Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Product Details
PA5-86238
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide, corresponding to amino acids 500-550 of Human SHP-1.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2803028
Product Specific Information
Purity is > 95% by SDS-PAGE.
Target Information
SHP-1 (PTPN6) is a non-receptor protein tyrosine phosphatase that is expressed primarily in hematopoietic cells. The enzyme is composed of two SH2 domains, a tyrosine phosphatase catalytic domain, and a carboxy-terminal regulatory domain. SHP-1 removes phosphates from target proteins to downregulate several tyrosine kinase-regulated pathways. In hematopoietic cells, the amino-terminal SH2 domain of SHP-1 binds to tyrosine phosphorylated erythropoietin receptors (EpoR) to negatively regulate hematopoietic growth. Overexpression of SHP-1 in epithelial cells results in dephosphorylation of the Ros receptor tyrosine kinase and subsequent downregulation of Ros-dependent cell proliferation and transformation. Following ligand binding in myeloid cells, SHP-1 associates with the IL-3R beta chain and downregulates IL-3-induced tyrosine phosphorylation and cell proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: 70Z-SHP; EC 3.1.3.48; hematopoietic cell phosphatase; Hematopoietic cell protein-tyrosine phosphatase; motheaten; Protein-tyrosine phosphatase 1C; Protein-tyrosine phosphatase SHP-1; PTN6; PTP-1C; PTPTY-42; SH-PTP1; SH2 phosphatase 1; SHP-1; Tyrosine-protein phosphatase non-receptor type 6
Gene Aliases: 70Z-SHP; HCP; HCPH; HPTP1C; me; PTP-1C; PTP1C; Ptph6; PTPN6; PTPTY-42; SH-PTP1; SHP-1; SHP-1L; SHP1
UniProt ID: (Human) P29350, (Rat) P81718, (Mouse) P29351
Entrez Gene ID: (Human) 5777, (Rat) 116689, (Mouse) 15170
phosphotyrosine binding
protein tyrosine phosphatase activity
transmembrane receptor protein tyrosine phosphatase activity
protein binding
SH3 domain binding
protein kinase binding
SH2 domain binding
natural killer cell lectin-like receptor binding
phosphoprotein phosphatase activity
hydrolase activity
phosphatase activity
protein phosphatase
hematopoietic progenitor cell differentiation
negative regulation of humoral immune response mediated by circulating immunoglobulin
protein dephosphorylation
apoptotic process
G-protein coupled receptor signaling pathway
cell proliferation
positive regulation of cell proliferation
negative regulation of cell proliferation
positive regulation of phosphatidylinositol 3-kinase signaling
peptidyl-tyrosine phosphorylation
cell differentiation
platelet activation
platelet formation
T cell costimulation
abortive mitotic cell cycle
positive regulation of cell adhesion mediated by integrin
peptidyl-tyrosine dephosphorylation
intracellular signal transduction
megakaryocyte development
negative regulation of T cell proliferation
natural killer cell mediated cytotoxicity
negative regulation of MAP kinase activity
regulation of B cell differentiation
negative regulation of peptidyl-tyrosine phosphorylation
B cell receptor signaling pathway
negative regulation of B cell receptor signaling pathway
negative regulation of T cell receptor signaling pathway
leukocyte migration
regulation of release of sequestered calcium ion into cytosol
regulation of type I interferon-mediated signaling pathway
regulation of ERK1 and ERK2 cascade
platelet aggregation
regulation of G1/S transition of mitotic cell cycle
response to wounding
negative regulation of MAPK cascade
signal transduction
dephosphorylation
cytokine-mediated signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support