Proteintech

SKAP2 Polyclonal Antibody

SKAP2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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SKAP2 Antibody (12926-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 12926-1-AP ( SKAP2 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

SKAP2 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

12926-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 13 publications 13 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

View 5 publications 5 publications

Flow Cytometry (Flow)

Assay-dependent

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

SKAP2 Fusion Protein Ag3615 (1-359 aa encoded by BC036044)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.31 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MPNPSSTSS PYPLPEEIRN LLADVETFVA DILKGENLSK KAKEKRESLI KKIKDVKSIY LQEFQDKGDA EDGEEYDDPF AGPPDTISLA SERYDKDDEA PSDGAQFPPI AAQDLPFVLK AGYLEKRRKD HSFLGFEWQK RWCALSKTVF YYYGSDKDKQ QKGEFAIDGY SVRMNNTLRK DGKKDCCFEI SAPDKRIYQF TAASPKDAEE WVQQLKFVLQ DMESDIIPED YDERGELYDD VDHPLPISNP LTSSQPIDDE IYEELPEEEE DSAPVKVEEQ RKMSQDSVHH TSGDKSTDYA NFYQGLWDCT GAFSDELSFK RGDVIYILSK EYNRYGWWVG EMKGAIGLVP KAYIMEMYDI (1-359 aa encoded by BC036044)

Target Information

Fyb (Fyn binding protein) and the anchoring proteins SKAP55 and SKAP55-R (SKAP55-related protein) associate with the tyrosine kinase p59fyn. SKAP55 and SKAP55-R bind to Fyb through their SH3 domains and function as substrates for p59Fyn in resting T cells. SKAP55 contains an N-terminal pleckstrin homology domain and a C-terminal SH3 domain binding motif of adjacent arginine and lysine residues followed by tandem tyrosines (i.e. RKxxYxxY). SKAP55-R, similar in overall structure to SKAP55, contains a coiled-coil N-terminal domain. SKAP55 associates with SLAP-130, another component of the Fyn complex, which plays a role in the regulation of signaling events initiated by lymphocyte antigen receptors leading up to T cell activation. The human SKAP55 gene maps to chromosome 17q21.32 and encodes a 359 amino acid protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The mechanism of acentrosomal spindle assembly in human oocytes.

Science (New York, N.Y.)

Wu T,Dong J,Fu J,Kuang Y,Chen B,Gu H,Luo Y,Gu R,Zhang M,Li W,Dong X,Sun X,Sang Q,Wang L

Fri Nov 18 00:00:00 EST 2022

Hydrogen sulfide against preeclampsia exposure-induced oxidative mitochondrial damage in HTR-8/SVneo cells.

Frontiers in cardiovascular medicine

Wang X,Yu S,Jian Y,Pan H,Guo J,Wu J,Guo W

Fri Nov 18 00:00:00 EST 2022

Neutrophils require SKAP2 for reactive oxygen species production following C-type lectin and Candida stimulation.

iScience

Nguyen GT,Xu S,Adams W,Leong JM,Bunnell SC,Mansour MK,Sykes DB,Mecsas J

Fri Aug 20 00:00:00 EDT 2021

SKAP2 as a new regulator of oligodendroglial migration and myelin sheath formation.

Glia

Ghelman J,Grewing L,Windener F,Albrecht S,Zarbock A,Kuhlmann T

Mon Nov 01 00:00:00 EDT 2021

SKAP2, a Candidate Gene for Type 1 Diabetes, Regulates β-Cell Apoptosis and Glycemic Control in Newly Diagnosed Patients.

Diabetes

Fløyel T,Meyerovich K,Prause MC,Kaur S,Frørup C,Mortensen HB,Nielsen LB,Pociot F,Cardozo AK,Størling J

Mon Feb 01 00:00:00 EST 2021

SKAP2 is required for defense against K. pneumoniae infection and neutrophil respiratory burst.

eLife

Nguyen GT,Shaban L,Mack M,Swanson KD,Bunnell SC,Sykes DB,Mecsas J

Thu Apr 30 00:00:00 EDT 2020

Levobupivacaine inhibits proliferation and promotes apoptosis of breast cancer cells by suppressing the PI3K/Akt/mTOR signalling pathway.

BMC research notes

Kwakye AK,Kampo S,Lv J,Ramzan MN,Richard SA,Falagán AA,Agudogo J,Atito-Narh E,Yan Q,Wen QP

Mon Aug 17 00:00:00 EDT 2020

Scorpion Venom Analgesic Peptide, BmK AGAP Inhibits Stemness, and Epithelial-Mesenchymal Transition by Down-Regulating PTX3 in Breast Cancer.

Frontiers in oncology

Kampo S,Ahmmed B,Zhou T,Owusu L,Anabah TW,Doudou NR,Kuugbee ED,Cui Y,Lu Z,Yan Q,Wen QP

Tue Sep 19 00:00:00 EDT 2023

Skap2 is required for β₂ integrin-mediated neutrophil recruitment and functions.

The Journal of experimental medicine

Boras M,Volmering S,Bokemeyer A,Rossaint J,Block H,Bardel B,Van Marck V,Heitplatz B,Kliche S,Reinhold A,Lowell C,Zarbock A

Mon Mar 06 00:00:00 EST 2017

Skap2 is required for β₂ integrin-mediated neutrophil recruitment and functions.

