Invitrogen

SIAH1/SIAH2 Polyclonal Antibody

View all (3) SIAH1/SIAH2 antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

PA5-36274

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:1,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:50-1:200
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to amino acids 178-229 of human Siah-1
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

15mM sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2553449

Product Specific Information

This antibody detects endogenous protein at a molecular weight of 40 kDa.

Purity is >95% by SDS-PAGE.

Target Information

SIAH, the human homologue of the Drosophila seven in absentia (sina) gene, is a tumor suppressor protein that is expressed in intestinal epithelium and activated during apoptosis. Human SIAH proteins are produced as two distinct gene products, SIAH-1, and a slightly larger protein SIAH-2, which share a highly conserved C-terminal sequence and differ in their N-terminal regions. SIAH-1 is a 32 kDa protein that contains an N-terminal RING-finger domain, which is required for proteolysis, and a cystein-rich C-terminal domain, which regulates oligomerization and SIAH binding to target proteins. As a tumor suppressor, SIAH-1 binds DCC (deleted in colorectal cancer) and regulates DCC degradation via the ubiquitin-proteasome pathway. SIAH-1 also binds a Bcl-2 related protein, Bag-1, thereby inhibiting cell growth. The majority of SIAH-1 is localized to the nucleus, however a small percentage is detected in the cytoplasm. This nuclear localization suggests that SIAH proteins may interact with other nuclear matrix proteins and DNA.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?
Let us know so we can reference it here.
Cite this product

Bioinformatics

Protein Aliases: E3 ubiquitin-protein ligase SIAH1; E3 ubiquitin-protein ligase SIAH2; EC 6.3.2; FLJ08065; hSIAH1; mSiah2; RING-type E3 ubiquitin transferase SIAH1; RING-type E3 ubiquitin transferase SIAH2; seven in absentia 1A; seven in absentia 2; Seven in absentia homolog 1; Seven in absentia homolog 2; Siah-1; Siah-1a; Siah-2

View more View less

Gene Aliases: AA415433; hSiah2; HUMSIAH; SIAH; SIAH1; SIAH1A; SIAH2; Sinh2

View more View less

UniProt ID: (Human) Q8IUQ4, (Human) O43255, (Rat) Q920M9, (Mouse) Q06986, (Rat) Q8R4T2

View more View less

Entrez Gene ID: (Human) 6477, (Human) 6478, (Rat) 140941, (Mouse) 20439, (Rat) 140593

View more View less

Function(s)
ubiquitin-protein transferase activity protein binding protein C-terminus binding zinc ion binding ligase activity identical protein binding ubiquitin protein ligase activity transcription corepressor activity ubiquitin conjugating enzyme binding protein homodimerization activity UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity ribosomal S6-glutamic acid ligase activity coenzyme F420-0 gamma-glutamyl ligase activity coenzyme F420-2 alpha-glutamyl ligase activity metal ion binding protein-glycine ligase activity protein-glycine ligase activity, initiating protein-glycine ligase activity, elongating tubulin-glycine ligase activity protein-glutamic acid ligase activity tubulin-glutamic acid ligase activity ubiquitin-protein ligase protein modifying enzyme
Process(es)
protein polyubiquitination ubiquitin-dependent protein catabolic process apoptotic process cell cycle spermatogenesis nervous system development axon guidance anatomical structure morphogenesis protein catabolic process protein destabilization protein ubiquitination involved in ubiquitin-dependent protein catabolic process positive regulation of apoptotic process proteasome-mediated ubiquitin-dependent protein catabolic process cellular protein metabolic process neuron apoptotic process positive regulation of intrinsic apoptotic signaling pathway small GTPase mediated signal transduction regulation of protein ubiquitination negative regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process cellular protein catabolic process negative regulation of canonical Wnt signaling pathway negative regulation of nucleic acid-templated transcription negative regulation of extrinsic apoptotic signaling pathway male meiosis I multicellular organism development post-embryonic development cell differentiation regulation of multicellular organism growth protein ubiquitination
guarantee_icon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more
help_icon

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support