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SIAH2 Polyclonal Antibody

SIAH2 Antibody in Immunocytochemistry (ICC/IF)

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SIAH2 Antibody (12651-1-AP) in ICC/IF

Immunofluorescent analysis of ( -20°C Ethanol) fixed MCF-7 cells using 12651-1-AP (SIAH2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

SIAH2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

SIAH2 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

12651-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200-1:1,000

View 8 publications 8 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:50-1:500

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

View 2 publications 2 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Not Applicable

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

SIAH2 Fusion Protein Ag3342 (1-324 aa encoded by BC013082)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.27 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MSRPSSTGP SANKPCSKQP PPQPQHTPSP AAPPAAATIS AAGPGSSAVP AAAAVISGPG GGGGAGPVSP QHHELTSLFE CPVCFDYVLP PILQCQAGHL VCNQCRQKLS CCPTCRGALT PSIRNLAMEK VASAVLFPCK YATTGCSLTL HHTEKPEHED ICEYRPYSCP CPGASCKWQG SLEAVMSHLM HAHKSITTLQ GEDIVFLATD INLPGAVDWV MMQSCFGHHF MLVLEKQEKY EGHQQFFAIV LLIGTRKQAE NFAYRLELNG NRRRLTWEAT PRSIHDGVAA AIMNSDCLVF DTAIAHLFAD NGNLGINVTI STCCP (1-324 aa encoded by BC013082)

Target Information

SIAH-2 (seven in absentia homolog 2) is an E3 ligase that catalyzes ubiquitination and proteasome-mediated degradation of protein substrates. SIAH-2 encodes a 324 amino acid protein that shares 77% identity with human SIAH-1 and 68% identity with the Drosophila sina (seven in absentia) gene, on which development of the Drosophila R7 photoreceptor is dependent. SIAH-2 targets TRAF2 (which regulates cell responses to stress and cytokines through the regulation of key stress-signaling cascades) for degradation under stress conditions such as hypoxia. It targets HIF-1 alpha prolyl hydroxylase 3 (PHD3) for degradation upon exposure to hypoxic conditions, which coincides with an increase in SIAH-2 transcription. SIAH-2 can decrease TNF-alpha-dependent induction of JNK activity and transcriptional activation of NFkB. SIAH-2 null mice subjected to hypoxia display an impaired respiratory response and reduced levels of hemoglobin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Methylene blue targets PHD3 expression in murine microglia to mitigate lipopolysaccharide-induced neuroinflammation and neurocognitive impairments.

International immunopharmacology

Ou G,Che J,Dong J,Deng Y,Jiang X,Sun Y,He Z,Chen W,Zhang J

Sat Jul 01 00:00:00 EDT 2023

Methylene blue targets PHD3 expression in murine microglia to mitigate lipopolysaccharide-induced neuroinflammation and neurocognitive impairments.

International immunopharmacology

Ou G,Che J,Dong J,Deng Y,Jiang X,Sun Y,He Z,Chen W,Zhang J

Sat Jul 01 00:00:00 EDT 2023

Hypoxia-induced proteasomal degradation of DBC1 by SIAH2 in breast cancer progression.

eLife

Liu Q,Luo Q,Feng J,Zhao Y,Ma B,Cheng H,Zhao T,Lei H,Mu C,Chen L,Meng Y,Zhang J,Long Y,Su J,Chen G,Li Y,Hu G,Liao X,Chen Q,Zhu Y

Mon Aug 01 00:00:00 EDT 2022

Hypoxia-induced proteasomal degradation of DBC1 by SIAH2 in breast cancer progression.

eLife

Liu Q,Luo Q,Feng J,Zhao Y,Ma B,Cheng H,Zhao T,Lei H,Mu C,Chen L,Meng Y,Zhang J,Long Y,Su J,Chen G,Li Y,Hu G,Liao X,Chen Q,Zhu Y

Mon Aug 01 00:00:00 EDT 2022

SIAH ubiquitin E3 ligases as modulators of inflammatory gene expression.

Heliyon

Schmitz ML,Dreute J,Pfisterer M,Günther S,Kracht M,Chillappagari S

Tue Mar 01 00:00:00 EST 2022

P-TEFb is degraded by Siah1/2 in quiescent cells.

Nucleic acids research

Huang F,Feng Y,Peterlin BM,Fujinaga K

Fri May 20 00:00:00 EDT 2022

Ochratoxin A Induces Steatosis via PPARγ-CD36 Axis.

