Product References
CRISPR-mediated knockout of VEGFR2/KDR inhibits cell growth in a squamous thyroid cancer cell line.
FEBS open bio
Tsai ML,Lee CH,Huang LC,Chen YH,Liu WN,Lin CY,Hsu KW,Lee AW,Lin CL
32-3300 was used in Western Blotting to suggest that selective targeting of KDR may have potential for development into novel anti-cancer therapies to suppress VEGF/VEGFR-mediated cancer development in patients with clinical advanced thyroid cancer.
Sun May 01 00:00:00 EDT 2022
Discriminative SKP2 Interactions with CDK-Cyclin Complexes Support a Cyclin A-Specific Role in p27KIP1 Degradation.
Journal of molecular biology
Salamina M,Montefiore BC,Liu M,Wood DJ,Heath R,Ault JR,Wang LZ,Korolchuk S,Baslé A,Pastok MW,Reeks J,Tatum NJ,Sobott F,Arold ST,Pagano M,Noble MEM,Endicott JA
32-3300 was used in Western Blot to identify the SKP2 binding site on cyclin A and demonstrate the site is not present in cyclin B or cyclin E.
Fri Mar 05 00:00:00 EST 2021
Artemisinin Mediates Its Tumor-Suppressive Activity in Hepatocellular Carcinoma Through Targeted Inhibition of FoxM1.
Frontiers in oncology
Nandi D,Cheema PS,Singal A,Bharti H,Nag A
32-3300 was used in Western Blot to study tumour suppression of hepatocellular carcinoma by antimalarial natural product Artemisinin.
Wed Dec 15 00:00:00 EST 2021
The Hippo Pathway Effector YAP Promotes Ferroptosis via the E3 Ligase SKP2.
Molecular cancer research : MCR
Yang WH,Lin CC,Wu J,Chao PY,Chen K,Chen PH,Chi JT
32-3300 was used in Western Blotting to reveal that YAP promotes ferroptosis by regulating SKP2, suggesting novel therapeutic options for YAP-driven tumors.
Tue Jun 01 00:00:00 EDT 2021
Downregulation of SRPK2 promotes cell cycle arrest though E2F1 in non-small cell lung cancer.
European journal of histochemistry : EJH
Li X,Yang S,Zhang M,Xie S,Xie Z
32-3300 was used in Western Blotting to show cell cycle arrest can be promoted by serine-arginine protein kinase downregulation in non-small cell lung cancer.
Wed Dec 11 00:00:00 EST 2019
ERAP1 promotes Hedgehog-dependent tumorigenesis by controlling USP47-mediated degradation of βTrCP.
Nature communications
Bufalieri F,Infante P,Bernardi F,Caimano M,Romania P,Moretti M,Lospinoso Severini L,Talbot J,Melaiu O,Tanori M,Di Magno L,Bellavia D,Capalbo C,Puget S,De Smaele E,Canettieri G,Guardavaccaro D,Busino L,Peschiaroli A,Pazzaglia S,Giannini G,Melino G,Locatelli F,Gulino A,Ayrault O,Fruci D,Di Marcotullio L
32-3300 was used in Immunohistochemistry to demonstrate the role of ERAP1 in the Hh signalling pathway and to show its effect in cancer and possible uses for therapeutic treatment.
Wed Jul 24 00:00:00 EDT 2019
Skp2-dependent reactivation of AKT drives resistance to PI3K inhibitors.
Science signaling
Clement E,Inuzuka H,Nihira NT,Wei W,Toker A
32-3300 was used in Western Blotting to reveal a ubiquitin signalling feedback mechanism by which PI3K inhibitor resistance may emerge in aggressive breast cancer cells.
Tue Mar 13 00:00:00 EDT 2018
TMPRSS2-ERG Controls Luminal Epithelial Lineage and Antiandrogen Sensitivity in PTEN and TP53-Mutated Prostate Cancer.
