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SIT Antibody (MA1-19285) in WB
Western blotting analysis of human SIT using mouse monoclonal antibody SIT-01 on lysates of Molt-4 and HEK-293T cells under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2µg/mL of mouse anti-SIT Monoclonal antibody (Product # MA1-192850) followed by IRDye800-conjugated anti-mouse secondary antibody. SIT was detected around 36kDa.
Product Details
MA1-19285
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
SIT-01
Immunogen
Bacterially produced, recombinant, intracellular fragment of human SIT.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
15mM sodium azide
Storage conditions
4° C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2301877
Product Specific Information
This antibody reacts with murine SIT.
Western Blot: Reducing conditions.
Target Information
SIT (SHP2-interacting transmembrane adaptor protein) is expressed exclusively in lymphoid organs and acts either as a positive or as a negative regulatory element in T cell activation and in T cell development. Binding to Grb2 plays a pivotal role in signal transduction. Hubener et al. (2001) determined that the SIT gene contains 5 exons and spans 1.8 kb of genomic DNA. The SIT promoter demonstrated strong transcriptional activity and potential binding sites for both ubiquitous and lymphoid-specific transcription factors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: gp30/40; MGC125908; MGC125909; MGC125910; RP11-331F9.5; SHP-2 interacting transmembrane adaptor protein; SHP2 interacting transmembrane adaptor; SHP2-interacting transmembrane adapter protein; SHP2-interacting transmembrane adaptor protein; Signaling threshold-regulating transmembrane adapter 1; suppression inducing transmembrane adaptor 1; Suppression-inducing transmembrane adapter 1
Gene Aliases: SIT; SIT-R; SIT1
UniProt ID: (Human) Q9Y3P8
Entrez Gene ID: (Human) 27240
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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