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SKP2 Antibody (606-250) in IHC (P)
Immunohistochemistry: Human kidney tissue (FFPE) stained with Mouse anti-SKP2 (Cat#606-250) at 1:200 for 10 min @ RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Product Details
606-250
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
1G12E9
Immunogen
A recombinant protein human SKP2
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS with proprietary stabilizer
Contains
0.01% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
Product Specific Information
Positive control: Lymphoma
Cellular location: Nucleus.
Target Information
Skp2 (S-phase Kinase-associated Protein 2) belongs to the family of F-box proteins that interact with the Cyclin A-Cdk2 complex. Skp2 is essential for the G1-S transition in both transformed cells and diploid fibroblasts. Biochemical and genetic experiments have demonstrated that Skp2 is required for the ubiquitination and consequent degradation of p27 in cultured mammalian cells and in vitro reconstitution assays. In normal tissues, Skp2 is expressed in tonsil and placenta. In tumor tissues, Skp2 is expressed widely, in some colon, prostate, pancreas, and skin cancers, with high expression in lung, breast, ovarian, and endometrial cancers. Research suggests that Skp2 plays a role in oncogenesis, particularly in the pathogenesis of lymphomas and breast cancers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CDK2/cyclin A-associated protein p45; Cyclin-A/CDK2-associated protein p45; F-box protein Skp2; F-box/LRR-repeat protein 1; MGC1366; OTTHUMP00000221389; p45skp2; S-phase kinase-associated protein 2; S-phase kinase-associated protein 2 (p45)
Gene Aliases: FBL1; FBXL1; FLB1; p45; SKP2
UniProt ID: (Human) Q13309
Entrez Gene ID: (Human) 6502
G1/S transition of mitotic cell cycle
G2/M transition of mitotic cell cycle
protein polyubiquitination
cell proliferation
anaphase-promoting complex-dependent catabolic process
positive regulation of intracellular estrogen receptor signaling pathway
regulation of apoptotic process
positive regulation of smooth muscle cell proliferation
regulation of cell cycle
cellular response to cell-matrix adhesion
positive regulation of protein polyubiquitination
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