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SIRP gamma Antibody (PA5-47627) in WB
Western blot analysis of SIRP gamma in NK‚92 human natural killer lymphoma cell line, Jurkat human acute T cell leukemia cell line, and human thymus tissue. Samples were incubated in SIRP gamma polyclonal antibody (Product # PA5-47627) using a dilution of 0.5 µg/mL followed by a HRP-conjugated Anti-Sheep IgG secondary antibody. Specific bands were detected for SIRPγ/CD172g at approximately 45-50 kDa (as indicated). This experiment was conducted under reducing conditions.
Product Details
PA5-47627
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Sheep
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
CHO-derived recombinant human SIRP gamma/CD172g Val64-Ser364, (Val263Ala) and (Ser286Leu)
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2576834
Product Specific Information
In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) SIRP alpha, rhSIRP beta 1, and recombinant mouse SIRP alpha is observed.
Reconstitute in sterile PBS to a final concentration of 0.2 mg/mL.
Target Information
SIRP gamma (CD172g) recognizes a 55 kDa signal regulatory protein which is a member of the immunoglobulin gene superfamily. SIRP gamma is expressed on most T lymphocytes and a subset of B-cells but is absent on myeloid cells. CD172g has a truncated cytoplasmic tail, which is similar to SIRP beta, but unlike SIRP beta CD172g does not require DAP12 for expression at the cell surface. CD172g, like SIRP alpha, binds to CD47 inducing apoptosis. SIRP gamma is also involved in the negative regulation of receptor tyrosine kinase-coupled signalling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD172 antigen-like family member B; CD172 gamma; CD172g; CD172g antigen; RP11-77C3.2; signal-regulatory protein beta 2; Signal-regulatory protein beta-2; Signal-regulatory protein gamma; SIRP beta 2; SIRP-b2; SIRP-beta-2; SIRP-gamma
Gene Aliases: bA77C3.1; CD172g; SIRP-B2; SIRPB2; SIRPG; SIRPgamma
UniProt ID: (Human) Q9P1W8
Entrez Gene ID: (Human) 55423
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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