Proteintech

SKP2 Polyclonal Antibody

SKP2 Antibody in Immunocytochemistry (ICC/IF)

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SKP2 Antibody (15010-1-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells using 15010-1-AP (SKP2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

SKP2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

SKP2 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

15010-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 34 publications 34 publications

Immunohistochemistry (IHC)

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:400

Immunocytochemistry (ICC/IF)

1:10-1:100

View 4 publications 4 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Not applicable (N/A)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

SKP2 Fusion Protein Ag6826 (1-344 aa encoded by BC001441)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.18 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MHRKHLQEI PDLSSNVATS FTWGWDSSKT SELLSGMGVS ALEKEEPDSE NIPQELLSNL GHPESPPRKR LKSKGSDKDF VIVRRPKLNR ENFPGVSWDS LPDELLLGIF SCLCLPELLK VSGVCKRWYR LASDESLWQT LDLTGKNLHP DVTGRLLSQG VIAFRCPRSF MDQPLAEHFS PFRVQHMDLS NSVIEVSTLH GILSQCSKLQ NLSLEGLRLS DPIVNTLAKN SNLVRLNLSG CSGFSEFALQ TLLSSCSRLD ELNLSWCFDF TEKHVQVAVA HVSETITQLN LSGYRKNLQK SDLSTLVRRC PNLVHLDLSD SVMLKNDCFQ EFFQLNYLQH LSLSR (1-344 aa encoded by BC001441)

Target Information

Skp2 (S-phase Kinase-associated Protein 2) belongs to the family of F-box proteins that interact with the Cyclin A-Cdk2 complex. Skp2 is essential for the G1-S transition in both transformed cells and diploid fibroblasts. Biochemical and genetic experiments have demonstrated that Skp2 is required for the ubiquitination and consequent degradation of p27 in cultured mammalian cells and in vitro reconstitution assays. In normal tissues, Skp2 is expressed in tonsil and placenta. In tumor tissues, Skp2 is expressed widely, in some colon, prostate, pancreas, and skin cancers, with high expression in lung, breast, ovarian, and endometrial cancers. Research suggests that Skp2 plays a role in oncogenesis, particularly in the pathogenesis of lymphomas and breast cancers.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Ethyl acetate extract of Antenoron Filiforme inhibits the proliferation of triple negative breast cancer cells via suppressing Skp2/p21 signaling axis.

Phytomedicine : international journal of phytotherapy and phytopharmacology

Liao Y,Li S,An J,Yu X,Tan X,Gui Y,Wang Y,Huang L,Zhou S,Wang D

Tue Jul 25 00:00:00 EDT 2023

ARIH2 regulates the proliferation, DNA damage and chemosensitivity of gastric cancer cells by reducing the stability of p21 via ubiquitination.

Cell death & disease

Geng S,Peng W,Wang X,Hu X,Liang H,Hou J,Wang F,Zhao G,Lü M,Cui H

Wed Jun 22 00:00:00 EDT 2022

The SKP2-p27 axis defines susceptibility to cell death upon CHK1 inhibition.

Molecular oncology

Lohmüller M,Roeck BF,Szabo TG,Schapfl MA,Pegka F,Herzog S,Villunger A,Schuler F

Mon Aug 01 00:00:00 EDT 2022

PI3K inhibitor 3-MA promotes the antiproliferative activity of esomeprazole in gastric cancer cells by downregulating EGFR via the PI3K/FOXO3a pathway.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Du J,Xu Q,Zhao H,Jia X,Ba N,Peng F,Zhang Z

Fri Apr 01 00:00:00 EDT 2022

Aurora-A/FOXO3A/SKP2 axis promotes tumor progression in clear cell renal cell carcinoma and dual-targeting Aurora-A/SKP2 shows synthetic lethality.

Cell death & disease

Li P,Chen T,Kuang P,Liu F,Li Z,Liu F,Wang Y,Zhang W,Cai X

Wed Jul 13 00:00:00 EDT 2022

SCF-SKP2 E3 ubiquitin ligase links mTORC1/ER stress/ISR with YAP activation in murine renal cystogenesis.

The Journal of clinical investigation

Panda DK,Bai X,Zhang Y,Stylianesis NA,Koromilas AE,Lipman ML,Karaplis AC

Thu Dec 15 00:00:00 EST 2022

SKP2 drives the sensitivity to neddylation inhibitors and cisplatin in malignant pleural mesothelioma.

Journal of experimental & clinical cancer research : CR

Salaroglio IC,Belisario DC,Bironzo P,Ananthanarayanan P,Ricci L,Digiovanni S,Fontana S,Napoli F,Sandri A,Facolmatà C,Libener R,Comunanza V,Grosso F,Gazzano E,Leo F,Taulli R,Bussolino F,Righi L,Papotti MG,Novello S,Scagliotti GV,Riganti C,Kopecka J

Wed Feb 23 00:00:00 EST 2022

p97/VCP is highly expressed in the stem-like cells of breast cancer and controls cancer stemness partly through the unfolded protein response.