Clinical cancer research : an official journal of the American Association for Cancer Research
Blee AM,He Y,Yang Y,Ye Z,Yan Y,Pan Y,Ma T,Dugdale J,Kuehn E,Kohli M,Jimenez R,Chen Y,Xu W,Wang L,Huang H
32-3300 was used in Western Blotting to identify a previously undefined function of ERG to restrict lineage plasticity and maintain antiandrogen sensitivity in PTEN/TP53-altered prostate cancer.
Sat Sep 15 00:00:00 EDT 2018
Down-regulation of Skp2 expression inhibits invasion and lung metastasis in osteosarcoma.
Scientific reports
Zhang Y,Zvi YS,Batko B,Zaphiros N,O'Donnell EF,Wang J,Sato K,Yang R,Geller DS,Koirala P,Zhang W,Du X,Piperdi S,Liu Y,Zheng D,Roth M,Gill J,Zhang J,Ren T,Gorlick R,Zi X,Hoang BH
32-3300 was used in Western Blotting to elucidate the role of S phase kinase-associated protein in osteosarcoma invasion and metastasis, and to explore flavokawain A as a potential Skp2 targeting agent for preventing osteosarcoma progression.
Mon Sep 24 00:00:00 EDT 2018
SKP2- and OTUD1-regulated non-proteolytic ubiquitination of YAP promotes YAP nuclear localization and activity.
Nature communications
Yao F,Zhou Z,Kim J,Hang Q,Xiao Z,Ton BN,Chang L,Liu N,Zeng L,Wang W,Wang Y,Zhang P,Hu X,Su X,Liang H,Sun Y,Ma L
Mon Jun 11 00:00:00 EDT 2018
SIRT6-dependent cysteine monoubiquitination in the PRE-SET domain of Suv39h1 regulates the NF-κB pathway.
Nature communications
Santos-Barriopedro I,Bosch-Presegué L,Marazuela-Duque A,de la Torre C,Colomer C,Vazquez BN,Fuhrmann T,Martínez-Pastor B,Lu W,Braun T,Bober E,Jenuwein T,Serrano L,Esteller M,Chen Z,Barceló-Batllori S,Mostoslavsky R,Espinosa L,Vaquero A
32-3300 was used in Western Blotting to suggest a mechanism based on SirT6-mediated enhancement of a negative feedback loop that restricts the NF-κB pathway.
Tue Jan 09 00:00:00 EST 2018
The co-existence of transcriptional activator and transcriptional repressor MEF2 complexes influences tumor aggressiveness.
PLoS genetics
Di Giorgio E,Franforte E,Cefalù S,Rossi S,Dei Tos AP,Brenca M,Polano M,Maestro R,Paluvai H,Picco R,Brancolini C
32-3300 was used in Immunoprecipitation to clarify that MEF2 can support both pro-oncogenic or tumour suppressive activities depending on the interaction with co-activators or co-repressors partners.
Sat Apr 01 00:00:00 EDT 2017
The co-existence of transcriptional activator and transcriptional repressor MEF2 complexes influences tumor aggressiveness.
PLoS genetics
Di Giorgio E,Franforte E,Cefalù S,Rossi S,Dei Tos AP,Brenca M,Polano M,Maestro R,Paluvai H,Picco R,Brancolini C
32-3300 was used in Immunoprecipitation to clarify that MEF2 can support both pro-oncogenic or tumour suppressive activities depending on the interaction with co-activators or co-repressors partners.
Sat Apr 01 00:00:00 EDT 2017
TGF-β activates APC through Cdh1 binding for Cks1 and Skp2 proteasomal destruction stabilizing p27kip1 for normal endometrial growth.
Cell cycle (Georgetown, Tex.)
Pavlides SC,Lecanda J,Daubriac J,Pandya UM,Gama P,Blank S,Mittal K,Shukla P,Gold LI
32-3300 was used in western blot to investigate activation of APC through Cdh1 binding for Cks1 and Skp2 proteasomal destruction stabilizing p27kip1 for normal endometrial growth by TGF-beta
Mon Mar 06 00:00:00 EST 2017
SKP2 is a direct transcriptional target of MYCN and a potential therapeutic target in neuroblastoma.
