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Product Details
MA5-24780
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Fruit fly,
Human,
Pig
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
24E6H3
Immunogen
Synthetic peptide made to an N-terminal portion of the drosophila SIAH2 protein (within residues 1-100).
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
tissue culture supernatant
Contains
0.1% sodium azide
Storage conditions
4° C, do not freeze
Shipping conditions
Wet ice
RRID
AB_2717253
Product Specific Information
This is specific for human SIAH2 and Drosophila SINA in proliferating cells and stem cells. This does not detect Drosophila SINAH or normal, nondividing cells.
Target Information
SIAH-2 (seven in absentia homolog 2) is an E3 ligase that catalyzes ubiquitination and proteasome-mediated degradation of protein substrates. SIAH-2 encodes a 324 amino acid protein that shares 77% identity with human SIAH-1 and 68% identity with the Drosophila sina (seven in absentia) gene, on which development of the Drosophila R7 photoreceptor is dependent. SIAH-2 targets TRAF2 (which regulates cell responses to stress and cytokines through the regulation of key stress-signaling cascades) for degradation under stress conditions such as hypoxia. It targets HIF-1 alpha prolyl hydroxylase 3 (PHD3) for degradation upon exposure to hypoxic conditions, which coincides with an increase in SIAH-2 transcription. SIAH-2 can decrease TNF-alpha-dependent induction of JNK activity and transcriptional activation of NFkB. SIAH-2 null mice subjected to hypoxia display an impaired respiratory response and reduced levels of hemoglobin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: anon-fast-evolving-2H7; CG9949-PA; CG9949-PB; CG9949-PC; E3 ubiquitin-protein ligase SIAH2; E3 ubiquitin-protein ligase sina; EC 6.3.2; HL08111p; RING-type E3 ubiquitin transferase SIAH2; RING-type E3 ubiquitin transferase sina; seven in absenti; Seven in absentia homolog 2; Seven in absentia protein; seven-in-absentia; Siah-2; sina-PA; sina-PB; sina-PC
Gene Aliases: anon-EST:fe2H7; CG9949; Dmel\CG9949; Dmel_CG9949; hSiah2; sia; SIAH2; SINA
UniProt ID: (Human) O43255, (Fruit fly) P21461
Entrez Gene ID: (Human) 6478, (Pig) 100622531, (Fruit fly) 39884
protein polyubiquitination
ubiquitin-dependent protein catabolic process
apoptotic process
cell cycle
small GTPase mediated signal transduction
axon guidance
regulation of protein ubiquitination
protein ubiquitination involved in ubiquitin-dependent protein catabolic process
negative regulation of apoptotic process
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
proteasome-mediated ubiquitin-dependent protein catabolic process
cellular protein catabolic process
cellular protein metabolic process
negative regulation of canonical Wnt signaling pathway
negative regulation of nucleic acid-templated transcription
negative regulation of extrinsic apoptotic signaling pathway
sensory organ development
R7 cell fate commitment
proteasomal protein catabolic process
protein ubiquitination
positive regulation of proteasomal ubiquitin-dependent protein catabolic process
enteroendocrine cell differentiation
sevenless signaling pathway
regulation of R7 cell differentiation
Performance Guarantee
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