic polyamine, folate, and methionine cycle metabolism in cystathionine beta-synthase-deficient homocystinuria in the presence and absence of treatment: Possible implications for pathogenesis.

Molecular genetics and metabolism

Maclean KN,Jiang H,Phinney WN,Mclagan BM,Roede JR,Stabler SP

Mon Feb 01 00:00:00 EST 2021

Generation of SHMT2 knockout human embryonic stem cell line (WAe009-A-67) using CRISPR/Cas9 technique.

Stem cell research

Li J,Zhang B,Gan M,Li Y,He L,Yue W,Qiao H,Pei X,Li Y

Wed Dec 01 00:00:00 EST 2021

Generation of SHMT2 knockout human embryonic stem cell line (WAe009-A-67) using CRISPR/Cas9 technique.

Stem cell research

Li J,Zhang B,Gan M,Li Y,He L,Yue W,Qiao H,Pei X,Li Y

Wed Dec 01 00:00:00 EST 2021

Melatonin modulates metabolic remodeling in HNSCC by suppressing MTHFD1L-formate axis.

Journal of pineal research

Cui L,Zhao X,Jin Z,Wang H,Yang SF,Hu S

Wed Dec 01 00:00:00 EST 2021

TGF-β Promotes Metabolic Reprogramming in Lung Fibroblasts via mTORC1-dependent ATF4 Activation.

American journal of respiratory cell and molecular biology

O'Leary EM,Tian Y,Nigdelioglu R,Witt LJ,Cetin-Atalay R,Meliton AY,Woods PS,Kimmig LM,Sun KA,Gökalp GA,Mutlu GM,Hamanaka RB

Sun Nov 01 00:00:00 EDT 2020

Defective NADPH production in mitochondrial disease complex I causes inflammation and cell death.

Nature communications

Balsa E,Perry EA,Bennett CF,Jedrychowski M,Gygi SP,Doench JG,Puigserver P

Mon Jun 01 00:00:00 EDT 2020

De novo synthesis of serine and glycine fuels purine nucleotide biosynthesis in human lung cancer tissues.

The Journal of biological chemistry

Fan TWM,Bruntz RC,Yang Y,Song H,Chernyavskaya Y,Deng P,Zhang Y,Shah PP,Beverly LJ,Qi Z,Mahan AL,Higashi RM,Dang CV,Lane AN

Fri Sep 06 00:00:00 EDT 2019

Inhibition of Phosphoglycerate Dehydrogenase Attenuates Bleomycin-induced Pulmonary Fibrosis.

American journal of respiratory cell and molecular biology

Hamanaka RB,Nigdelioglu R,Meliton AY,Tian Y,Witt LJ,O'Leary E,Sun KA,Woods PS,Wu D,Ansbro B,Ard S,Rohde JM,Dulin NO,Guzy RD,Mutlu GM

Tue May 01 00:00:00 EDT 2018

Inhibition of Phosphoglycerate Dehydrogenase Attenuates Bleomycin-induced Pulmonary Fibrosis.

American journal of respiratory cell and molecular biology

Hamanaka RB,Nigdelioglu R,Meliton AY,Tian Y,Witt LJ,O'Leary E,Sun KA,Woods PS,Wu D,Ansbro B,Ard S,Rohde JM,Dulin NO,Guzy RD,Mutlu GM

Tue May 01 00:00:00 EDT 2018

Inhibition of Phosphoglycerate Dehydrogenase Attenuates Bleomycin-induced Pulmonary Fibrosis.

American journal of respiratory cell and molecular biology

Hamanaka RB,Nigdelioglu R,Meliton AY,Tian Y,Witt LJ,O'Leary E,Sun KA,Woods PS,Wu D,Ansbro B,Ard S,Rohde JM,Dulin NO,Guzy RD,Mutlu GM

Tue May 01 00:00:00 EDT 2018

Inhibition of Phosphoglycerate Dehydrogenase Attenuates Bleomycin-induced Pulmonary Fibrosis.

American journal of respiratory cell and molecular biology

Hamanaka RB,Nigdelioglu R,Meliton AY,Tian Y,Witt LJ,O'Leary E,Sun KA,Woods PS,Wu D,Ansbro B,Ard S,Rohde JM,Dulin NO,Guzy RD,Mutlu GM

Tue May 01 00:00:00 EDT 2018

SHMT2 and the BRCC36/BRISC deubiquitinase regulate HIV-1 Tat K63-ubiquitylation and destruction by autophagy.

PLoS pathogens

Xu M,Moresco JJ,Chang M,Mukim A,Smith D,Diedrich JK,Yates JR,Jones KA

Tue May 01 00:00:00 EDT 2018

SHMT2 and the BRCC36/BRISC deubiquitinase regulate HIV-1 Tat K63-ubiquitylation and destruction by autophagy.

PLoS pathogens

Xu M,Moresco JJ,Chang M,Mukim A,Smith D,Diedrich JK,Yates JR,Jones KA

Tue May 01 00:00:00 EDT 2018

Vascular endothelial growth factor signaling requires glycine to promote angiogenesis.

Scientific reports

Guo D,Murdoch CE,Xu H,Shi H,Duan DD,Ahmed A,Gu Y

Tue Nov 07 00:00:00 EST 2017

Vascular endothelial growth factor signaling requires glycine to promote angiogenesis.