Cancer letters
Evans L,Chen L,Milazzo G,Gherardi S,Perini G,Willmore E,Newell DR,Tweddle DA
32-3300 was used in western blot to study the relationship between SKP2 and MYCN
Fri Jul 10 00:00:00 EDT 2015
Exposure of neuroblastoma cell lines to imatinib results in the upregulation of the CDK inhibitor p27(KIP1) as a consequence of c-Abl inhibition.
Biochemical pharmacology
Lupino E,Ramondetti C,Buccinnà B,Piccinini M
32-3300 was used in western blot to study the effect of imatinib on neuroblastoma cell lines
Sat Nov 15 00:00:00 EST 2014
Epidermal growth factor upregulates Skp2/Cks1 and p27(kip1) in human extrahepatic cholangiocarcinoma cells.
World journal of gastroenterology
Kim JY,Kim HJ,Park JH,Park DI,Cho YK,Sohn CI,Jeon WK,Kim BI,Kim DH,Chae SW,Sohn JH
Tue Jan 21 00:00:00 EST 2014
BRD4 sustains melanoma proliferation and represents a new target for epigenetic therapy.
Cancer research
Segura MF,Fontanals-Cirera B,Gaziel-Sovran A,Guijarro MV,Hanniford D,Zhang G,González-Gomez P,Morante M,Jubierre L,Zhang W,Darvishian F,Ohlmeyer M,Osman I,Zhou MM,Hernando E
32-3300 was used in western blot to discover a critical role for BRD4 in melanoma tumor maintenance.
Tue Oct 15 00:00:00 EDT 2013
Modulation of AKT activity is associated with reversible dormancy in ascites-derived epithelial ovarian cancer spheroids.
Carcinogenesis
Correa RJ,Peart T,Valdes YR,DiMattia GE,Shepherd TG
32-3300 was used in western blot to investigate epithelial ovarian cancer metastasis.
Sun Jan 01 00:00:00 EST 2012
von Hippel-Lindau protein promotes Skp2 destabilization on DNA damage.
Oncogene
Roe JS,Kim HR,Hwang IY,Cho EJ,Youn HD
32-3300 was used in immunoprecipitation and western blot to elucidate the molecular mechanisms by which pVHL contributes to tumorigenesis.
Thu Jul 14 00:00:00 EDT 2011
von Hippel-Lindau protein promotes Skp2 destabilization on DNA damage.
Oncogene
Roe JS,Kim HR,Hwang IY,Cho EJ,Youn HD
32-3300 was used in immunoprecipitation and western blot to elucidate the molecular mechanisms by which pVHL contributes to tumorigenesis.
Thu Jul 14 00:00:00 EDT 2011
The human COP9 signalosome protects ubiquitin-conjugating enzyme 3 (UBC3/Cdc34) from beta-transducin repeat-containing protein (betaTrCP)-mediated degradation.
The Journal of biological chemistry
Fernandez-Sanchez ME,Sechet E,Margottin-Goguet F,Rogge L,Bianchi E
32-3300 was used in western blot to explore how the COP9 signalosome affects ubiquitination processes
Fri Jun 04 00:00:00 EDT 2010
Targeting the p27 E3 ligase SCF(Skp2) results in p27- and Skp2-mediated cell-cycle arrest and activation of autophagy.
Blood
Chen Q,Xie W,Kuhn DJ,Voorhees PM,Lopez-Girona A,Mendy D,Corral LG,Krenitsky VP,Xu W,Moutouh-de Parseval L,Webb DR,Mercurio F,Nakayama KI,Nakayama K,Orlowski RZ
Thu May 01 00:00:00 EDT 2008
Targeting the p27 E3 ligase SCF(Skp2) results in p27- and Skp2-mediated cell-cycle arrest and activation of autophagy.
Blood
Chen Q,Xie W,Kuhn DJ,Voorhees PM,Lopez-Girona A,Mendy D,Corral LG,Krenitsky VP,Xu W,Moutouh-de Parseval L,Webb DR,Mercurio F,Nakayama KI,Nakayama K,Orlowski RZ
Thu May 01 00:00:00 EDT 2008
Mutant B-RAF signaling and cyclin D1 regulate Cks1/S-phase kinase-associated protein 2-mediated degradation of p27Kip1 in human melanoma cells.