Cell death & disease

Li C,Huang Y,Fan Q,Quan H,Dong Y,Nie M,Wang J,Xie F,Ji J,Zhou L,Zheng Z,Wang L

Wed Mar 17 00:00:00 EDT 2021

p97/VCP is highly expressed in the stem-like cells of breast cancer and controls cancer stemness partly through the unfolded protein response.

Cell death & disease

Li C,Huang Y,Fan Q,Quan H,Dong Y,Nie M,Wang J,Xie F,Ji J,Zhou L,Zheng Z,Wang L

Wed Mar 17 00:00:00 EDT 2021

Stimulation of ROS Generation by Extract of Warburgia ugandensis Leading to G₀/G₁ Cell Cycle Arrest and Antiproliferation in A549 Cells.

Antioxidants (Basel, Switzerland)

Zhang YL,Chen GL,Liu Y,Zhuang XC,Guo MQ

Thu Sep 30 00:00:00 EDT 2021

Potential Role of SWI/SNF Complex Subunit Actin-Like Protein 6A in Cervical Cancer.

Frontiers in oncology

Wang Q,Cao Z,Wei Y,Zhang J,Cheng Z

Tue Aug 17 00:00:00 EDT 2021

Exenatide improves random-pattern skin flap survival via TFE3 mediated autophagy augment.

Journal of cellular physiology

Li J,Chen H,Lou J,Bao G,Wu C,Lou Z,Wang X,Ding J,Li Z,Xiao J,Xu H,Gao W,Zhou K

Sat May 01 00:00:00 EDT 2021

MAD2B-mediated cell cycle reentry of podocytes is involved in the pathogenesis of FSGS.

International journal of biological sciences

Bao D,Su H,Lei CT,Tang H,Ye C,Xiong W,He FF,Lin JH,Hammes HP,Zhang C

Mon Dec 20 00:00:00 EST 2021

MAD2B-mediated cell cycle reentry of podocytes is involved in the pathogenesis of FSGS.

International journal of biological sciences

Bao D,Su H,Lei CT,Tang H,Ye C,Xiong W,He FF,Lin JH,Hammes HP,Zhang C

Mon Dec 20 00:00:00 EST 2021

The interaction of SKP2 with p27 enhances the progression and stemness of osteosarcoma.

Annals of the New York Academy of Sciences

Wang J,Aldahamsheh O,Ferrena A,Borjihan H,Singla A,Yaguare S,Singh S,Viscarret V,Tingling J,Zi X,Lo Y,Gorlick R,Zheng D,Schwartz EL,Zhao H,Yang R,Geller DS,Hoang BH

Thu Apr 01 00:00:00 EDT 2021

Structure-Guided Design of a Small-Molecule Activator of Sirtuin-3 that Modulates Autophagy in Triple Negative Breast Cancer.

Journal of medicinal chemistry

Zhang J,Zou L,Shi D,Liu J,Zhang J,Zhao R,Wang G,Zhang L,Ouyang L,Liu B

Thu Oct 14 00:00:00 EDT 2021

β-RA Targets Mitochondrial Metabolism and Adipogenesis, Leading to Therapeutic Benefits against CoQ Deficiency and Age-Related Overweight.

Biomedicines

Hidalgo-Gutiérrez A,Barriocanal-Casado E,Díaz-Casado ME,González-García P,Zenezini Chiozzi R,Acuña-Castroviejo D,López LC

Wed Oct 13 00:00:00 EDT 2021

Nobiletin downregulates the SKP2-p21/p27-CDK2 axis to inhibit tumor progression and shows synergistic effects with palbociclib on renal cell carcinoma.

Cancer biology & medicine

Chen T,Liu L,Zou Y,Hu X,Zhang W,Zhou T,Luo X,Fu W,Xu J

Mon Feb 15 00:00:00 EST 2021

ZNF292 suppresses proliferation of ESCC cells through ZNF292/SKP2/P27 signaling axis.

Chinese journal of cancer research = Chung-kuo yen cheng yen chiu

Gong W,Xu J,Wang G,Li D,Zhan Q

Fri Dec 31 00:00:00 EST 2021

A nonautophagic role of ATG5 in regulating cell growth by targeting c-Myc for proteasome-mediated degradation.

iScience

Li S,Zhang L,Zhang G,Shangguan G,Hou X,Duan W,Xi Y,Xu N,Zhang B,Dong J,Wang Y,Cui W,Chen S

Fri Nov 19 00:00:00 EST 2021

DNAJC5 promotes hepatocellular carcinoma cells proliferation though regulating SKP2 mediated p27 degradation.

Biochimica et biophysica acta. Molecular cell research

Wang H,Luo J,Tian X,Xu L,Zhai Z,Cheng M,Chen L,Luo S

Sat May 01 00:00:00 EDT 2021

CircCRIM1 Promotes Hepatocellular Carcinoma Proliferation and Angiogenesis by Sponging miR-378a-3p and Regulating SKP2 Expression.