Scientific reports

Guo D,Murdoch CE,Xu H,Shi H,Duan DD,Ahmed A,Gu Y

Tue Nov 07 00:00:00 EST 2017

'Rod and ring' formation from IMP dehydrogenase is regulated through the one-carbon metabolic pathway.

Journal of cell science

Calise SJ,Purich DL,Nguyen T,Saleem DA,Krueger C,Yin JD,Chan EK

Mon Aug 01 00:00:00 EDT 2016

'Rod and ring' formation from IMP dehydrogenase is regulated through the one-carbon metabolic pathway.

Journal of cell science

Calise SJ,Purich DL,Nguyen T,Saleem DA,Krueger C,Yin JD,Chan EK

Mon Aug 01 00:00:00 EDT 2016

'Rod and ring' formation from IMP dehydrogenase is regulated through the one-carbon metabolic pathway.

Journal of cell science

Calise SJ,Purich DL,Nguyen T,Saleem DA,Krueger C,Yin JD,Chan EK

Mon Aug 01 00:00:00 EDT 2016

'Rod and ring' formation from IMP dehydrogenase is regulated through the one-carbon metabolic pathway.

Journal of cell science

Calise SJ,Purich DL,Nguyen T,Saleem DA,Krueger C,Yin JD,Chan EK

Mon Aug 01 00:00:00 EDT 2016

Transforming Growth Factor (TGF)-β Promotes de Novo Serine Synthesis for Collagen Production.

The Journal of biological chemistry

Nigdelioglu R,Hamanaka RB,Meliton AY,O'Leary E,Witt LJ,Cho T,Sun K,Bonham C,Wu D,Woods PS,Husain AN,Wolfgeher D,Dulin NO,Chandel NS,Mutlu GM

Fri Dec 30 00:00:00 EST 2016

Transforming Growth Factor (TGF)-β Promotes de Novo Serine Synthesis for Collagen Production.

The Journal of biological chemistry

Nigdelioglu R,Hamanaka RB,Meliton AY,O'Leary E,Witt LJ,Cho T,Sun K,Bonham C,Wu D,Woods PS,Husain AN,Wolfgeher D,Dulin NO,Chandel NS,Mutlu GM

Fri Dec 30 00:00:00 EST 2016

Transforming Growth Factor (TGF)-β Promotes de Novo Serine Synthesis for Collagen Production.

The Journal of biological chemistry

Nigdelioglu R,Hamanaka RB,Meliton AY,O'Leary E,Witt LJ,Cho T,Sun K,Bonham C,Wu D,Woods PS,Husain AN,Wolfgeher D,Dulin NO,Chandel NS,Mutlu GM

Fri Dec 30 00:00:00 EST 2016

Transforming Growth Factor (TGF)-β Promotes de Novo Serine Synthesis for Collagen Production.

The Journal of biological chemistry

Nigdelioglu R,Hamanaka RB,Meliton AY,O'Leary E,Witt LJ,Cho T,Sun K,Bonham C,Wu D,Woods PS,Husain AN,Wolfgeher D,Dulin NO,Chandel NS,Mutlu GM

Fri Dec 30 00:00:00 EST 2016

cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions.

Cell research

Sun L,Song L,Wan Q,Wu G,Li X,Wang Y,Wang J,Liu Z,Zhong X,He X,Shen S,Pan X,Li A,Wang Y,Gao P,Tang H,Zhang H

Wed Apr 01 00:00:00 EDT 2015

cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions.

Cell research

Sun L,Song L,Wan Q,Wu G,Li X,Wang Y,Wang J,Liu Z,Zhong X,He X,Shen S,Pan X,Li A,Wang Y,Gao P,Tang H,Zhang H

Wed Apr 01 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: epididymis secretory sperm binding protein Li 51e; GLY A+; glycine auxotroph A, human complement for hamster; Glycine hydroxymethyltransferase; serine aldolase; serine hydroxymethylase; serine hydroxymethyltransferase 2 (mitochondrial); Serine hydroxymethyltransferase, mitochondrial; Serine methylase; SHMT; SHMT 2; threonine aldolase

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Gene Aliases: 2700043D08Rik; AA408223; AA986903; GLYA; HEL-S-51e; SHMT; SHMT2

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UniProt ID: (Human) P34897, (Mouse) Q9CZN7

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Entrez Gene ID: (Human) 6472, (Mouse) 108037, (Rat) 299857

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Function(s)

chromatin binding glycine hydroxymethyltransferase activity protein binding L-allo-threonine aldolase activity amino acid binding pyridoxal phosphate binding identical protein binding methyltransferase activity methyltransferase

Process(es)

glycine metabolic process L-serine metabolic process L-serine biosynthetic process one-carbon metabolic process positive regulation of cell proliferation glycine biosynthetic process from serine response to type I interferon tetrahydrofolate interconversion tetrahydrofolate metabolic process folic acid metabolic process protein tetramerization protein homotetramerization protein K63-linked deubiquitination glycine biosynthetic process methylation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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SHMT2 Polyclonal Antibody

SHMT2 Antibody (11099-1-AP) in ICC/IF

Product Details

11099-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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