Invitrogen

SIN3A Polyclonal Antibody

2 Published Figures

7 References

View all (21) SIN3A antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (6)
Published Figures (2)

SIN3A Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 8

SIN3A Antibody (PA1-870) in ICC/IF

Immunofluorescent analysis of Sin3A (green) showing staining in the nucleus of Hela cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Sin3A polyclonal antibody (Product # PA1-870) in 3% BSA-PBS at a dilution of 1:200 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated ... View More

SIN3A Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

Product Details

PA1-870

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5 µg/mL

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:50-1:500

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

ChIP assay (ChIP)

1-3 µL

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues M(1) K R R L D D Q E S P V Y A A Q Q R R(19) of mouse mSin3A.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2187625

Product Specific Information

PA1-870 detects mSin3A from human and mouse tissues and cells.

PA1-870 has been successfully used in Western blot, ICC/IF ChIP, IHC-P and immunoprecipitation procedures. By Western blot, this antibody detects an ~150 kDa protein representing mSin3A from T47D cell extract.

PA1-870 immunogen is a synthetic peptide corresponding to residues M(1) K R R L D D Q E S P V Y A A Q Q R R(19) of mouse mSin3A.

Target Information

Mammalian Sin3 (mSin3) is a 150 kDa protein closely related to the yeast SIN3 repressor protein involved in the transcriptional repression of many genes. There are two mammalian isoforms, mSin3A and mSin3B. Containing 4 paired amphipathic helix domains (PAH domains), mSin3A and mSin3B have been shown to directly interact with several other transcriptional repressor proteins including HDAC1, HDAC2, RbAp46, the methyl CpG binding protein MeCP2, the Mad/Max heterodimer, and the corepressors silencing mediator of retinoic acid and thyroid hormone receptor (SMRT) and nuclear receptor corepressor (N-CoR).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

An Epigenetic Mechanism Underlying Chromosome 17p Deletion-Driven Tumorigenesis.

Cancer discovery

Chen M,Chen X,Li S,Pan X,Gong Y,Zheng J,Xu J,Zhao C,Zhang Q,Zhang S,Qi L,Wang Z,Shi K,Ding BS,Xue Z,Chen L,Yang S,Wang Y,Niu T,Dai L,Lowe SW,Chen C,Liu Y

PA1-870 was used in Western Blot to identify a new 17p tumor suppressor gene (TSG), plant homeodomain finger protein 23 (PHF23).

Fri Jan 01 00:00:00 EST 2021

An Epigenetic Mechanism Underlying Chromosome 17p Deletion-Driven Tumorigenesis.

Cancer discovery

Chen M,Chen X,Li S,Pan X,Gong Y,Zheng J,Xu J,Zhao C,Zhang Q,Zhang S,Qi L,Wang Z,Shi K,Ding BS,Xue Z,Chen L,Yang S,Wang Y,Niu T,Dai L,Lowe SW,Chen C,Liu Y

PA1-870 was used in Western Blot to identify a new 17p tumor suppressor gene (TSG), plant homeodomain finger protein 23 (PHF23).

Fri Jan 01 00:00:00 EST 2021

Biotin tagging of MeCP2 in mice reveals contextual insights into the Rett syndrome transcriptome.

Nature medicine

Johnson BS,Zhao YT,Fasolino M,Lamonica JM,Kim YJ,Georgakilas G,Wood KH,Bu D,Cui Y,Goffin D,Vahedi G,Kim TH,Zhou Z

PA1-870 was used in Western Blotting to develop a Cre-inducible method for cell type-specific biotin tagging of MeCP2 in mice for studying the Rett syndrome transcriptome.

Sun Oct 01 00:00:00 EDT 2017

Elevating expression of MeCP2 T158M rescues DNA binding and Rett syndrome-like phenotypes.

The Journal of clinical investigation

Lamonica JM,Kwon DY,Goffin D,Fenik P,Johnson BS,Cui Y,Guo H,Veasey S,Zhou Z

Published figure using SIN3A polyclonal antibody (Product # PA1-870) in Western Blot

Mon May 01 00:00:00 EDT 2017

RNA-dependent dynamic histone acetylation regulates MCL1 alternative splicing.