Toxins

Zheng QW,Ding XF,Cao HJ,Ni QZ,Zhu B,Ma N,Zhang FK,Wang YK,Xu S,Chen TW,Xia J,Qiu XS,Yu DZ,Xie D,Li JJ

Sat Nov 13 00:00:00 EST 2021

Ochratoxin A Induces Steatosis via PPARγ-CD36 Axis.

Toxins

Zheng QW,Ding XF,Cao HJ,Ni QZ,Zhu B,Ma N,Zhang FK,Wang YK,Xu S,Chen TW,Xia J,Qiu XS,Yu DZ,Xie D,Li JJ

Sat Nov 13 00:00:00 EST 2021

Inhibition of Siah2 ubiquitin ligase ameliorates monocrotaline-induced pulmonary arterial remodeling through inactivation of YAP.

Life sciences

Wang Q,Shi W,Zhang Q,Feng W,Wang J,Zhai C,Yan X,Li M

Sat Feb 01 00:00:00 EST 2020

Heat stress activates YAP/TAZ to induce the heat shock transcriptome.

Nature cell biology

Luo M,Meng Z,Moroishi T,Lin KC,Shen G,Mo F,Shao B,Wei X,Zhang P,Wei Y,Guan KL

Tue Dec 01 00:00:00 EST 2020

The SIAH2-NRF1 axis spatially regulates tumor microenvironment remodeling for tumor progression.

Nature communications

Ma B,Cheng H,Mu C,Geng G,Zhao T,Luo Q,Ma K,Chang R,Liu Q,Gao R,Nie J,Xie J,Han J,Chen L,Ma G,Zhu Y,Chen Q

Mon Mar 04 00:00:00 EST 2019

The SIAH2-NRF1 axis spatially regulates tumor microenvironment remodeling for tumor progression.

Nature communications

Ma B,Cheng H,Mu C,Geng G,Zhao T,Luo Q,Ma K,Chang R,Liu Q,Gao R,Nie J,Xie J,Han J,Chen L,Ma G,Zhu Y,Chen Q

Mon Mar 04 00:00:00 EST 2019

PIWIL2 suppresses Siah2-mediated degradation of HDAC3 and facilitates CK2α-mediated HDAC3 phosphorylation.

Cell death & disease

Zhang Y,Zheng X,Tan H,Lu Y,Tao D,Liu Y,Ma Y

Sun Apr 01 00:00:00 EDT 2018

PIWIL2 suppresses Siah2-mediated degradation of HDAC3 and facilitates CK2α-mediated HDAC3 phosphorylation.

Cell death & disease

Zhang Y,Zheng X,Tan H,Lu Y,Tao D,Liu Y,Ma Y

Sun Apr 01 00:00:00 EDT 2018

Bioinformatics

Protein Aliases: E3 ubiquitin-protein ligase SIAH2; EC 6.3.2; mSiah2; RING-type E3 ubiquitin transferase SIAH2; seven in absentia 2; Seven in absentia homolog 2; Siah-2

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Gene Aliases: AA415433; hSiah2; SIAH2; Sinh2

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UniProt ID: (Human) O43255, (Mouse) Q06986, (Rat) Q8R4T2

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Entrez Gene ID: (Human) 6478, (Mouse) 20439, (Rat) 140593

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Function(s)

transcription corepressor activity ubiquitin-protein transferase activity protein binding zinc ion binding ligase activity ubiquitin conjugating enzyme binding ubiquitin protein ligase activity UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity ribosomal S6-glutamic acid ligase activity coenzyme F420-0 gamma-glutamyl ligase activity coenzyme F420-2 alpha-glutamyl ligase activity metal ion binding protein-glycine ligase activity protein-glycine ligase activity, initiating protein-glycine ligase activity, elongating tubulin-glycine ligase activity protein-glutamic acid ligase activity tubulin-glutamic acid ligase activity ubiquitin-protein ligase protein modifying enzyme

Process(es)

protein polyubiquitination ubiquitin-dependent protein catabolic process apoptotic process cell cycle small GTPase mediated signal transduction axon guidance regulation of protein ubiquitination protein ubiquitination involved in ubiquitin-dependent protein catabolic process negative regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process proteasome-mediated ubiquitin-dependent protein catabolic process cellular protein catabolic process cellular protein metabolic process negative regulation of canonical Wnt signaling pathway negative regulation of nucleic acid-templated transcription negative regulation of extrinsic apoptotic signaling pathway multicellular organism development protein ubiquitination

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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SIAH2 Polyclonal Antibody

SIAH2 Antibody (12651-1-AP) in ICC/IF

Product Details

12651-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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