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SHP-1 Antibody (PA5-115896) in IHC (P)
Immunohistochemistry analysis of SHP-1 in rat lung tissue. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-115896) at a dilution of 1:100. Secondary staining was applied with HRP conjugated goat anti-rabbit.
Product Details
PA5-115896
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human PTPN6(Accession P29350), corresponding to amino acid residues V510-K560.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2900530
Product Specific Information
Antibody detects endogenous levels of total SHP1.
Target Information
SHP-1 (PTPN6) is a non-receptor protein tyrosine phosphatase that is expressed primarily in hematopoietic cells. The enzyme is composed of two SH2 domains, a tyrosine phosphatase catalytic domain, and a carboxy-terminal regulatory domain. SHP-1 removes phosphates from target proteins to downregulate several tyrosine kinase-regulated pathways. In hematopoietic cells, the amino-terminal SH2 domain of SHP-1 binds to tyrosine phosphorylated erythropoietin receptors (EpoR) to negatively regulate hematopoietic growth. Overexpression of SHP-1 in epithelial cells results in dephosphorylation of the Ros receptor tyrosine kinase and subsequent downregulation of Ros-dependent cell proliferation and transformation. Following ligand binding in myeloid cells, SHP-1 associates with the IL-3R beta chain and downregulates IL-3-induced tyrosine phosphorylation and cell proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 70Z-SHP; EC 3.1.3.48; hematopoietic cell phosphatase; Hematopoietic cell protein-tyrosine phosphatase; motheaten; Protein-tyrosine phosphatase 1C; Protein-tyrosine phosphatase SHP-1; PTN6; PTP-1C; PTPTY-42; SH-PTP1; SH2 phosphatase 1; SHP-1; Tyrosine-protein phosphatase non-receptor type 6
Gene Aliases: 70Z-SHP; HCP; HCPH; HPTP1C; me; PTP-1C; PTP1C; Ptph6; PTPN6; PTPTY-42; SH-PTP1; SHP-1; SHP-1L; SHP1
UniProt ID: (Human) P29350, (Rat) P81718, (Mouse) P29351
Entrez Gene ID: (Human) 5777, (Rat) 116689, (Mouse) 15170
phosphotyrosine binding
protein tyrosine phosphatase activity
transmembrane receptor protein tyrosine phosphatase activity
protein binding
SH3 domain binding
protein kinase binding
SH2 domain binding
natural killer cell lectin-like receptor binding
phosphoprotein phosphatase activity
hydrolase activity
phosphatase activity
protein phosphatase
hematopoietic progenitor cell differentiation
negative regulation of humoral immune response mediated by circulating immunoglobulin
protein dephosphorylation
apoptotic process
G-protein coupled receptor signaling pathway
cell proliferation
positive regulation of cell proliferation
negative regulation of cell proliferation
positive regulation of phosphatidylinositol 3-kinase signaling
peptidyl-tyrosine phosphorylation
cell differentiation
platelet activation
platelet formation
T cell costimulation
abortive mitotic cell cycle
positive regulation of cell adhesion mediated by integrin
peptidyl-tyrosine dephosphorylation
intracellular signal transduction
megakaryocyte development
negative regulation of T cell proliferation
natural killer cell mediated cytotoxicity
negative regulation of MAP kinase activity
regulation of B cell differentiation
negative regulation of peptidyl-tyrosine phosphorylation
B cell receptor signaling pathway
negative regulation of B cell receptor signaling pathway
negative regulation of T cell receptor signaling pathway
leukocyte migration
regulation of release of sequestered calcium ion into cytosol
regulation of type I interferon-mediated signaling pathway
regulation of ERK1 and ERK2 cascade
platelet aggregation
regulation of G1/S transition of mitotic cell cycle
response to wounding
negative regulation of MAPK cascade
signal transduction
dephosphorylation
cytokine-mediated signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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