Nucleic acids research

Khan DH,Gonzalez C,Cooper C,Sun JM,Chen HY,Healy S,Xu W,Smith KT,Workman JL,Leygue E,Davie JR

PA1-870 was used in western blot to study the modulation of MCL1 alternative splicing by an RNA-dependent mechanism involving dynamic histone acetylation

Sat Feb 01 00:00:00 EST 2014

Dynamic distribution of HDAC1 and HDAC2 during mitosis: association with F-actin.

Journal of cellular physiology

He S,Khan DH,Winter S,Seiser C,Davie JR

PA1-870 was used in immunocytochemistry to study the mitotic dynamics of HDAC-1 and -2 and their association with F-actin

Mon Jul 01 00:00:00 EDT 2013

Protein kinase CK2 regulates the dimerization of histone deacetylase 1 (HDAC1) and HDAC2 during mitosis.

The Journal of biological chemistry

Khan DH,He S,Yu J,Winter S,Cao W,Seiser C,Davie JR

PA1-870 was used in western blot to study the modulation of the dimerization of HDAC-1 and -2 by CK2-mediated phosphorylation during mitosis

Fri Jun 07 00:00:00 EDT 2013

Differential requirement of histone acetylase and deacetylase activities for IRF5-mediated proinflammatory cytokine expression.

Journal of immunology (Baltimore, Md. : 1950)

Feng D,Sangster-Guity N,Stone R,Korczeniewska J,Mancl ME,Fitzgerald-Bocarsly P,Barnes BJ

PA1-870 was used in immunoprecipitation and western blot to investigate the regulation of IRF5-mediated proinflammatory cytokine expression

Mon Nov 15 00:00:00 EST 2010

Bioinformatics

Protein Aliases: Histone deacetylase complex subunit Sin3a; Mammalian Sin3A; Paired amphipathic helix protein Sin3a; SIN; SIN3 homolog A, transcription regulator; SIN3 transcription regulator homolog A; SIN3A; transcriptional co-repressor Sin3A; Transcriptional corepressor Sin3a; transcriptional regulator, SIN3 yeast homolog A; transcriptional regulator, SIN3A

View more View less

Gene Aliases: AW553200; Kiaa4126; mKIAA4126; mSin3A; Sin3; SIN3A

View more View less

UniProt ID: (Human) Q96ST3, (Mouse) Q60520

View more View less

Entrez Gene ID: (Human) 25942, (Mouse) 20466

View more View less

Function(s)

transcription regulatory region sequence-specific DNA binding RNA polymerase II activating transcription factor binding RNA polymerase II repressing transcription factor binding RNA polymerase II transcription corepressor activity chromatin binding transcription factor activity, sequence-specific DNA binding RNA binding histone deacetylase activity protein binding protein complex binding protein deacetylase activity DNA binding transcription corepressor activity transcription factor binding chromatin/chromatin-binding, or -regulatory protein

Process(es)

negative regulation of transcription from RNA polymerase II promoter in utero embryonic development activation of innate immune response positive regulation of defense response to virus by host hematopoietic progenitor cell differentiation DNA replication transcription, DNA-templated protein deacetylation aging regulation of hormone levels positive regulation of G2/M transition of mitotic cell cycle histone deacetylation positive regulation of chromatin silencing cellular protein localization negative regulation of circadian rhythm negative regulation of apoptotic process regulation of transcription from RNA polymerase II promoter in response to oxidative stress cellular lipid metabolic process negative regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter rhythmic process response to methylglyoxal cellular response to glucose stimulus negative regulation of protein localization to nucleus negative regulation of histone H3-K27 acetylation cellular response to dopamine negative regulation of transcription regulatory region DNA binding regulation of transcription, DNA-templated transcription from RNA polymerase II promoter response to organonitrogen compound

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

SIN3A Polyclonal Antibody

SIN3A Antibody (PA1-870) in ICC/IF

Product Details

PA1-870

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!