Frontiers in cell and developmental biology

Ji Y,Yang S,Yan X,Zhu L,Yang W,Yang X,Yu F,Shi L,Zhu X,Lu Y,Zhang C,Lu H,Zhang F

Tue Dec 07 00:00:00 EST 2021

USP10 exacerbates neointima formation by stabilizing Skp2 protein in vascular smooth muscle cells.

The Journal of biological chemistry

Xia X,Liu X,Chai R,Xu Q,Luo Z,Gu J,Jin Y,Hu T,Yu C,Du B,Huang H,Ou W,Liu S,Liu N

Mon Nov 01 00:00:00 EDT 2021

Inhibition of MAD2B alleviates venous neointimal formation by suppressing VSMCs proliferation and migration.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Song AN,Yin XJ,Gao P,Tang H,Meng XF,Zhang C

Mon Nov 01 00:00:00 EDT 2021

TFE3, a potential therapeutic target for Spinal Cord Injury via augmenting autophagy flux and alleviating ER stress.

Theranostics

Zhou K,Zheng Z,Li Y,Han W,Zhang J,Mao Y,Chen H,Zhang W,Liu M,Xie L,Zhang H,Xu H,Xiao J

Fri May 28 00:00:00 EDT 2021

Deferasirox protects against hydrogen peroxide-induced cell apoptosis by inhibiting ubiquitination and degradation of p21^WAF1/CIP1.

Biochemical and biophysical research communications

Miao J,Xu M,Kuang Y,Pan S,Hou J,Cao P,Duan X,Chang Y,Hasem H,Zhou N,Tan K,Fan Y

Thu Apr 09 00:00:00 EDT 2020

MAD2B contributes to parietal epithelial cell activation and crescentic glomerulonephritis via Skp2.

American journal of physiology. Renal physiology

Ye C,Xiong W,Lei CT,Tang H,Su H,Yi F,Zhang C

Thu Oct 01 00:00:00 EDT 2020

Inhibition of Skp2 Sensitizes Chronic Myeloid Leukemia Cells to Imatinib.

Cancer management and research

Chen X,Huang Z,Wu W,Xia R

Fri Apr 15 00:00:00 EDT 2022

Myc-mediated SDHA acetylation triggers epigenetic regulation of gene expression and tumorigenesis.

Nature metabolism

Li ST,Huang D,Shen S,Cai Y,Xing S,Wu G,Jiang Z,Hao Y,Yuan M,Wang N,Zhu L,Yan R,Yang D,Wang L,Liu Z,Hu X,Zhou R,Qu K,Li A,Duan X,Zhang H,Gao P

Sun Mar 01 00:00:00 EST 2020

Skp2 contributes to cell cycle progression in trophoblast stem cells and to placental development.

Genes to cells : devoted to molecular & cellular mechanisms

Yamauchi Y,Nita A,Nishiyama M,Muto Y,Shimizu H,Nakatsumi H,Nakayama KI

Mon Jun 01 00:00:00 EDT 2020

FGF21 augments autophagy in random-pattern skin flaps via AMPK signaling pathways and improves tissue survival.

Cell death & disease

Zhou K,Chen H,Lin J,Xu H,Wu H,Bao G,Li J,Deng X,Shui X,Gao W,Ding J,Xiao J,Xu H

Mon Nov 18 00:00:00 EST 2019

Regulation of circadian rhythms by NEAT1 mediated TMAO-induced endothelial proliferation: A protective role of asparagus extract.

Experimental cell research

Wu X,Chen L,Zeb F,Huang Y,An J,Ren J,Yang F,Feng Q

Sun Sep 01 00:00:00 EDT 2019

The ubiquitin ligase SCF^FBXW7α promotes GATA3 degradation.

Journal of cellular physiology

Song N,Cao C,Tang Y,Bi L,Jiang Y,Zhou Y,Song X,Liu L,Ge W

Thu Mar 01 00:00:00 EST 2018

The ubiquitin ligase SCF^FBXW7α promotes GATA3 degradation.

Journal of cellular physiology

Song N,Cao C,Tang Y,Bi L,Jiang Y,Zhou Y,Song X,Liu L,Ge W

Thu Mar 01 00:00:00 EST 2018

Long non-coding RNA LINC-01572:28 inhibits granulosa cell growth via a decrease in p27 (Kip1) degradation in patients with polycystic ovary syndrome.

EBioMedicine

Zhao J,Xu J,Wang W,Zhao H,Liu H,Liu X,Liu J,Sun Y,Dunaif A,Du Y,Chen ZJ

Mon Oct 01 00:00:00 EDT 2018

The Interaction of miR-378i-Skp2 Regulates Cell Senescence in Diabetic Nephropathy.

Journal of clinical medicine

Tsai YC,Kuo PL,Kuo MC,Hung WW,Wu LY,Chang WA,Wu PH,Lee SC,Chen HC,Hsu YL

Thu Nov 22 00:00:00 EST 2018

Hippo Signaling Suppresses Cell Ploidy and Tumorigenesis through Skp2.

Cancer cell