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FIGURE: 1 / 1
SIM2 Antibody (PA5-90219) in WB
Western blot analysis of SIM2 in various lysates. Samples were incubated with SIM2 Polyclonal antibody (Product # PA5-90219) using a dilution of 1:400, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Product Details
PA5-90219
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 342-525 of human SIM2 (NP_0050601?)
Conjugate
Form
Liquid
Concentration
1.22 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2805967
Product Specific Information
Immunogen sequence: LSLEQVSTAK SQDSWRTALS TSQETRKLVK PKNTKMKTKL RTNPYPPQQY SSFQMDKLEC GQLGNWRASP PASAAAPPEL QPHSESSDLL YTPSYSLPFS YHYGHFPLDS HVFSSKKPML PAKFGQPQGS PCEVARFFLS TLPASGECQW HYANPLVPSS SSPAKNPPEP PANTARHSLV PSYE; Positive Samples: U-251MG, HepG2; Cellular Location: Nucleus
Target Information
This gene represents a homolog of the Drosophila single-minded (sim) gene, which encodes a transcription factor that is a master regulator of neurogenesis. The encoded protein is ubiquitinated by RING-IBR-RING-type E3 ubiquitin ligases, including the parkin RBR E3 ubiquitin protein ligase. This gene maps within the so-called Down syndrome chromosomal region, and is thus thought to contribute to some specific Down syndrome phenotypes. Alternative splicing of this gene results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: bHLHe15; Class E basic helix-loop-helix protein 15; mSIM; SIM transcription factor; single-minded 2; Single-minded homolog 2; transcription factor SIM2
Gene Aliases: BHLHE15; HMC13F06; HMC29C01; SIM; SIM2
UniProt ID: (Human) Q14190, (Mouse) Q61079
Entrez Gene ID: (Human) 6493, (Rat) 304071, (Mouse) 20465
RNA polymerase II transcription factor activity, sequence-specific DNA binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein heterodimerization activity
signal transducer activity
protein binding
protein dimerization activity
basic helix-loop-helix transcription factor
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
nervous system development
embryonic pattern specification
cell differentiation
lung development
signal transduction
negative regulation of transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
multicellular organism development
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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