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Invitrogen
SIRT6 Monoclonal Antibody (2G1H1)
Advanced Verification
This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 7
SIRT6 Antibody (MA1-214) in ICC/IF
Immunofluorescent analysis of SIRT6 (green) in MCF7 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes at -20c, permeabilized with 0.1% Triton X-100 for 15 minutes, and blocked with 3% BSA for 30 minutes at room temperature. Cells were stained with a SIRT6 mouse monoclonal antibody (Product # MA1-214) at a dilution of 1:100 in blocking buffer overnight at 4°C, and then incubated with a Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor Plus 488 conjugate (Product # A32731) at a dilution of 1:1000 for at least 30 min... View More
Product Details
MA1-214
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Mouse
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
2G1H1
Immunogen
Purified recombinant fragment of human SIRT6 (AA: 141-250) expressed in E. Coli.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2725358
Product Specific Information
MA1-214 SIRT6 antibody detects SIRT6 by western blot and immunofluroescence.
MA1-214 SIRT6 antibody detects SIRT6 by western blot at ~42kDa.
Target Information
Sirtuins regulate epigenetic gene silencing. SIRT6 (Mono-ADP-ribosyltransferase sirtuin-6), a mouse Sir2 homolog, is a chromatin-associated nuclear ADP-ribosyltransferase protein. It appears to be involved in DNA repair and may also play a role in human aging.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: hSIRT6; NAD-dependent protein deacetylase sirtuin-6; NAD-dependent protein deacylase sirtuin-6; Protein mono-ADP-ribosyltransferase sirtuin-6; Regulatory protein SIR2 homolog 6; SIR2; SIR2 like 6; SIR2-like protein 6; sir2-related protein type 6; sirtuin type 6
Gene Aliases: SIR2L6; SIRT6
UniProt ID: (Human) Q8N6T7
Entrez Gene ID: (Human) 51548
chromatin binding
transcription corepressor activity
NAD+ ADP-ribosyltransferase activity
NAD(P)+-protein-arginine ADP-ribosyltransferase activity
protein binding
zinc ion binding
NAD-dependent histone deacetylase activity
NAD-dependent protein deacetylase activity
NAD-dependent histone deacetylase activity (H3-K9 specific)
NAD+ binding
post-embryonic cardiac muscle cell growth involved in heart morphogenesis
base-excision repair
protein ADP-ribosylation
negative regulation of cell proliferation
regulation of double-strand break repair via homologous recombination
protein destabilization
response to nutrient levels
glucose homeostasis
negative regulation of glycolytic process
negative regulation of transcription, DNA-templated
negative regulation of glucose import
positive regulation of fibroblast proliferation
histone H3-K9 modification
histone H3-K9 deacetylation
positive regulation of stem cell proliferation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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