The Journal of experimental medicine

Boras M,Volmering S,Bokemeyer A,Rossaint J,Block H,Bardel B,Van Marck V,Heitplatz B,Kliche S,Reinhold A,Lowell C,Zarbock A

Mon Mar 06 00:00:00 EST 2017

Skap2 is required for β₂ integrin-mediated neutrophil recruitment and functions.

The Journal of experimental medicine

Boras M,Volmering S,Bokemeyer A,Rossaint J,Block H,Bardel B,Van Marck V,Heitplatz B,Kliche S,Reinhold A,Lowell C,Zarbock A

Mon Mar 06 00:00:00 EST 2017

Skap2 is required for β₂ integrin-mediated neutrophil recruitment and functions.

The Journal of experimental medicine

Boras M,Volmering S,Bokemeyer A,Rossaint J,Block H,Bardel B,Van Marck V,Heitplatz B,Kliche S,Reinhold A,Lowell C,Zarbock A

Mon Mar 06 00:00:00 EST 2017

SKAP2 regulates Arp2/3 complex for actin-mediated asymmetric cytokinesis by interacting with WAVE2 in mouse oocytes.

Cell cycle (Georgetown, Tex.)

He SW,Xu BH,Liu Y,Wang YL,Chen MH,Xu L,Liao BQ,Lui R,Li FP,Lin YH,Fu XP,Fu BB,Hong ZW,Liu YX,Qi ZQ,Wang HL

Tue Jul 02 00:00:00 EDT 2019

SKAP2 regulates Arp2/3 complex for actin-mediated asymmetric cytokinesis by interacting with WAVE2 in mouse oocytes.

Cell cycle (Georgetown, Tex.)

He SW,Xu BH,Liu Y,Wang YL,Chen MH,Xu L,Liao BQ,Lui R,Li FP,Lin YH,Fu XP,Fu BB,Hong ZW,Liu YX,Qi ZQ,Wang HL

Tue Jul 02 00:00:00 EDT 2019

SKAP2 regulates Arp2/3 complex for actin-mediated asymmetric cytokinesis by interacting with WAVE2 in mouse oocytes.

Cell cycle (Georgetown, Tex.)

He SW,Xu BH,Liu Y,Wang YL,Chen MH,Xu L,Liao BQ,Lui R,Li FP,Lin YH,Fu XP,Fu BB,Hong ZW,Liu YX,Qi ZQ,Wang HL

Tue Jul 02 00:00:00 EDT 2019

SKAP2 regulates Arp2/3 complex for actin-mediated asymmetric cytokinesis by interacting with WAVE2 in mouse oocytes.

Cell cycle (Georgetown, Tex.)

He SW,Xu BH,Liu Y,Wang YL,Chen MH,Xu L,Liao BQ,Lui R,Li FP,Lin YH,Fu XP,Fu BB,Hong ZW,Liu YX,Qi ZQ,Wang HL

Tue Jul 02 00:00:00 EDT 2019

SKAP2 Promotes Podosome Formation to Facilitate Tumor-Associated Macrophage Infiltration and Metastatic Progression.

Cancer research

Tanaka M,Shimamura S,Kuriyama S,Maeda D,Goto A,Aiba N

Fri Jan 15 00:00:00 EST 2016

SKAP2 Promotes Podosome Formation to Facilitate Tumor-Associated Macrophage Infiltration and Metastatic Progression.

Cancer research

Tanaka M,Shimamura S,Kuriyama S,Maeda D,Goto A,Aiba N

Fri Jan 15 00:00:00 EST 2016

Ethylacetate extract from Tetrastigma hemsleyanum induces apoptosis via the mitochondrial caspase-dependent intrinsic pathway in HepG2 cells.

Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine

Peng X,Zhang YY,Wang J,Ji Q

Fri Jan 01 00:00:00 EST 2016

Ethylacetate extract from Tetrastigma hemsleyanum induces apoptosis via the mitochondrial caspase-dependent intrinsic pathway in HepG2 cells.

Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine

Peng X,Zhang YY,Wang J,Ji Q

Fri Jan 01 00:00:00 EST 2016

Src kinase-associated phosphoprotein2 expression is associated with poor prognosis in non-small cell lung cancer.

Anticancer research

Kuranami S,Yokobori T,Mogi A,Altan B,Yajima T,Onozato R,Azuma Y,Iijima M,Kosaka T,Kuwano H

Wed Apr 01 00:00:00 EDT 2015

Src kinase-associated phosphoprotein2 expression is associated with poor prognosis in non-small cell lung cancer.

Anticancer research

Kuranami S,Yokobori T,Mogi A,Altan B,Yajima T,Onozato R,Azuma Y,Iijima M,Kosaka T,Kuwano H

Wed Apr 01 00:00:00 EDT 2015

Identifying Yersinia YopH-targeted signal transduction pathways that impair neutrophil responses during in vivo murine infection.

Cell host & microbe

Rolán HG,Durand EA,Mecsas J

Wed Sep 11 00:00:00 EDT 2013

Identifying Yersinia YopH-targeted signal transduction pathways that impair neutrophil responses during in vivo murine infection.

Cell host & microbe

Rolán HG,Durand EA,Mecsas J

Wed Sep 11 00:00:00 EDT 2013

The Src substrate SKAP2 regulates actin assembly by interacting with WAVE2 and cortactin proteins.