Alomone Labs, LTD

SLC11A1/NRAMP1 (extracellular) Polyclonal Antibody

View all (10) SLC11A1 antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

ANT-201-200UL

Applications
Tested Dilution
Publications

Western Blot (WB)

1:200
-

Immunohistochemistry (Frozen) (IHC (F))

1:600
-

Flow Cytometry (Flow)

5 µg
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

CANSSLHDYAKIFP(R), corresponding to amino acid residues 322-335 of human SLC11A1, 4th extracellular loop
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.8 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 1% BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Information

NRAMP1, also known as SLC11A1, is a member of the solute carrier family 11 (proton-coupled divalent metal ion transporters) family and encodes a multi-pass membrane protein. The protein functions as a divalent transition metal (iron and manganese) transporter involved in iron metabolism and host resistance to certain pathogens. Mutations in this gene have been associated with susceptibility to infectious diseases such as leprosy and tuberculosis, and inflammatory diseases such as Crohn disease and rheumatoid arthritis. Alternatively spliced variants that encode different protein isoforms have been described but the full-length nature of only one has been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: host resistance locus Bcg/Ity/Lsh; natural resistance associated macrophage protein 1; Natural resistance-associated macrophage protein 1; NRAMP 1; solute carrier family 11 (proton-coupled divalent metal ion transporter), member 1; solute carrier family 11 (proton-coupled divalent metal ion transporters), member 1; solute carrier family 11 (sodium/phosphate symporters), member 1; Solute carrier family 11 member 1

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Gene Aliases: Bcg; Itg; Ity; LSH; NRAMP; NRAMP1; SLC11A1

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UniProt ID: (Human) P49279, (Mouse) P41251, (Rat) P70553

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Entrez Gene ID: (Human) 6556, (Mouse) 18173, (Rat) 316519

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Function(s)
manganese ion transmembrane transporter activity protein homodimerization activity transition metal ion transmembrane transporter activity metal ion:proton antiporter activity transporter activity molecular_function secondary carrier transporter
Process(es)
protein import into nucleus, translocation negative regulation of cytokine production positive regulation of cytokine production T cell proliferation involved in immune response T cell cytokine production positive regulation of dendritic cell antigen processing and presentation positive regulation of T-helper 1 type immune response iron ion transport manganese ion transport cellular cadmium ion homeostasis cellular iron ion homeostasis phagocytosis inflammatory response vacuolar acidification response to bacterium positive regulation of gene expression nitrite transport activation of protein kinase activity response to lipopolysaccharide interleukin-2 production interleukin-3 production positive regulation of interferon-gamma production response to interferon-gamma wound healing macrophage activation defense response to bacterium defense response to protozoan L-arginine import MHC class II biosynthetic process cell redox homeostasis respiratory burst positive regulation of transcription from RNA polymerase II promoter antigen processing and presentation of peptide antigen mRNA stabilization positive regulation of phagocytosis defense response to Gram-negative bacterium iron ion homeostasis multicellular organismal iron ion homeostasis cadmium ion transmembrane transport divalent metal ion export manganese ion transmembrane transport MAPK cascade transport ion transport hydrogen ion transmembrane transport biological_process
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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