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SIRT3 Antibody (PA5-95202) in WB
Western blot analysis of SIRT3 in Lane 1: rat spleen tissue lysate (50 µg), Lane 2: human placenta tissue lysate (50 µg), Lane 3: HeLa whole cell lysate (40 µg), Lane 4: COLO320 whole cell lysate (40 µg). Samples were incubated with SIRT3 polyclonal antibody (Product # PA5-95202).
Product Details
PA5-95202
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human SIRT3 recombinant protein (Position: P66-K399).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2807006
Product Specific Information
Human SIRT3 shares 86% amino acid (aa) sequence identity with mouse SIRT3.
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
SIRT3 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The SIRT3 is included in class I of the sirtuin family.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HGNC:14931; hSIRT3; mitochondrial nicotinamide adenine dinucleotide-dependent deacetylase; NAD-dependent deacetylase sirtuin-3, mitochondrial; NAD-dependent protein deacetylase sirtuin-3, mitochondrial; Regulatory protein SIR2 homolog 3; silent mating type information regulation 2, S.cerevisiae, homolog 3; sir2-like 3; SIR2-like protein 3; SIRT3L mitochondrial; sirtuin type 3
Gene Aliases: SIR2L3; SIRT3
UniProt ID: (Human) Q9NTG7
Entrez Gene ID: (Human) 23410, (Rat) 293615
protein ADP-ribosylation
protein deacetylation
mitochondrion organization
aerobic respiration
peptidyl-lysine deacetylation
histone H3 deacetylation
negative regulation of peptidyl-lysine acetylation
aging
positive regulation of insulin secretion
negative regulation of ERK1 and ERK2 cascade
negative regulation of reactive oxygen species metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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