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SKAP2 Antibody (PA5-21757) in ICC/IF
SKAP2 Polyclonal Antibody detects SKAP2 protein at cytoplasm by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SKAP2 protein stained by SKAP2 Polyclonal Antibody (Product # PA5-21757) diluted at 1:500. Blue: Hoechst 33342 staining.
Product Details
PA5-21757
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to a region within amino acids 296 and 359 of SCAP2 (Uniprot ID#O75563)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_11154020
Product Specific Information
Recommended positive controls: HepG2.
Predicted reactivity: Rhesus Monkey (100%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Fyb (Fyn binding protein) and the anchoring proteins SKAP55 and SKAP55-R (SKAP55-related protein) associate with the tyrosine kinase p59fyn. SKAP55 and SKAP55-R bind to Fyb through their SH3 domains and function as substrates for p59Fyn in resting T cells. SKAP55 contains an N-terminal pleckstrin homology domain and a C-terminal SH3 domain binding motif of adjacent arginine and lysine residues followed by tandem tyrosines (i.e. RKxxYxxY). SKAP55-R, similar in overall structure to SKAP55, contains a coiled-coil N-terminal domain. SKAP55 associates with SLAP-130, another component of the Fyn complex, which plays a role in the regulation of signaling events initiated by lymphocyte antigen receptors leading up to T cell activation. The human SKAP55 gene maps to chromosome 17q21.32 and encodes a 359 amino acid protein.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Fyn-associated phosphoprotein SKAP55 homologue; MGC10411; MGC33304; Pyk2/RAFTK-associated protein; Retinoic acid-induced protein 70; SKAP-55HOM; SKAP55 homolog; Src family-associated phosphoprotein 2; Src kinase-associated phosphoprotein 2; Src kinase-associated phosphoprotein 55-related protein; src kinase-associated phosphoprotein of 55-related protein; Src-associated adapter protein with PH and SH3 domains
Gene Aliases: PRAP; RA70; SAPS; SCAP2; SKAP-HOM; SKAP2; SKAP55R
UniProt ID: (Human) O75563
Entrez Gene ID: (Human) 8935
Performance Guarantee
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