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FIGURE: 1 / 9
SKAP2 Antibody (PA5-100004) in ICC/IF
Immunofluorescent analysis of SKAP2 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and SKAP2 polyclonal antibody (Product # PA5-100004) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Product Details
PA5-100004
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human SKAP2(Accession O75563), corresponding to amino acid residues I326-I359.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2815534
Product Specific Information
Antibody detects endogenous levels of total SKAP2.
Target Information
Fyb (Fyn binding protein) and the anchoring proteins SKAP55 and SKAP55-R (SKAP55-related protein) associate with the tyrosine kinase p59fyn. SKAP55 and SKAP55-R bind to Fyb through their SH3 domains and function as substrates for p59Fyn in resting T cells. SKAP55 contains an N-terminal pleckstrin homology domain and a C-terminal SH3 domain binding motif of adjacent arginine and lysine residues followed by tandem tyrosines (i.e. RKxxYxxY). SKAP55-R, similar in overall structure to SKAP55, contains a coiled-coil N-terminal domain. SKAP55 associates with SLAP-130, another component of the Fyn complex, which plays a role in the regulation of signaling events initiated by lymphocyte antigen receptors leading up to T cell activation. The human SKAP55 gene maps to chromosome 17q21.32 and encodes a 359 amino acid protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Fyn-associated phosphoprotein SKAP55 homologue; MGC10411; MGC33304; Pyk2/RAFTK-associated protein; Retinoic acid-induced protein 70; SKAP-55HOM; SKAP-HOM; SKAP55 homolog; SKAP55 homologue; SKAP55-HOM; src family associated phosphoprotein 2; Src family-associated phosphoprotein 2; Src kinase-associated phosphoprotein 2; Src kinase-associated phosphoprotein 55-related protein; src kinase-associated phosphoprotein of 55-related protein; Src-associated adapter protein with PH and SH3 domains; Src-associated adaptor protein
Gene Aliases: 2610021A10Rik; AA960083; BB137539; mSKAP55R; PRAP; RA70; SAPS; SCAP2; Scap55r; SKAP-HOM; SKAP2; SKAP55R
UniProt ID: (Human) O75563, (Rat) Q920G0, (Mouse) Q3UND0
Entrez Gene ID: (Human) 8935, (Rat) 155183, (Mouse) 54353
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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