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Invitrogen
SHMT2 Polyclonal Antibody
Advanced Verification
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 13
SHMT2 Antibody (PA5-32228) in ICC/IF
Immunofluorescence analysis of Serine hydroxymethyltransferase, mitochondrial was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with SHMT2 Polyclonal Antibody (Product # PA5-32228) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Seco... View More
Product Details
PA5-32228
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein fragment corresponding to a region within amino acids 1 and 483 of Human SHMT2
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2549701
Product Specific Information
Recommended positive controls: 293T, HeLa, A431, HepG2.
Predicted reactivity: Mouse (93%), Rat (91%), Xenopus laevis (81%), Rabbit (93%), Bovine (92%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Mammalian serine hydroxymethyltransferase (SHMT) is a tetrameric, pyridoxal phosphate (PLP)-dependent enzyme that catalyzes the reversible interconversion of serine and tetrahydrofolate to glycine and methylenetetrahydrofolate in the cytoplasm (cSHMT, SHMT1) and mitochondria (mSHMT, SHMT2). cSHMT preferentially supplies one-carbon units for thymidylate biosynthesis, depletes methylenetetrahydrofolate pools for S-adenosylmethionine (SAM) synthesis by synthesizing serine, sequesters 5-methyltetrahydrofolate, and inhibits SAM synthesis.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: epididymis secretory sperm binding protein Li 51e; GLY A+; glycine auxotroph A, human complement for hamster; Glycine hydroxymethyltransferase; serine aldolase; serine hydroxymethylase; serine hydroxymethyltransferase 2 (mitochondrial); Serine hydroxymethyltransferase, mitochondrial; Serine methylase; SHMT; SHMT 2; threonine aldolase
Gene Aliases: 2700043D08Rik; AA408223; AA986903; GLYA; HEL-S-51e; SHMT; SHMT2
UniProt ID: (Human) P34897, (Mouse) Q9CZN7
Entrez Gene ID: (Human) 6472, (Mouse) 108037, (Rat) 299857
glycine metabolic process
L-serine metabolic process
L-serine biosynthetic process
one-carbon metabolic process
positive regulation of cell proliferation
glycine biosynthetic process from serine
response to type I interferon
tetrahydrofolate interconversion
tetrahydrofolate metabolic process
folic acid metabolic process
protein tetramerization
protein homotetramerization
protein K63-linked deubiquitination
glycine biosynthetic process
methylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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