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FIGURE: 1 / 3
SIN3B Antibody (PA5-30707) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of SIN3B was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: SIN3B Polyclonal Antibody (Product # PA5-30707) diluted at 1:500. Red: phalloidin, a cytoskeleton marker. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Product Details
PA5-30707
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 15 and 245 of Human SIN3B
Conjugate
Form
Liquid
Concentration
0.57 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2548181
Product Specific Information
Recommended positive controls: HepG2, HCT116, mouse brain.
Predicted reactivity: Mouse (92%), Rat (91%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Acts as a transcriptional repressor. Interacts with MXI1 to repress MYC responsive genes and antagonize MYC oncogenic activities. Interacts with MAD-MAX heterodimers by binding to MAD. The heterodimer then represses transcription by tethering SIN3B to DNA. Also forms a complex with FOXK1 which represses transcription.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Histone deacetylase complex subunit Sin3b; Paired amphipathic helix protein Sin3b; SIN3 homolog B, transcription regulator; SIN3 homolog B, transcriptional regulator; SIN3 transcription regulator homolog B; SIN3B; Transcriptional corepressor Sin3b; transcriptional regulator, SIN3 yeast homolog B
Gene Aliases: 2810430C10Rik; KIAA0700; SIN3B
UniProt ID: (Human) O75182, (Mouse) Q62141
Entrez Gene ID: (Human) 23309, (Mouse) 20467
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
skeletal muscle tissue development
histone deacetylation
cellular lipid metabolic process
cardiac muscle tissue development
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
negative regulation of cell cycle
negative regulation of transcription, DNA-templated
Performance Guarantee
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