Oncogene
Bhatt KV,Hu R,Spofford LS,Aplin AE
32-3300 was used in western blot to study the role of B-RAF and cyclin D1 on p27Kip1 in human melanoma cells
Thu Feb 15 00:00:00 EST 2007
Disrupting Skp2-cyclin A interaction with a blocking peptide induces selective cancer cell killing.
Molecular cancer therapeutics
Ji P,Sun D,Wang H,Bauzon F,Zhu L
32-3300 was used in flow cytometry, immunocytochemistry, and western blot to report that the Skp2-cyclin A interaction has a major role in cancer cell survival
Thu Feb 01 00:00:00 EST 2007
The COP9 signalosome regulates Skp2 levels and proliferation of human cells.
The Journal of biological chemistry
Denti S,Fernandez-Sanchez ME,Rogge L,Bianchi E
32-3300 was used in western blot to examine how the COP9 signalosome regulates the cell cycle in human cells
Fri Oct 27 00:00:00 EDT 2006
Skp2 contains a novel cyclin A binding domain that directly protects cyclin A from inhibition by p27Kip1.
The Journal of biological chemistry
Ji P,Goldin L,Ren H,Sun D,Guardavaccaro D,Pagano M,Zhu L
32-3300 was used in western blot to determine the consequences of the Skp2-cyclin A interaction
Fri Aug 18 00:00:00 EDT 2006
The pT1a and pT1b category subdivision in renal cell carcinoma: is it reflected by differences in tumour biology?
BJU international
Langner C,Ratschek M,Rehak P,Tsybrovskyy O,Zigeuner R
32-3300 was used in immunohistochemistry - paraffin section to study the category subdivision of pT1a and pT1b in renal cell carcinoma
Tue Feb 01 00:00:00 EST 2005
Downregulation of Skp2 and p27/Kip1 synergistically induces apoptosis in T98G glioblastoma cells.
Journal of molecular medicine (Berlin, Germany)
Lee SH,McCormick F
32-3300 was used in western blot to analyze the synergistic induction of apoptosis in T98G glioblastoma cells due to downregulation of Skp2 and p27/Kip1
Fri Apr 01 00:00:00 EST 2005
Ubiquitination and proteolysis of cancer-derived Smad4 mutants by SCFSkp2.
Molecular and cellular biology
Liang M,Liang YY,Wrighton K,Ungermannova D,Wang XP,Brunicardi FC,Liu X,Feng XH,Lin X
Wed Sep 01 00:00:00 EDT 2004
Role of RhoA, mDia, and ROCK in cell shape-dependent control of the Skp2-p27kip1 pathway and the G1/S transition.
The Journal of biological chemistry
Mammoto A,Huang S,Moore K,Oh P,Ingber DE
32-3300 was used in western blot to report that RhoA conveys the "cell shape signal" to the cell-cycle machinery in human capillary endothelial cells
Fri Jun 18 00:00:00 EDT 2004
Tuberin binds p27 and negatively regulates its interaction with the SCF component Skp2.
The Journal of biological chemistry
Rosner M,Hengstschläger M
Fri Nov 19 00:00:00 EST 2004
Tuberin binds p27 and negatively regulates its interaction with the SCF component Skp2.
The Journal of biological chemistry
Rosner M,Hengstschläger M
Fri Nov 19 00:00:00 EST 2004
Cyclin-dependent kinases phosphorylate human Cdt1 and induce its degradation.
The Journal of biological chemistry
Liu E,Li X,Yan F,Zhao Q,Wu X
32-3300 was used in western blot to study phosphorylation of human Cdt1 and induction of its degradation due to cyclin-dependent kinases
Fri Apr 23 00:00:00 EDT 2004
The SCF(Skp2) ubiquitin ligase complex interacts with the human replication licensing factor Cdt1 and regulates Cdt1 degradation.
The Journal of biological chemistry
Li X,Zhao Q,Liao R,Sun P,Wu X
32-3300 was used in western blot to study the mechanisms of Cdt1 degradation in mammalian cells.
Fri Aug 15 00:00:00 EDT 2003
