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SIX3 Antibody (CF811410) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SIX3 (RC216286, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-SIX3. (1. Positive lysates LY401662 (100 µg) and LC401662 (20 µg) can be purchased separately from OriGene.
Product Details
CF811410
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI7G9
Immunogen
Human recombinant protein fragment corresponding to amino acids 142-332 of human SIX3 produced in E.coli.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 8% trehalose
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
This gene encodes a member of the sine oculis homeobox transcription factor family. The encoded protein plays a role in eye development. Mutations in this gene have been associated with holoprosencephaly type 2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Homeobox protein SIX3; Sine oculis homeobox homolog 3; sine oculis homeobox-like protein 3; sine oculis-related homeobox 3 homolog
Gene Aliases: E130112M24Rik; HPE2; SIX3; Six3a; Six3alpha; Six3b; Six3beta
UniProt ID: (Human) O95343, (Mouse) Q62233
Entrez Gene ID: (Human) 6496, (Mouse) 20473, (Rat) 78974
RNA polymerase II distal enhancer sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II distal enhancer sequence-specific binding
transcription corepressor binding
transcription factor activity, sequence-specific DNA binding
transcription coactivator activity
protein binding
histone deacetylase binding
DNA binding
transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
receptor binding
sequence-specific DNA binding
homeodomain transcription factor
protein import into nucleus, translocation
eye development
epithelial cell maturation
lens development in camera-type eye
optic vesicle morphogenesis
transcription from RNA polymerase II promoter
brain development
visual perception
proximal/distal axis specification
neuroblast differentiation
telencephalon development
forebrain anterior/posterior pattern specification
forebrain dorsal/ventral pattern formation
cell proliferation in forebrain
telencephalon regionalization
pituitary gland development
negative regulation of Wnt signaling pathway
regulation of cell proliferation
negative regulation of neuron differentiation
negative regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
circadian behavior
lens induction in camera-type eye
regulation of neural retina development
lens fiber cell differentiation
neuroblast migration
regulation of cell cycle phase transition
regulation of neuroblast proliferation
apoptotic process involved in development
lens fiber cell apoptotic process
regulation of neural precursor cell proliferation
transcription, DNA-templated
regulation of transcription, DNA-templated
protein import into nucleus
multicellular organism development
diencephalon development
camera-type eye development
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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